Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AADH33_RS11340 Genome accession   NZ_CP151283
Coordinates   2742442..2743497 (+) Length   351 a.a.
NCBI ID   WP_075101771.1    Uniprot ID   A0AAW4J017
Organism   Psychrobacter sp. KFRI-CH2-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2737442..2748497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADH33_RS11325 (AADH33_11315) - 2738174..2739865 (+) 1692 WP_348548938.1 NAD+ synthase -
  AADH33_RS11330 (AADH33_11320) - 2740086..2741360 (+) 1275 WP_348548939.1 hemolysin family protein -
  AADH33_RS11335 (AADH33_11325) - 2741395..2742090 (-) 696 WP_348548940.1 YggS family pyridoxal phosphate-dependent enzyme -
  AADH33_RS11340 (AADH33_11330) pilT 2742442..2743497 (+) 1056 WP_075101771.1 type IV pilus twitching motility protein PilT Machinery gene
  AADH33_RS11345 (AADH33_11335) pilU 2743622..2744743 (+) 1122 WP_075101772.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AADH33_RS11350 (AADH33_11340) fur 2744899..2745333 (-) 435 WP_075101773.1 ferric iron uptake transcriptional regulator -
  AADH33_RS11355 (AADH33_11345) bamE 2745696..2746115 (+) 420 WP_083608584.1 outer membrane protein assembly factor BamE -
  AADH33_RS11360 (AADH33_11350) - 2746135..2746467 (-) 333 WP_256713751.1 RnfH family protein -
  AADH33_RS11365 (AADH33_11355) - 2746455..2747597 (-) 1143 WP_348548941.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39078.96 Da        Isoelectric Point: 6.8441

>NTDB_id=976080 AADH33_RS11340 WP_075101771.1 2742442..2743497(+) (pilT) [Psychrobacter sp. KFRI-CH2-11]
MAEKNTLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPAMTHQAVHQLIYDIMNDKQRADFEEFFETDFSFE
IPNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMSDVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINESRKEH
ILTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQAGGRIAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGAGDGMI
TLDQTLKNLVAKGTISKDVARPYAKQPETFL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=976080 AADH33_RS11340 WP_075101771.1 2742442..2743497(+) (pilT) [Psychrobacter sp. KFRI-CH2-11]
ATGGCTGAAAAAAACACCTTAAGCATCGAAGACTTGTTGCGTTTTACGGTTAAACATAAAGCATCTGATTTACATATTTC
AGCCGGTATGCCCGCCATGATTCGTGTCGATGGCGAGATGACGCGCATCAATATGCCAGCGATGACGCATCAAGCGGTGC
ATCAGCTGATTTATGACATCATGAATGACAAGCAGCGCGCCGATTTTGAGGAGTTTTTTGAGACGGACTTTTCTTTTGAG
ATTCCCAATCTTGCCCGCTTTCGGGTAAACGCCTTTAATCAAAACCGCGGCGCAGGTGCTGTGTTTCGTACCATTCCCTC
TAAGGTCCTAACGATGGAAGATTTGGGCATGAGCGATGTGTTCAAACGCGTGTCAGACTTCAAACGCGGCGTGGTGCTGG
TGACTGGCCCGACCGGCTCTGGTAAATCCACGACACTGGCAGCCATGATTGATTATATCAATGAGAGCCGCAAAGAGCAT
ATTTTGACCATCGAAGACCCGATCGAATTTGTCCACGAATCGAAAAAAAGCCTTATCAACCAGCGCGAAGTACACCGCGA
CACCTTGGGGTTTGAGCCAGCCCTACGCTCGGCACTGCGTGAGGATCCCGACGTTATTTTGGTGGGTGAGCTACGCGACC
TTGAGACCATTCGCTTGGCACTAACGGCAGCTGAGACCGGTCACTTAGTCTTTGGCACCTTGCACACCAGCTCAGCGGCC
AAAACCATTGACCGTGTGATTGATGTGTTTCCTGCCGCCGAAAAAGACATGATTCGCGCCATGCTCTCAGAGTCGCTACA
GGCAGTCATCTCACAGACCTTGCTGCGCCGTCAAGCAGGTGGCCGTATCGCCGCCCACGAGATCATGCTAGGTACGCCGG
CCATCCGTAACCTCATTCGCGAAAACAAAATCGCCCAGATGTATTCGGCGATTCAAACAGGCGCAGGCGACGGTATGATC
ACCCTTGACCAGACTTTAAAAAACCTCGTGGCCAAAGGCACCATCAGCAAAGACGTGGCGCGCCCTTATGCCAAGCAGCC
TGAGACTTTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.42

98.291

0.781
  pilT Acinetobacter baumannii strain A118

79.42

98.291

0.781
  pilT Acinetobacter nosocomialis M2

79.13

98.291

0.778
  pilT Pseudomonas aeruginosa PAK

77.616

98.006

0.761
  pilT Pseudomonas stutzeri DSM 10701

77.326

98.006

0.758
  pilT Acinetobacter baylyi ADP1

76.453

98.006

0.749
  pilT Legionella pneumophila strain Lp02

71.802

98.006

0.704
  pilT Legionella pneumophila strain ERS1305867

71.802

98.006

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.781

93.732

0.635
  pilT Vibrio cholerae strain A1552

67.781

93.732

0.635
  pilT Neisseria meningitidis 8013

64.058

98.291

0.63
  pilT Neisseria gonorrhoeae MS11

63.478

98.291

0.624
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

98.291

0.496
  pilU Acinetobacter baylyi ADP1

41.329

98.575

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.003

96.581

0.396
  pilU Vibrio cholerae strain A1552

43.034

92.023

0.396

Multiple sequence alignment