Detailed information NTG0983174

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACN3VN_RS04950
• Genome accession: NZ_CP183396
• Coordinates: 1071044..1072174 (-)
• Length: 376 a.a.
• NCBI ID: WP_010894128.1
• Uniprot ID: Q9PCX2
• Organism: Xylella fastidiosa strain CFBP8074
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1066044..1077174

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACN3VN_RS04930 (ACN3VN_04930)	-	1066115..1066873 (+)	759	WP_010894122.1	LytR/AlgR family response regulator transcription factor	-
ACN3VN_RS04935 (ACN3VN_04935)	hemC	1066890..1067807 (+)	918	WP_046419711.1	hydroxymethylbilane synthase	-
ACN3VN_RS04940 (ACN3VN_04940)	-	1067861..1068670 (-)	810	WP_021358437.1	DUF481 domain-containing protein	-
ACN3VN_RS04945 (ACN3VN_04945)	-	1069514..1070527 (+)	1014	Protein_959	helix-turn-helix transcriptional regulator	-
ACN3VN_RS04950 (ACN3VN_04950)	pilU	1071044..1072174 (-)	1131	WP_010894128.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACN3VN_RS04955 (ACN3VN_04955)	pilT	1072340..1073374 (-)	1035	WP_046419718.1	type IV pilus twitching motility protein PilT	Machinery gene
ACN3VN_RS04960 (ACN3VN_04960)	-	1074011..1074223 (+)	213	WP_046419721.1	hypothetical protein	-
ACN3VN_RS04965 (ACN3VN_04965)	-	1074636..1075463 (+)	828	WP_171817080.1	glycosyltransferase family 2 protein	-
ACN3VN_RS04970 (ACN3VN_04970)	-	1076127..1076951 (-)	825	WP_010894133.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42476.67 Da
• Isoelectric Point: 6.5027

>NTDB_id=983174 ACN3VN_RS04950 WP_010894128.1 1071044..1072174(-) (pilU) [Xylella fastidiosa strain CFBP8074]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITN
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLTDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=983174 ACN3VN_RS04950 WP_010894128.1 1071044..1072174(-) (pilU) [Xylella fastidiosa strain CFBP8074]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAAGTCCATGAATGCAACTTCGCAATTGACATCACCAAC
ATTGGTCGCTTCCGCGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCAGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGACTGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACTACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCCAAAACTCTGAGTCAAGGATTCGATGGAGTAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-272)

3D structure

• AlphaFold DB: Q9PCX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	51.124	94.681	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399