Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OO861_RS19975 Genome accession   NZ_AP026886
Coordinates   4108066..4109046 (-) Length   326 a.a.
NCBI ID   WP_073001283.1    Uniprot ID   A0A7W3C5R8
Organism   Enterobacter asburiae strain KAM576     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4103066..4114046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO861_RS19945 (KAM576c_39070) - 4103942..4104439 (+) 498 WP_045888327.1 SprT family zinc-dependent metalloprotease -
  OO861_RS19950 (KAM576c_39080) endA 4104534..4105241 (+) 708 WP_023333343.1 deoxyribonuclease I -
  OO861_RS19955 (KAM576c_39090) rsmE 4105293..4106024 (+) 732 WP_058841908.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OO861_RS19960 (KAM576c_39100) gshB 4106044..4106991 (+) 948 WP_029739222.1 glutathione synthase -
  OO861_RS19965 (KAM576c_39110) - 4107079..4107639 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  OO861_RS19970 (KAM576c_39120) ruvX 4107639..4108055 (+) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  OO861_RS19975 (KAM576c_39130) pilT 4108066..4109046 (-) 981 WP_073001283.1 type IV pilus twitching motility protein PilT Machinery gene
  OO861_RS19980 (KAM576c_39140) - 4109064..4109765 (+) 702 WP_143346507.1 YggS family pyridoxal phosphate-dependent enzyme -
  OO861_RS19985 (KAM576c_39150) - 4109787..4110353 (+) 567 WP_033146608.1 YggT family protein -
  OO861_RS19990 (KAM576c_39160) yggU 4110350..4110646 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  OO861_RS19995 (KAM576c_39170) - 4110650..4111243 (+) 594 WP_064673281.1 XTP/dITP diphosphatase -
  OO861_RS20000 (KAM576c_39180) hemW 4111236..4112378 (+) 1143 WP_182382664.1 radical SAM family heme chaperone HemW -
  OO861_RS20005 (KAM576c_39190) - 4112442..4112804 (+) 363 WP_071891574.1 endonuclease domain-containing protein -
  OO861_RS20010 (KAM576c_39200) - 4112866..4113582 (-) 717 WP_069303288.1 DUF2884 domain-containing protein -
  OO861_RS20015 (KAM576c_39210) - 4113639..4113965 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35506.56 Da        Isoelectric Point: 6.9108

>NTDB_id=97345 OO861_RS19975 WP_073001283.1 4108066..4109046(-) (pilT) [Enterobacter asburiae strain KAM576]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGIWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSLTLRLLPRSCPQLSSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRSQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=97345 OO861_RS19975 WP_073001283.1 4108066..4109046(-) (pilT) [Enterobacter asburiae strain KAM576]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGTCGGCTTGAACCCGCTCCCTTTCCGCCTCCCGATGTCGGAGCGTTATTAAAAGCATGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGGCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTTTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCACAGCTCTCTTCGCTGGG
CGCGCCGCGGGCTATCCCGGAACTGTTGTCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAATCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATCCAGCAGCGTGAGATAGGCCAGCACAGCCCGTCATTTGCCGAAGCGCTGCG
CAGTGCCTTGCGCCAGGATCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAGCCAGCTGGCCGGTAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATCCAGACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7W3C5R8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Legionella pneumophila strain Lp02

49.689

98.773

0.491
  pilT Legionella pneumophila strain ERS1305867

49.689

98.773

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment