Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAGT95_RS04500 Genome accession   NZ_CP151919
Coordinates   956938..958071 (-) Length   377 a.a.
NCBI ID   WP_342595666.1    Uniprot ID   -
Organism   Salinicola lusitanus strain LLJ914     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 951938..963071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGT95_RS04485 (AAGT95_04485) - 954178..954402 (+) 225 WP_342595663.1 hypothetical protein -
  AAGT95_RS04490 (AAGT95_04490) tyrS 954507..955706 (-) 1200 WP_342595664.1 tyrosine--tRNA ligase -
  AAGT95_RS04495 (AAGT95_04495) - 955806..956921 (+) 1116 WP_342595665.1 anhydro-N-acetylmuramic acid kinase -
  AAGT95_RS04500 (AAGT95_04500) pilU 956938..958071 (-) 1134 WP_342595666.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGT95_RS04505 (AAGT95_04505) pilT 958106..959140 (-) 1035 WP_110601309.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGT95_RS04510 (AAGT95_04510) - 959180..959890 (+) 711 WP_110601310.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGT95_RS04515 (AAGT95_04515) proC 959930..960769 (+) 840 WP_342595667.1 pyrroline-5-carboxylate reductase -
  AAGT95_RS04520 (AAGT95_04520) - 960803..961393 (+) 591 WP_110601313.1 YggT family protein -
  AAGT95_RS04525 (AAGT95_04525) - 961545..962702 (+) 1158 WP_110601315.1 homoserine O-acetyltransferase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41587.62 Da        Isoelectric Point: 5.4816

>NTDB_id=981164 AAGT95_RS04500 WP_342595666.1 956938..958071(-) (pilU) [Salinicola lusitanus strain LLJ914]
MTPSQWFEQLLDIMLAKQASDLLISTGAPPCLKLDGKLVALGDVPLQPDQVRELVAIALPRGRQEAFEVDHEANFALSLS
GKGRFRVSAFYQRSQPAMVVRRIAFDIPDLESLGLPEVTATLASIRRGLVLVVGGTGSGKSTTLASMIQHRNRTVGGHII
CIEDPIEYMHPHQRGIINQREVGIDTESYEVALKNALRQAPDVIMIGEIRSRDTMELALSFAETGHLCLATLHANSADQA
IERILSLFPPSRQEQIGMDLSLNLRAVVAQRLLPRQDGAGRCVATEVLLKSPLISELIRRGEVAELKDLMRRSREQGMQT
FDQALYELFRRGDISESLALAHADSANDLRMLLKYGERRDAGDSSDDGAKFSLIDDE

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=981164 AAGT95_RS04500 WP_342595666.1 956938..958071(-) (pilU) [Salinicola lusitanus strain LLJ914]
ATGACCCCCTCTCAATGGTTTGAGCAGCTTCTCGATATCATGCTGGCCAAGCAGGCTTCGGATCTGCTGATCTCTACCGG
GGCGCCGCCCTGCCTGAAGCTGGATGGAAAACTGGTGGCCCTGGGCGATGTCCCCCTGCAGCCGGACCAGGTGCGCGAGC
TGGTGGCCATCGCTTTGCCCCGGGGGCGGCAAGAGGCTTTCGAGGTGGATCACGAGGCCAATTTCGCCCTCAGCCTCAGC
GGCAAGGGGCGTTTCCGTGTCAGCGCCTTCTACCAGCGCAGCCAACCGGCGATGGTCGTTCGCCGGATCGCGTTCGACAT
TCCCGATCTCGAATCGCTTGGCTTGCCGGAAGTGACGGCGACATTGGCCTCCATTCGGCGTGGACTGGTGCTGGTGGTGG
GTGGGACCGGCAGCGGCAAGTCCACCACGCTGGCCTCGATGATCCAGCACCGCAATCGCACGGTTGGCGGTCATATCATC
TGTATCGAAGACCCGATCGAATACATGCATCCGCACCAGCGCGGCATCATCAACCAGCGTGAAGTGGGGATCGACACCGA
ATCCTACGAGGTAGCGCTGAAGAATGCCCTGCGCCAGGCGCCGGATGTCATCATGATCGGCGAGATACGCAGCCGGGATA
CCATGGAACTGGCGCTCAGCTTTGCCGAAACCGGCCATCTCTGCCTGGCCACGCTGCACGCCAACAGCGCCGATCAGGCG
ATCGAGCGGATTCTCAGTCTGTTTCCGCCGTCACGCCAGGAGCAGATAGGGATGGATCTGTCGCTCAATCTGCGTGCCGT
GGTCGCTCAGCGACTGTTGCCGCGTCAGGATGGCGCCGGTCGCTGTGTGGCGACCGAGGTGCTGCTCAAGTCTCCCCTGA
TCAGCGAACTGATCCGTCGTGGCGAGGTCGCCGAGCTGAAGGATCTGATGCGCCGCTCCCGGGAGCAGGGCATGCAGACC
TTCGACCAGGCGCTCTACGAGCTGTTCCGGCGAGGGGACATCAGCGAGAGTCTGGCGCTGGCGCATGCCGATTCGGCCAA
TGACCTGCGCATGCTGCTCAAGTATGGCGAGCGTCGGGACGCCGGTGACAGCAGCGATGATGGCGCGAAGTTCTCGCTGA
TCGACGATGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

56.948

97.347

0.554
  pilU Pseudomonas stutzeri DSM 10701

55.764

98.939

0.552
  pilU Acinetobacter baylyi ADP1

54.108

93.634

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.356

90.981

0.422
  pilT Legionella pneumophila strain ERS1305867

42.169

88.064

0.371
  pilT Legionella pneumophila strain Lp02

42.169

88.064

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.901

85.942

0.369
  pilT Vibrio cholerae strain A1552

42.901

85.942

0.369

Multiple sequence alignment