Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACL60T_RS04370 Genome accession   NZ_CP178659
Coordinates   635450..636883 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain VTB96933v     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 630450..641883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL60T_RS04335 (ACL60T_04335) elbB 631142..631795 (+) 654 WP_001469263.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACL60T_RS04340 (ACL60T_04340) mtgA 631792..632520 (+) 729 WP_000047061.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACL60T_RS04345 (ACL60T_04345) yrbL 632517..633149 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ACL60T_RS04350 (ACL60T_04350) npr 633362..633634 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACL60T_RS04355 (ACL60T_04355) rapZ 633631..634485 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACL60T_RS04360 (ACL60T_04360) ptsN 634531..635022 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACL60T_RS04365 (ACL60T_04365) hpf 635140..635427 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACL60T_RS04370 (ACL60T_04370) rpoN/rpoN1 635450..636883 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ACL60T_RS04375 (ACL60T_04375) lptB 636931..637656 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACL60T_RS04380 (ACL60T_04380) lptA 637663..638220 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACL60T_RS04385 (ACL60T_04385) lptC 638189..638764 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACL60T_RS04390 (ACL60T_04390) kdsC 638761..639327 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACL60T_RS04395 (ACL60T_04395) kdsD 639348..640334 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACL60T_RS04400 (ACL60T_04400) yrbG 640348..641325 (-) 978 WP_000922865.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=972705 ACL60T_RS04370 WP_000809051.1 635450..636883(-) (rpoN/rpoN1) [Escherichia coli strain VTB96933v]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=972705 ACL60T_RS04370 WP_000809051.1 635450..636883(-) (rpoN/rpoN1) [Escherichia coli strain VTB96933v]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CTGACACTGACCGCGCCATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCAGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGG
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465

Multiple sequence alignment