Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAGA51_RS13185 Genome accession   NZ_CP151842
Coordinates   2866753..2867790 (-) Length   345 a.a.
NCBI ID   WP_042489193.1    Uniprot ID   -
Organism   Vibrio diazotrophicus strain ATCC 33466     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2861753..2872790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGA51_RS13155 - 2861805..2862506 (+) 702 WP_042489178.1 endonuclease -
  AAGA51_RS13160 rsmE 2862793..2863524 (+) 732 WP_042489181.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAGA51_RS13165 gshB 2863537..2864493 (+) 957 WP_042489183.1 glutathione synthase -
  AAGA51_RS13170 - 2864520..2865083 (+) 564 WP_042489186.1 YqgE/AlgH family protein -
  AAGA51_RS13175 ruvX 2865156..2865581 (+) 426 WP_042489189.1 Holliday junction resolvase RuvX -
  AAGA51_RS13180 pilU 2865635..2866741 (-) 1107 WP_042489190.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGA51_RS13185 pilT 2866753..2867790 (-) 1038 WP_042489193.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGA51_RS13190 - 2867835..2868539 (+) 705 WP_042489196.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGA51_RS13195 proC 2868635..2869453 (+) 819 WP_042489198.1 pyrroline-5-carboxylate reductase -
  AAGA51_RS13200 - 2869597..2870154 (+) 558 WP_042489200.1 YggT family protein -
  AAGA51_RS13205 yggU 2870154..2870486 (+) 333 WP_042489203.1 DUF167 family protein YggU -
  AAGA51_RS13210 - 2870510..2870941 (+) 432 WP_042489206.1 DUF4426 domain-containing protein -
  AAGA51_RS13215 - 2870966..2871565 (+) 600 WP_042489209.1 XTP/dITP diphosphatase -
  AAGA51_RS13220 hemW 2871565..2872737 (+) 1173 WP_042489211.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38197.67 Da        Isoelectric Point: 6.3875

>NTDB_id=981100 AAGA51_RS13185 WP_042489193.1 2866753..2867790(-) (pilT) [Vibrio diazotrophicus strain ATCC 33466]
MNIAELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLVFEVMNDAQRSEFEEKLEVDFSFELPDVGR
FRVNAFHQARGCSAVFRTIPTVIPTLDQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINKNHNKHILTIED
PIEFVHSNDKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGNDKDMVRSMLSESLRAVIAQKLLKRTGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMDQNS
KQLIAQGLVDPAEVQKKIESEISTF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=981100 AAGA51_RS13185 WP_042489193.1 2866753..2867790(-) (pilT) [Vibrio diazotrophicus strain ATCC 33466]
ATGAATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCAGGTGTTCCGCCTAT
GGTACGTATAGATGGCGAAGTAAGAAAGCTTGGCGTACCTGCTTTTACCCATGCAGATGTTCATCGTCTAGTGTTTGAAG
TGATGAATGATGCTCAGCGCAGTGAGTTTGAAGAAAAACTCGAAGTGGATTTTTCTTTTGAACTGCCGGATGTAGGGCGC
TTTCGTGTGAATGCATTCCACCAGGCACGCGGCTGTTCTGCGGTATTTCGAACCATCCCGACAGTCATTCCTACTCTTGA
CCAGTTAGATGCGCCAGAGATTTTCAGCAAAATTGCCAACTATGAGAAAGGTCTGGTTCTGGTGACTGGCCCAACGGGTT
CTGGTAAATCCACCACTTTGGCTGCGATGGTTGATTACATTAACAAGAATCACAACAAGCATATTCTTACCATTGAAGAT
CCAATCGAATTCGTTCATAGCAACGATAAGTGTTTGATCAACCAACGCGAGGTTCACCGCGATACGCACAGTTTCAAGAA
TGCGCTGCGTTCGGCACTGCGTGAAGACCCAGATGTCATTCTGGTCGGTGAGTTGCGTGACCAAGAGACCATTAGCCTAG
CATTAACTGCGGCGGAAACAGGACACCTTGTTTTCGGTACGCTGCATACCAGTTCGGCAGCAAAAACCATTGACCGTATT
ATTGATGTGTTTCCGGGCAACGATAAAGATATGGTGCGTTCTATGTTGTCTGAATCCTTACGCGCGGTGATCGCACAGAA
ACTGCTAAAACGTACTGGCGGAGGTCGAGTTGCTTGTCATGAGATCATGTTAGCGACACCTGCAATTCGTAACTTGATCC
GTGAAGATAAAGTGGCTCAGATGTATTCGATCATCCAAACCGGTGCTGCTCACGGAATGCAGACCATGGATCAAAACTCC
AAACAGCTGATTGCTCAAGGTTTAGTGGATCCGGCTGAAGTACAGAAGAAGATTGAGTCTGAAATCTCTACCTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.174

100

0.922
  pilT Vibrio cholerae strain A1552

92.174

100

0.922
  pilT Acinetobacter baumannii D1279779

72.205

95.942

0.693
  pilT Acinetobacter baumannii strain A118

72.205

95.942

0.693
  pilT Acinetobacter nosocomialis M2

71.903

95.942

0.69
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

67.359

97.681

0.658
  pilT Legionella pneumophila strain Lp02

66.565

95.362

0.635
  pilT Legionella pneumophila strain ERS1305867

66.565

95.362

0.635
  pilT Neisseria meningitidis 8013

63.112

100

0.635
  pilT Neisseria gonorrhoeae MS11

62.824

100

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.774

92.174

0.496
  pilU Vibrio cholerae strain A1552

44

94.203

0.414
  pilU Pseudomonas stutzeri DSM 10701

42.683

95.072

0.406
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374

Multiple sequence alignment