Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAFF32_RS18205 Genome accession   NZ_CP151802
Coordinates   3919141..3920178 (-) Length   345 a.a.
NCBI ID   WP_216963531.1    Uniprot ID   A0AAP2HBP0
Organism   Lysobacter sp. FW306-1B-D06B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3914141..3925178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFF32_RS18185 (AAFF32_18185) - 3914537..3915106 (+) 570 WP_216963509.1 DNA-3-methyladenine glycosylase I -
  AAFF32_RS18190 (AAFF32_18190) - 3915175..3916287 (+) 1113 WP_342315947.1 DUF1254 domain-containing protein -
  AAFF32_RS18195 (AAFF32_18195) - 3916328..3917779 (+) 1452 WP_342315948.1 DUF1254 domain-containing protein -
  AAFF32_RS18200 (AAFF32_18200) pilU 3917841..3919010 (-) 1170 WP_216963529.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAFF32_RS18205 (AAFF32_18205) pilT 3919141..3920178 (-) 1038 WP_216963531.1 type IV pilus twitching motility protein PilT Machinery gene
  AAFF32_RS18210 (AAFF32_18210) - 3920326..3920985 (+) 660 WP_216964291.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAFF32_RS18215 (AAFF32_18215) proC 3921042..3921893 (+) 852 WP_216964293.1 pyrroline-5-carboxylate reductase -
  AAFF32_RS18220 (AAFF32_18220) - 3921907..3922383 (+) 477 WP_342315949.1 DUF4426 domain-containing protein -
  AAFF32_RS18225 (AAFF32_18225) - 3922620..3923420 (-) 801 WP_342315950.1 cobalamin-binding protein -
  AAFF32_RS18230 (AAFF32_18230) pdxH 3923524..3924126 (-) 603 WP_342315951.1 pyridoxamine 5'-phosphate oxidase -
  AAFF32_RS18235 (AAFF32_18235) - 3924144..3924950 (+) 807 WP_342315952.1 kinase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38518.27 Da        Isoelectric Point: 6.5777

>NTDB_id=980941 AAFF32_RS18205 WP_216963531.1 3919141..3920178(-) (pilT) [Lysobacter sp. FW306-1B-D06B]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLSLDDLGCPPIFRELIDQPQGLILVTGPTGSGKSTTLAAMLDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQNYGMMTLDQHL
QDLVKRGLILRPQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=980941 AAFF32_RS18205 WP_216963531.1 3919141..3920178(-) (pilT) [Lysobacter sp. FW306-1B-D06B]
ATGGATATCGCCGAACTTCTGGCGTTCTCAGTCAAGAACAAAGCTTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCCAT
GATCCGCGTGGACGGCGACGTGCGACGCATCAACATCCCGGCACTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAGGAATTCCTCGAAGTCGACTTCTCGTTCGAGATTCCCGGCCTGGCCCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACGATTCCCTCGGAAGTGCTGTCGCTGGA
CGACCTGGGCTGCCCGCCGATCTTCCGCGAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACGGGTT
CGGGCAAGTCGACCACGCTGGCGGCAATGCTCGATCACATCAACAAGAACGAATACGGCCACATCCTCTCCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
AGCGCTGCGCTCGGCACTGCGCGAAGACCCCGACTACATCCTCGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCCTCGGCCGCCAAGACGGTGGACCGCATC
ATCGACGTGTTCCCCGCCGGTGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTCATCTCGCAGGC
GCTGTTGAAGAAGGTCGGCGGCGGTCGTACCGCGGCGTGGGAAATCATGGTCGGCATCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGAACTACGGAATGATGACCCTCGACCAGCACCTG
CAGGACCTCGTCAAGCGCGGCCTGATCCTGCGTCCGCAGGCGAAGGAATACGCGAAGGACAAGCGGTTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.605

95.362

0.664
  pilT Vibrio cholerae strain A1552

69.605

95.362

0.664
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

65.103

98.841

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

100

0.487
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.303

95.652

0.386
  pilU Vibrio cholerae strain A1552

40

94.203

0.377

Multiple sequence alignment