Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN3VN_RS04955 Genome accession   NZ_CP183396
Coordinates   1072340..1073374 (-) Length   344 a.a.
NCBI ID   WP_046419718.1    Uniprot ID   -
Organism   Xylella fastidiosa strain CFBP8074     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1067340..1078374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN3VN_RS04940 (ACN3VN_04940) - 1067861..1068670 (-) 810 WP_021358437.1 DUF481 domain-containing protein -
  ACN3VN_RS04945 (ACN3VN_04945) - 1069514..1070527 (+) 1014 Protein_959 helix-turn-helix transcriptional regulator -
  ACN3VN_RS04950 (ACN3VN_04950) pilU 1071044..1072174 (-) 1131 WP_010894128.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN3VN_RS04955 (ACN3VN_04955) pilT 1072340..1073374 (-) 1035 WP_046419718.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN3VN_RS04960 (ACN3VN_04960) - 1074011..1074223 (+) 213 WP_046419721.1 hypothetical protein -
  ACN3VN_RS04965 (ACN3VN_04965) - 1074636..1075463 (+) 828 WP_171817080.1 glycosyltransferase family 2 protein -
  ACN3VN_RS04970 (ACN3VN_04970) - 1076127..1076951 (-) 825 WP_010894133.1 glycosyltransferase family 2 protein -
  ACN3VN_RS04975 (ACN3VN_04975) - 1076948..1077727 (-) 780 WP_023906627.1 beta-ketoacyl synthase chain length factor -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38527.22 Da        Isoelectric Point: 6.8843

>NTDB_id=983175 ACN3VN_RS04955 WP_046419718.1 1072340..1073374(-) (pilT) [Xylella fastidiosa strain CFBP8074]
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDVRRINIPAFDHKQVQSLIYDIMSDKQRRDYEEALETDFSFEIPSLSR
FRVNAFNQERGAGAVFRTIPNKVMTLDELGCLPVFRKLIEEPQGLILVTGPTGSGKSTTLAAMIDHINKNTHGHILTIED
PIEFVHTSQKCLINQREVHRDTHDFNKALSSALREDPDIILIGELRDLETIRLALTAAETGHLVFGTLHTNSAAKSINRI
IDVFPAAEKPMVRSMLSESLCGIISQMLLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLIKRNLITRQQAREYAKDKANF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=983175 ACN3VN_RS04955 WP_046419718.1 1072340..1073374(-) (pilT) [Xylella fastidiosa strain CFBP8074]
ATGGATATTGCTGAACTGTTAGCGTTTTCAGTCAAAAACAATGCTTCGGACCTGCACCTATCAGCAGGCCTGCCACCAAT
GATCAGGGTGGACGGCGATGTACGCCGTATCAATATCCCTGCATTCGACCATAAGCAGGTGCAATCGCTGATTTACGACA
TCATGTCGGACAAGCAACGACGCGACTACGAGGAAGCACTTGAGACGGATTTCTCGTTCGAAATTCCTTCACTCTCCCGC
TTCCGTGTCAATGCATTCAACCAAGAGCGTGGTGCTGGTGCCGTGTTTCGCACCATTCCGAATAAGGTGATGACATTGGA
CGAACTCGGCTGTTTGCCGGTGTTTCGCAAACTCATTGAAGAGCCGCAGGGATTGATTCTGGTGACTGGGCCGACTGGCT
CTGGAAAATCGACCACCCTCGCAGCCATGATCGACCATATCAACAAAAATACTCACGGGCATATTCTCACGATAGAGGAC
CCGATTGAGTTCGTTCACACCTCACAGAAGTGCTTGATTAACCAACGTGAGGTACATCGCGATACTCACGACTTTAACAA
AGCGCTCAGCTCAGCACTACGCGAAGATCCAGACATCATCCTGATTGGAGAATTGCGTGACCTGGAAACGATCCGTCTAG
CACTGACTGCGGCCGAAACTGGTCACTTGGTATTTGGCACCCTGCACACCAATTCAGCAGCGAAAAGTATTAACCGCATC
ATCGACGTGTTTCCCGCTGCTGAAAAACCGATGGTCCGCTCAATGCTTTCCGAATCGCTCTGCGGGATCATCTCGCAAAT
GTTGTTGAAAAAAGTTGGTGGTGGACGTACCGCAGCCTGGGAAATTATGGTTGGCACCCCCGCAATCCGAAATTTGATCC
GTGAGGATAAAGTCGCACAGATGTATTCATCCATCCAAACTGGCCAGCAATATGGCATGCAGACCCTTGACCAGCATTTA
CAGGATCTCATCAAACGCAACCTGATCACACGACAGCAAGCACGTGAATACGCCAAGGATAAAGCGAATTTTTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.872

100

0.759
  pilT Pseudomonas aeruginosa PAK

74.709

100

0.747
  pilT Acinetobacter nosocomialis M2

74.419

100

0.744
  pilT Acinetobacter baumannii D1279779

74.419

100

0.744
  pilT Acinetobacter baumannii strain A118

74.419

100

0.744
  pilT Acinetobacter baylyi ADP1

74.419

100

0.744
  pilT Legionella pneumophila strain Lp02

71.221

100

0.712
  pilT Legionella pneumophila strain ERS1305867

71.221

100

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.06

97.384

0.663
  pilT Vibrio cholerae strain A1552

68.06

97.384

0.663
  pilT Neisseria gonorrhoeae MS11

62.463

99.128

0.619
  pilT Neisseria meningitidis 8013

62.17

99.128

0.616
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.491
  pilU Pseudomonas stutzeri DSM 10701

40.698

100

0.407
  pilU Vibrio cholerae strain A1552

39.826

100

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398