Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAGT95_RS04505 Genome accession   NZ_CP151919
Coordinates   958106..959140 (-) Length   344 a.a.
NCBI ID   WP_110601309.1    Uniprot ID   -
Organism   Salinicola lusitanus strain LLJ914     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 953106..964140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGT95_RS04485 (AAGT95_04485) - 954178..954402 (+) 225 WP_342595663.1 hypothetical protein -
  AAGT95_RS04490 (AAGT95_04490) tyrS 954507..955706 (-) 1200 WP_342595664.1 tyrosine--tRNA ligase -
  AAGT95_RS04495 (AAGT95_04495) - 955806..956921 (+) 1116 WP_342595665.1 anhydro-N-acetylmuramic acid kinase -
  AAGT95_RS04500 (AAGT95_04500) pilU 956938..958071 (-) 1134 WP_342595666.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGT95_RS04505 (AAGT95_04505) pilT 958106..959140 (-) 1035 WP_110601309.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGT95_RS04510 (AAGT95_04510) - 959180..959890 (+) 711 WP_110601310.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGT95_RS04515 (AAGT95_04515) proC 959930..960769 (+) 840 WP_342595667.1 pyrroline-5-carboxylate reductase -
  AAGT95_RS04520 (AAGT95_04520) - 960803..961393 (+) 591 WP_110601313.1 YggT family protein -
  AAGT95_RS04525 (AAGT95_04525) - 961545..962702 (+) 1158 WP_110601315.1 homoserine O-acetyltransferase -
  AAGT95_RS04530 (AAGT95_04530) metW 962702..963298 (+) 597 WP_157958431.1 methionine biosynthesis protein MetW -
  AAGT95_RS04535 (AAGT95_04535) sfsA 963295..964050 (-) 756 WP_342595668.1 DNA/RNA nuclease SfsA -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37639.96 Da        Isoelectric Point: 6.4195

>NTDB_id=981165 AAGT95_RS04505 WP_110601309.1 958106..959140(-) (pilT) [Salinicola lusitanus strain LLJ914]
MDITELLAFSAKHDASDLHLSAGLPPMIRVDGDMRRLNVPALDDSQVRRLIYDVMNDRQRRDFEATLETDFSFEVTGVSR
FRVNAFTQGRGAGAVFRTIPSNVLTLDDLGLGDVFRRLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINSNRQEHILTIED
PIEFVHESKRCLINQREVHRDTKGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKGMVRSMLSESLQAVISQTLLKREAGGRVAAHEILVATSAIRNLIREGKVAQMYSAIQTGGSLGMQTLDAAL
SQLVADGVVTRQEAHAKSRAGLDQ

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=981165 AAGT95_RS04505 WP_110601309.1 958106..959140(-) (pilT) [Salinicola lusitanus strain LLJ914]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAGCACGACGCTTCGGACCTGCACCTGTCGGCGGGGCTGCCGCCCAT
GATACGGGTCGATGGGGATATGCGTCGCCTCAATGTGCCCGCGCTCGACGACAGCCAGGTGCGCCGACTGATCTATGATG
TGATGAACGACCGTCAGCGTCGGGATTTCGAAGCTACGCTCGAGACCGACTTCTCGTTCGAAGTTACCGGCGTGTCTCGC
TTCCGTGTCAATGCTTTCACGCAGGGACGCGGTGCCGGCGCGGTCTTTCGTACCATTCCCAGCAATGTGCTGACGCTCGA
CGACCTCGGGTTGGGCGACGTCTTCCGGCGTCTGTCGATGTTGCCGCGAGGCCTGGTGCTGGTCACGGGGCCGACCGGCA
GTGGCAAGTCGACGACGCTGGCGGCGATGATCGACTACATCAACAGCAACCGTCAGGAACACATCCTGACCATCGAGGAT
CCGATCGAATTCGTGCACGAAAGCAAGCGTTGCCTGATCAATCAGCGTGAAGTGCATCGCGACACCAAGGGCTTCGCCCC
AGCCCTGCGCTCGGCCCTGCGCGAAGATCCCGATGTCATCCTGGTCGGCGAGCTCAGGGACCTGGAGACCATTCGGCTCG
CCTTGACGGCTGCGGAGACCGGCCATCTGGTATTCGGCACCCTGCACACCACGTCGGCGGCCAAGACCATCGACCGCATC
ATCGATGTCTTTCCCGGTGACGAGAAGGGCATGGTGCGCTCGATGCTGTCCGAATCGCTGCAGGCGGTGATCTCACAGAC
GCTGCTCAAGCGCGAGGCCGGTGGGCGTGTGGCGGCTCATGAGATTCTGGTGGCGACATCGGCGATTCGCAATCTGATAC
GGGAAGGCAAAGTGGCCCAGATGTACTCGGCGATTCAGACCGGCGGTAGCCTGGGGATGCAGACGCTGGACGCGGCGCTT
TCCCAGTTGGTGGCCGACGGCGTGGTGACCCGCCAGGAAGCTCATGCCAAATCGCGCGCCGGACTCGACCAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.466

98.547

0.773
  pilT Pseudomonas aeruginosa PAK

78.466

98.547

0.773
  pilT Acinetobacter baumannii D1279779

76.401

98.547

0.753
  pilT Acinetobacter baumannii strain A118

76.401

98.547

0.753
  pilT Acinetobacter baylyi ADP1

76.176

98.837

0.753
  pilT Acinetobacter nosocomialis M2

76.106

98.547

0.75
  pilT Legionella pneumophila strain Lp02

70.448

97.384

0.686
  pilT Legionella pneumophila strain ERS1305867

70.448

97.384

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.965

0.683
  pilT Vibrio cholerae strain A1552

69.733

97.965

0.683
  pilT Neisseria meningitidis 8013

65.294

98.837

0.645
  pilT Neisseria gonorrhoeae MS11

65

98.837

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.381

96.221

0.523
  pilU Pseudomonas stutzeri DSM 10701

43.188

100

0.433
  pilU Vibrio cholerae strain A1552

42.985

97.384

0.419
  pilU Acinetobacter baylyi ADP1

41.618

100

0.419

Multiple sequence alignment