Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAFX31_RS13275 Genome accession   NZ_CP151941
Coordinates   1141915..1143024 (-) Length   369 a.a.
NCBI ID   WP_061032416.1    Uniprot ID   -
Organism   Vibrio chagasii strain TBV018     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1136915..1148024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX31_RS13240 (AAFX31_13250) - 1137204..1137704 (+) 501 WP_061032419.1 SprT family zinc-dependent metalloprotease -
  AAFX31_RS13245 (AAFX31_13255) - 1137854..1138585 (+) 732 WP_008223584.1 endonuclease -
  AAFX31_RS13250 (AAFX31_13260) rsmE 1138739..1139470 (+) 732 WP_061032418.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAFX31_RS13255 (AAFX31_13265) gshB 1139480..1140430 (+) 951 WP_102424750.1 glutathione synthase -
  AAFX31_RS13260 (AAFX31_13270) - 1140539..1141102 (+) 564 WP_032498752.1 YqgE/AlgH family protein -
  AAFX31_RS13265 (AAFX31_13275) ruvX 1141163..1141585 (+) 423 WP_004738973.1 Holliday junction resolvase RuvX -
  AAFX31_RS13270 (AAFX31_13280) - 1141685..1141855 (+) 171 WP_155647308.1 hypothetical protein -
  AAFX31_RS13275 (AAFX31_13285) pilU 1141915..1143024 (-) 1110 WP_061032416.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAFX31_RS13280 (AAFX31_13290) pilT 1143039..1144076 (-) 1038 WP_061032415.1 type IV pilus twitching motility protein PilT Machinery gene
  AAFX31_RS13285 (AAFX31_13295) - 1144103..1144807 (+) 705 WP_061032414.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAFX31_RS13290 (AAFX31_13300) proC 1144889..1145707 (+) 819 WP_061032413.1 pyrroline-5-carboxylate reductase -
  AAFX31_RS13295 (AAFX31_13305) - 1145784..1146341 (+) 558 WP_017069592.1 YggT family protein -
  AAFX31_RS13300 (AAFX31_13310) yggU 1146371..1146661 (+) 291 WP_061032412.1 DUF167 family protein YggU -
  AAFX31_RS13305 (AAFX31_13315) - 1146690..1147121 (+) 432 WP_411062160.1 DUF4426 domain-containing protein -
  AAFX31_RS13310 (AAFX31_13320) - 1147170..1147769 (+) 600 WP_411062161.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41353.22 Da        Isoelectric Point: 5.8713

>NTDB_id=981406 AAFX31_RS13275 WP_061032416.1 1141915..1143024(-) (pilU) [Vibrio chagasii strain TBV018]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEKLNSAQVAQLLDAMMDQERRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEVGMQTFDQALF
DLVVAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALSGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=981406 AAFX31_RS13275 WP_061032416.1 1141915..1143024(-) (pilU) [Vibrio chagasii strain TBV018]
ATGGAATTGAATCAAATCCTTGAGGGAATGCTTTCTCAAAAAGCGTCCGATCTTTACATCACGGTTGATGCGCCTGTTTT
GTTCCGTGTCGATGGTGAGCTGCGACCGCAAGGTGAGAAGCTGAATTCGGCGCAGGTCGCTCAGCTACTTGATGCGATGA
TGGATCAAGAACGACGTGATGAATATCAGCAAACGCGTGAGGCTAACTTTGCCATTGTACGTGATTTTGGTCGCTTTCGT
GTTAGTGCGTTCTTTCAGCGAGAGCTGCCTGGAGCAGTGATTCGACGCATCGAGACGAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTATTACAAGACCTCTCAATTGCCAAGCGTGGGCTTGTGCTGGTGGTTGGGGCTACGGGTTCTGGTA
AATCGACCTCGATGGCGGCGATGACAGGCTACCGCAATACCAATCGCTCGGGGCATATCTTGACGGTTGAAGACCCGATT
GAATTCGTCCACGAACATAAGAAATGCATAGTTACTCAGCGTGAGGTAGGGCTCGACACCGAGAGCTATGAAGTCGCCCT
TAAAAACTCGTTGCGCCAAGCGCCAGATATGATTTTGATTGGCGAAATCCGTAGCCGCGAAACCATGGAATATGCGATGA
CCTTTGCTGAGACGGGTCACTTGTGTATGGCAACTTTGCACGCGAATAATGCCAACCAAGCGTTAGAGCGTATTCTTCAT
TTGGTGCCTAAAGAACAGAAAGAACAGTTCCTGTTTGATCTGTCGATGAATTTGCGTGGTGTGATTGCTCAGCAGTTAAT
TCGAGATAAGAATGGCAGCGGGCGTCATGGTGTATTTGAAATTCTACTCAACAGCCCACGAGTGTCTGACTTGATTCGTC
GCGGTGAATTGCACGAGCTAAAAGCAACAATGGCAAAATCAAAAGAAGTCGGCATGCAAACCTTTGACCAAGCTTTGTTT
GATTTAGTTGTAGCGGGAAAGATTAGCGAAGAAGATGCGTTTCATAGTGCCGATTCGGCTAATGACTTACGTTTGATGCT
AAAAACCAGACGAGGTGATGATGACTATGGAACTGGAGCATTATCTGGCGTGAAAATTGATATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.783

99.729

0.846
  pilU Pseudomonas stutzeri DSM 10701

56.065

100

0.564
  pilU Acinetobacter baylyi ADP1

54.986

95.122

0.523
  pilT Acinetobacter baumannii strain A118

42.647

92.141

0.393
  pilT Acinetobacter baumannii D1279779

42.647

92.141

0.393
  pilT Acinetobacter nosocomialis M2

42.647

92.141

0.393
  pilT Legionella pneumophila strain ERS1305867

41.888

91.87

0.385
  pilT Legionella pneumophila strain Lp02

41.888

91.87

0.385
  pilT Pseudomonas aeruginosa PAK

42.09

90.786

0.382
  pilT Acinetobacter baylyi ADP1

42.47

89.973

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.901

87.805

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.515

0.369
  pilT Vibrio cholerae strain A1552

40.719

90.515

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.597

90.786

0.369
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363

Multiple sequence alignment