Detailed information NTG0980940

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAFF32_RS18200
• Genome accession: NZ_CP151802
• Coordinates: 3917841..3919010 (-)
• Length: 389 a.a.
• NCBI ID: WP_216963529.1
• Uniprot ID: A0AAP2HAZ8
• Organism: Lysobacter sp. FW306-1B-D06B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3912841..3924010

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAFF32_RS18185 (AAFF32_18185)	-	3914537..3915106 (+)	570	WP_216963509.1	DNA-3-methyladenine glycosylase I	-
AAFF32_RS18190 (AAFF32_18190)	-	3915175..3916287 (+)	1113	WP_342315947.1	DUF1254 domain-containing protein	-
AAFF32_RS18195 (AAFF32_18195)	-	3916328..3917779 (+)	1452	WP_342315948.1	DUF1254 domain-containing protein	-
AAFF32_RS18200 (AAFF32_18200)	pilU	3917841..3919010 (-)	1170	WP_216963529.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AAFF32_RS18205 (AAFF32_18205)	pilT	3919141..3920178 (-)	1038	WP_216963531.1	type IV pilus twitching motility protein PilT	Machinery gene
AAFF32_RS18210 (AAFF32_18210)	-	3920326..3920985 (+)	660	WP_216964291.1	YggS family pyridoxal phosphate-dependent enzyme	-
AAFF32_RS18215 (AAFF32_18215)	proC	3921042..3921893 (+)	852	WP_216964293.1	pyrroline-5-carboxylate reductase	-
AAFF32_RS18220 (AAFF32_18220)	-	3921907..3922383 (+)	477	WP_342315949.1	DUF4426 domain-containing protein	-
AAFF32_RS18225 (AAFF32_18225)	-	3922620..3923420 (-)	801	WP_342315950.1	cobalamin-binding protein	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42957.45 Da
• Isoelectric Point: 6.8287

>NTDB_id=980940 AAFF32_RS18200 WP_216963529.1 3917841..3919010(-) (pilU) [Lysobacter sp. FW306-1B-D06B]
MNTSTNGTASAATGGSIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKIAPITQNPLTPQQSRDMVLNVMNPQQREEFE
KTHECNFAIGVAGVGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMI
GYRNLNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLV
LCTLHANNANQAMDRIINFFPEDRRQQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKLKE
LMKESVQLGMKTFDQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDAKTLAAGLDGVEVAEVR

Nucleotide

• Length: 1170 bp

>NTDB_id=980940 AAFF32_RS18200 WP_216963529.1 3917841..3919010(-) (pilU) [Lysobacter sp. FW306-1B-D06B]
ATGAACACCAGCACCAACGGCACCGCGTCCGCCGCCACCGGCGGCAGCATCGACTTCACCTCGTTCCTGAAGCTGATGGC
GCATCAGAAGGCTTCGGACCTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTCCACGGCAAGATCGCGCCGATCA
CGCAGAACCCGCTGACGCCGCAGCAGTCGCGCGACATGGTGCTCAACGTGATGAACCCGCAGCAGCGCGAGGAGTTCGAG
AAGACGCACGAGTGCAACTTCGCCATCGGCGTGGCCGGTGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCA
GGTCGGCATGGTCCTGCGTCGCATCGAGACCAAGATCCCGACGGTCGAAGAACTCAACCTGCCGCCGGTGATCAAGACGC
TGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCGACGGGCACGGGTAAGTCGACCTCGCTCGCGGCGATGATC
GGCTACCGCAACCTCAATTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTG
CATCATCACGCAGCGCGAGGTCGGCATCGACACCGATAGCTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCGG
ACGTGATCATGATCGGCGAGGTGCGCACGCGCGAGGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGTCACCTGGTG
CTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCCAGCA
GCTGCTGATGGACTTGTCCTTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGATCCCGACGCCGGACGGCAAGGGCCGTC
GCGTGGCGATGGAAATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAG
CTGATGAAGGAATCCGTGCAGCTGGGCATGAAGACCTTCGACCAGGCCCTGTTCGAGCTGTACCAGGCCGGCGAGATCTC
TTACGAGGACGCACTGCGCTACGCCGACTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCGA
AGACGCTGGCCGCGGGTCTGGACGGCGTGGAAGTGGCGGAAGTGCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (131-285)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	68.665	94.344	0.648
pilU	Acinetobacter baylyi ADP1	64.426	91.774	0.591
pilU	Vibrio cholerae strain A1552	53.652	91.517	0.491
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.351	95.116	0.393