Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AADH33_RS11345 Genome accession   NZ_CP151283
Coordinates   2743622..2744743 (+) Length   373 a.a.
NCBI ID   WP_075101772.1    Uniprot ID   A0AAW4IQV8
Organism   Psychrobacter sp. KFRI-CH2-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2738622..2749743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADH33_RS11330 (AADH33_11320) - 2740086..2741360 (+) 1275 WP_348548939.1 hemolysin family protein -
  AADH33_RS11335 (AADH33_11325) - 2741395..2742090 (-) 696 WP_348548940.1 YggS family pyridoxal phosphate-dependent enzyme -
  AADH33_RS11340 (AADH33_11330) pilT 2742442..2743497 (+) 1056 WP_075101771.1 type IV pilus twitching motility protein PilT Machinery gene
  AADH33_RS11345 (AADH33_11335) pilU 2743622..2744743 (+) 1122 WP_075101772.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AADH33_RS11350 (AADH33_11340) fur 2744899..2745333 (-) 435 WP_075101773.1 ferric iron uptake transcriptional regulator -
  AADH33_RS11355 (AADH33_11345) bamE 2745696..2746115 (+) 420 WP_083608584.1 outer membrane protein assembly factor BamE -
  AADH33_RS11360 (AADH33_11350) - 2746135..2746467 (-) 333 WP_256713751.1 RnfH family protein -
  AADH33_RS11365 (AADH33_11355) - 2746455..2747597 (-) 1143 WP_348548941.1 hypothetical protein -
  AADH33_RS11370 (AADH33_11360) - 2748023..2748691 (-) 669 WP_075101776.1 c-type cytochrome -
  AADH33_RS11375 (AADH33_11365) - 2749009..2749341 (-) 333 WP_207970238.1 c-type cytochrome -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41696.03 Da        Isoelectric Point: 6.9361

>NTDB_id=976081 AADH33_RS11345 WP_075101772.1 2743622..2744743(+) (pilU) [Psychrobacter sp. KFRI-CH2-11]
MDIDKLLQLMINKNGSDLFITAEVAPSMKVNGKILPVGKTPLTPAQTLKLVQSVMTASQQQEFEETNECQFAITDEAKQA
RFRVSAFVQRDMAGMILRKIENKIPTVEELRLPPVLKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSQGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEIHEIKDVMKRSRESGMQTFDQ
ALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNSAHLLDEGADGLSLDIT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=976081 AADH33_RS11345 WP_075101772.1 2743622..2744743(+) (pilU) [Psychrobacter sp. KFRI-CH2-11]
ATGGACATTGATAAACTATTGCAGTTAATGATCAATAAAAACGGCTCTGACCTTTTTATTACTGCTGAAGTGGCACCTTC
GATGAAGGTGAACGGCAAGATACTGCCTGTGGGTAAGACCCCGCTGACCCCTGCCCAAACTCTAAAACTGGTCCAAAGCG
TCATGACGGCCAGTCAGCAGCAAGAGTTTGAAGAGACCAACGAGTGTCAGTTTGCCATCACTGATGAAGCCAAGCAGGCA
CGGTTTCGGGTCAGCGCGTTTGTACAGCGCGACATGGCTGGGATGATTTTGCGTAAAATTGAAAACAAAATCCCAACCGT
CGAAGAGCTGCGCCTGCCACCTGTTCTCAAAGAGCTGGCGATGAAAAAGCGCGGTATCATCCTACTGGTCGGTGCGACAG
GTACGGGTAAATCGACGACGCTTGCTGCCATGATCGGCCATCGCAACCAGAACTCGCAAGGCCATATCATTACCATCGAA
GACCCGATCGAGTTTGTCCATAAGCATCGCGGCTGTATCATCACCCAGCGCGAGGTCGGTATTGACACCCACTCTTTTGA
AGCAGGTTTAAAAAACACCCTGCGCCAAGCCCCTGATGTCATCTTGATTGGTGAGATTCGTAACCGCGAGGTCATGAGCT
ACGCGATTCAGTATGCTGAGACGGGGCATTTGGTATTTGCCACGCTACACGCCAACAACGCCAACCAAGCCATTGATCGT
ATTATTCACTTTTTTGAGACCAGCCGTCACAACCAGCTGTTTATGGATTTGTCGCTCAACCTGCAAGCGATCATCGCCCA
GCAGCTCATCCCAACACCGGATGGCAAAGGGCGACGCGCGGCGATTGAGATTTTATTGAACTCCCAGCTCATCAGCGACT
ATATCCGTAAAGGCGAGATTCATGAGATTAAAGATGTGATGAAGCGCTCACGAGAGAGCGGTATGCAGACCTTCGATCAG
GCACTGTTTGATTTATATGAAAACGGCGAGATTACCTATAAAGAAGCGATCTTGCATGCTGATTCACCAAACGATTTGCG
CCTAAAAATCAAGCTCAGTAGCAAAAACAGCGCCCACCTTTTGGACGAAGGCGCAGACGGTTTGTCGCTAGATATCACAT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.22

100

0.662
  pilU Acinetobacter baylyi ADP1

68.169

95.174

0.649
  pilU Vibrio cholerae strain A1552

53.846

94.102

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.5

96.515

0.41
  pilT Legionella pneumophila strain Lp02

42.462

87.131

0.37
  pilT Legionella pneumophila strain ERS1305867

42.462

87.131

0.37
  pilT Acinetobacter nosocomialis M2

39.942

91.957

0.367
  pilT Acinetobacter baumannii strain A118

39.942

91.957

0.367
  pilT Acinetobacter baumannii D1279779

39.942

91.957

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.597

89.812

0.365
  pilT Vibrio cholerae strain A1552

40.597

89.812

0.365
  pilT Pseudomonas aeruginosa PAK

39.306

92.761

0.365

Multiple sequence alignment