Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAGA51_RS13180 Genome accession   NZ_CP151842
Coordinates   2865635..2866741 (-) Length   368 a.a.
NCBI ID   WP_042489190.1    Uniprot ID   -
Organism   Vibrio diazotrophicus strain ATCC 33466     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2860635..2871741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGA51_RS13150 - 2861165..2861668 (+) 504 WP_042489176.1 SprT family zinc-dependent metalloprotease -
  AAGA51_RS13155 - 2861805..2862506 (+) 702 WP_042489178.1 endonuclease -
  AAGA51_RS13160 rsmE 2862793..2863524 (+) 732 WP_042489181.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAGA51_RS13165 gshB 2863537..2864493 (+) 957 WP_042489183.1 glutathione synthase -
  AAGA51_RS13170 - 2864520..2865083 (+) 564 WP_042489186.1 YqgE/AlgH family protein -
  AAGA51_RS13175 ruvX 2865156..2865581 (+) 426 WP_042489189.1 Holliday junction resolvase RuvX -
  AAGA51_RS13180 pilU 2865635..2866741 (-) 1107 WP_042489190.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGA51_RS13185 pilT 2866753..2867790 (-) 1038 WP_042489193.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGA51_RS13190 - 2867835..2868539 (+) 705 WP_042489196.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGA51_RS13195 proC 2868635..2869453 (+) 819 WP_042489198.1 pyrroline-5-carboxylate reductase -
  AAGA51_RS13200 - 2869597..2870154 (+) 558 WP_042489200.1 YggT family protein -
  AAGA51_RS13205 yggU 2870154..2870486 (+) 333 WP_042489203.1 DUF167 family protein YggU -
  AAGA51_RS13210 - 2870510..2870941 (+) 432 WP_042489206.1 DUF4426 domain-containing protein -
  AAGA51_RS13215 - 2870966..2871565 (+) 600 WP_042489209.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41422.46 Da        Isoelectric Point: 6.5070

>NTDB_id=981099 AAGA51_RS13180 WP_042489190.1 2865635..2866741(-) (pilU) [Vibrio diazotrophicus strain ATCC 33466]
MELNHYLEAMTSLKASDLYITVGAPVLLRVDGEMRPQGEKLSQQEVLSLLHEMMDEERKTEFNASHEANFAVVRGNGRFR
VSAFFQRELPGAVIRRIETQIPTFEELHLPVVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNRHRSGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVGQQLIRDKNGKGRHGVFEILLNSPRVSDLIRKGELHELKSTMAKSKEIGMQTFDQALY
QLLVEDKITEQDAMHSADSANDLRMMLKTQRGDTPSSSSLANVKIDMN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=981099 AAGA51_RS13180 WP_042489190.1 2865635..2866741(-) (pilU) [Vibrio diazotrophicus strain ATCC 33466]
ATGGAATTGAATCACTACCTTGAAGCGATGACCTCGCTTAAAGCGTCGGACTTATATATTACTGTCGGTGCACCGGTATT
GCTGCGAGTGGATGGTGAGATGCGTCCGCAAGGGGAGAAGCTTTCACAGCAAGAGGTGCTTTCTTTATTACATGAAATGA
TGGATGAAGAACGTAAAACAGAATTCAACGCCTCACACGAAGCTAACTTTGCGGTTGTTAGGGGCAATGGGCGTTTCAGG
GTGAGTGCTTTTTTCCAACGTGAGTTACCGGGCGCTGTGATTCGACGTATCGAAACGCAAATTCCGACATTTGAAGAATT
GCACTTGCCTGTGGTTTTGCAAGATCTTGCGATTGCAAAGCGAGGCTTAGTGCTGGTCGTGGGAGCAACGGGTTCGGGTA
AATCCACCACTATGGCAGCAATGACGGGATATCGTAATCGTCATCGCAGTGGACATATCTTAACGGTTGAAGATCCGATT
GAGTTCGTACACGAACATCAAAAATGCATCGTCACTCAGCGAGAAGTGGGTTTAGATACCGAAAGTTACGAAGTCGCCCT
TAAGAACTCTCTTCGCCAAGCGCCAGACATGATTTTGATTGGCGAAATCCGCAGTCGTGAGACCATGGAATATGCAATGA
CGTTTGCTGAAACAGGGCATCTGTGTATGGCAACATTGCATGCTAACAATGCCAACCAAGCGCTAGAGCGTATTCTTCAC
CTTGTGCCTAAAGAGCAAAAAGAGCAGTTCTTGTTTGATTTGTCGATGAACTTGCGTGGCGTGGTGGGGCAGCAGCTGAT
TCGTGATAAAAATGGTAAAGGTCGCCACGGTGTGTTTGAGATCTTACTCAATTCACCTCGCGTTTCAGATTTGATTCGCA
AAGGAGAGTTACACGAGTTGAAATCGACGATGGCTAAATCGAAAGAGATTGGTATGCAGACGTTTGATCAAGCGTTGTAT
CAGTTACTAGTGGAAGACAAGATCACTGAACAAGATGCAATGCACAGCGCCGATTCTGCCAATGATTTGCGCATGATGTT
AAAAACTCAGCGTGGAGACACTCCGAGTTCAAGTTCACTGGCTAATGTGAAAATCGATATGAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.379

99.728

0.832
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

53.561

95.38

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Vibrio cholerae strain A1552

41.617

90.761

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

90.761

0.378
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Acinetobacter baumannii strain A118

40.413

92.12

0.372
  pilT Acinetobacter baumannii D1279779

40.413

92.12

0.372
  pilT Acinetobacter nosocomialis M2

40.413

92.12

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Neisseria gonorrhoeae MS11

38.551

93.75

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361

Multiple sequence alignment