Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AABD45_RS00205 Genome accession   NZ_CP150831
Coordinates   26727..27833 (+) Length   368 a.a.
NCBI ID   WP_038963182.1    Uniprot ID   -
Organism   Vibrio vulnificus strain Per1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 21727..32833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABD45_RS00170 (AABD45_00170) - 22107..22709 (-) 603 WP_038964745.1 XTP/dITP diphosphatase -
  AABD45_RS00175 (AABD45_00175) - 22720..23151 (-) 432 WP_038941482.1 DUF4426 domain-containing protein -
  AABD45_RS00180 (AABD45_00180) yggU 23194..23484 (-) 291 WP_013571094.1 DUF167 family protein YggU -
  AABD45_RS00185 (AABD45_00185) - 23484..24041 (-) 558 WP_013571095.1 YggT family protein -
  AABD45_RS00190 (AABD45_00190) proC 24083..24901 (-) 819 WP_045589551.1 pyrroline-5-carboxylate reductase -
  AABD45_RS00195 (AABD45_00195) - 24936..25646 (-) 711 WP_039449191.1 YggS family pyridoxal phosphate-dependent enzyme -
  AABD45_RS00200 (AABD45_00200) pilT 25671..26708 (+) 1038 WP_026050359.1 type IV pilus twitching motility protein PilT Machinery gene
  AABD45_RS00205 (AABD45_00205) pilU 26727..27833 (+) 1107 WP_038963182.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AABD45_RS00210 (AABD45_00210) ruvX 27924..28346 (-) 423 WP_011079464.1 Holliday junction resolvase RuvX -
  AABD45_RS00215 (AABD45_00215) - 28368..28931 (-) 564 WP_039540484.1 YqgE/AlgH family protein -
  AABD45_RS00220 (AABD45_00220) gshB 28985..29938 (-) 954 WP_039449189.1 glutathione synthase -
  AABD45_RS00225 (AABD45_00225) rsmE 29953..30684 (-) 732 WP_017428735.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AABD45_RS00230 (AABD45_00230) - 30921..32255 (+) 1335 WP_406849123.1 hypothetical protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41223.42 Da        Isoelectric Point: 7.3024

>NTDB_id=972706 AABD45_RS00205 WP_038963182.1 26727..27833(+) (pilU) [Vibrio vulnificus strain Per1]
MDLNKILEGMITLKASDLYITVGAPVLFRVDGELRPQGEKLSQIEVAQLLDGAMDEDRRADFRKSRESNFAIVRDSGRFR
VSAFYQRELPGAVIRRIETNIPTFEELKLPHVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRGGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEVLLNSPRVSDLIRRGDLHELKMTMAKSKEVGMQTFDQSLY
KLVVKGKISEEDALHSADSANDLRLMLKTQRGDLSTPGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=972706 AABD45_RS00205 WP_038963182.1 26727..27833(+) (pilU) [Vibrio vulnificus strain Per1]
ATGGATTTAAACAAGATTTTAGAAGGGATGATCACGCTTAAAGCGTCGGATCTTTATATCACGGTTGGTGCCCCCGTGCT
GTTTCGTGTCGATGGTGAGCTGCGGCCGCAAGGCGAAAAATTATCGCAAATTGAAGTAGCTCAACTGCTGGATGGTGCGA
TGGACGAAGATCGGCGCGCTGACTTTCGCAAAAGCCGCGAGTCAAACTTTGCCATTGTGCGAGATTCGGGCCGTTTTCGT
GTCAGTGCCTTCTACCAACGAGAGCTTCCTGGTGCGGTAATTCGCCGCATTGAAACCAACATTCCAACCTTTGAGGAACT
TAAGCTGCCTCATGTTTTGCAAGACTTAGCGATTGCCAAGCGTGGATTGGTGCTGGTGGTGGGGGCAACGGGCTCGGGTA
AATCGACCACCATGGCTGCGATGACAGGCTATCGAAATACCCACCGTGGTGGACATATTTTGACGGTGGAAGACCCAATT
GAATTTGTTCACGAACATAAACGCTGTATTGTCACCCAGCGAGAAGTCGGCTTGGATACGGAAAGCTATGAAGTCGCATT
GAAGAACTCGCTGCGTCAAGCGCCAGATATGATTCTGATCGGTGAGATTCGCAGTCGCGAAACCATGGAATACGCGATGA
CGTTTGCTGAAACGGGTCACTTGTGTATGGCGACGCTGCATGCCAACAACGCCAACCAAGCGCTGGAACGCATCTTACAC
TTGGTGCCTAAAGAGCAAAAAGAGCAGTTTTTGTTTGACCTTTCCATGAACCTAAAAGGGGTGGTGGGTCAGCAGTTGAT
TCGTGACAAAAATGGTAAAGGTCGTCACGGTGTGTTCGAGGTGTTACTTAACAGCCCACGAGTGTCGGATTTGATCCGTC
GTGGCGATTTGCATGAGCTGAAAATGACCATGGCGAAGTCGAAAGAGGTCGGTATGCAGACCTTTGACCAATCACTCTAT
AAATTAGTGGTGAAAGGAAAAATCAGTGAGGAAGACGCGTTGCACAGTGCTGACTCAGCGAACGACTTACGTTTAATGTT
GAAAACACAACGAGGCGATCTTAGCACTCCCGGTTCTTTGGCAAACGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.511

100

0.845
  pilU Pseudomonas stutzeri DSM 10701

59.429

95.109

0.565
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.658

92.12

0.402
  pilT Acinetobacter baylyi ADP1

42.522

92.663

0.394
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.761

0.361
  pilT Vibrio cholerae strain A1552

39.82

90.761

0.361

Multiple sequence alignment