Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAFX31_RS13280 Genome accession   NZ_CP151941
Coordinates   1143039..1144076 (-) Length   345 a.a.
NCBI ID   WP_061032415.1    Uniprot ID   A0A2N7HK89
Organism   Vibrio chagasii strain TBV018     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1138039..1149076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX31_RS13250 (AAFX31_13260) rsmE 1138739..1139470 (+) 732 WP_061032418.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAFX31_RS13255 (AAFX31_13265) gshB 1139480..1140430 (+) 951 WP_102424750.1 glutathione synthase -
  AAFX31_RS13260 (AAFX31_13270) - 1140539..1141102 (+) 564 WP_032498752.1 YqgE/AlgH family protein -
  AAFX31_RS13265 (AAFX31_13275) ruvX 1141163..1141585 (+) 423 WP_004738973.1 Holliday junction resolvase RuvX -
  AAFX31_RS13270 (AAFX31_13280) - 1141685..1141855 (+) 171 WP_155647308.1 hypothetical protein -
  AAFX31_RS13275 (AAFX31_13285) pilU 1141915..1143024 (-) 1110 WP_061032416.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAFX31_RS13280 (AAFX31_13290) pilT 1143039..1144076 (-) 1038 WP_061032415.1 type IV pilus twitching motility protein PilT Machinery gene
  AAFX31_RS13285 (AAFX31_13295) - 1144103..1144807 (+) 705 WP_061032414.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAFX31_RS13290 (AAFX31_13300) proC 1144889..1145707 (+) 819 WP_061032413.1 pyrroline-5-carboxylate reductase -
  AAFX31_RS13295 (AAFX31_13305) - 1145784..1146341 (+) 558 WP_017069592.1 YggT family protein -
  AAFX31_RS13300 (AAFX31_13310) yggU 1146371..1146661 (+) 291 WP_061032412.1 DUF167 family protein YggU -
  AAFX31_RS13305 (AAFX31_13315) - 1146690..1147121 (+) 432 WP_411062160.1 DUF4426 domain-containing protein -
  AAFX31_RS13310 (AAFX31_13320) - 1147170..1147769 (+) 600 WP_411062161.1 XTP/dITP diphosphatase -
  AAFX31_RS13315 (AAFX31_13325) hemW 1147769..1148956 (+) 1188 WP_105057607.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38213.76 Da        Isoelectric Point: 6.0507

>NTDB_id=981407 AAFX31_RS13280 WP_061032415.1 1143039..1144076(-) (pilT) [Vibrio chagasii strain TBV018]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLEQLGAPDIFEKIANFEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=981407 AAFX31_RS13280 WP_061032415.1 1143039..1144076(-) (pilT) [Vibrio chagasii strain TBV018]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTGCACCGTTTGGTTTTTGAGA
TCATGAGCGACTCGCAGCGCGGTGAATTTGAGGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTCGGTCGT
TTCCGTGTTAACGCTTTTAACCAAGCCCGTGGCTGCTCAGCTGTCTTTCGAACTATCCCTGTCGAGATTCCGACGTTAGA
ACAGTTAGGCGCTCCTGATATCTTTGAAAAGATTGCTAATTTCGAAAAGGGTCTAGTGTTAGTTACAGGCCCAACTGGTT
CTGGTAAATCGACTACTCTTGCGGCGATGGTGGATTACGTTAACCGTAACCATAATAAGCATATTCTCACCATAGAAGAC
CCGATTGAATTTGTTCACACCAACAATAAGTGCCTAGTGAACCAGCGTGAGGTTCATCGTGATACTCATAGCTTTAAAGC
GGCGCTGCGCAGTGCGTTACGTGAAGACCCTGATGTTATCCTTGTTGGTGAGCTTCGTGACCAAGAAACCATTAGTTTAG
CGCTCACGGCTGCAGAAACTGGTCACTTGGTTTTTGGTACCTTACATACCAGTTCTGCGGCAAAAACGATTGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGTTATCTGAATCATTACGCTCGGTGATTGCCCAGAA
GTTGTTAAAACGGGTTGGTGGTGGTCGTGTGGCTTGTCATGAAATAATGATGGCGACACCAGCGATCAGAAACTTGATTC
GTGAAGATAAGGTCGCTCAGATGTATTCGATCATTCAAACGGGTGCAGCACATGGCATGCAAACCATGGAGCAAAATGCG
AAGCAGCTGATGGCTCAAGGCTTAGTTGACTCGGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7HK89

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

71.988

96.232

0.693
  pilT Acinetobacter baumannii strain A118

71.988

96.232

0.693
  pilT Acinetobacter nosocomialis M2

71.687

96.232

0.69
  pilT Pseudomonas aeruginosa PAK

68.824

98.551

0.678
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.681

0.678
  pilT Legionella pneumophila strain Lp02

66.087

100

0.661
  pilT Legionella pneumophila strain ERS1305867

66.087

100

0.661
  pilT Neisseria meningitidis 8013

62.899

100

0.629
  pilT Neisseria gonorrhoeae MS11

62.609

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.317

96.812

0.4
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment