Detailed information    

experimental Experimentally validated

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RS_RS02020 Genome accession   NC_003295
Coordinates   435559..437058 (-) Length   499 a.a.
NCBI ID   WP_011000369.1    Uniprot ID   A0A223GJ62
Organism   Ralstonia pseudosolanacearum GMI1000     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

rpoN1 controls other important functions such twitching motility, natural transformation and growth on nitrate, unlike rpoN2.


Genomic Context

Location: 430559..442058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS_RS01990 (RS03370) - 431067..431717 (-) 651 WP_011000364.1 LON peptidase substrate-binding domain-containing protein -
  RS_RS01995 (RS03371) rapZ 431795..432685 (-) 891 WP_011000365.1 RNase adapter RapZ -
  RS_RS02000 (RS03372) - 432779..433114 (-) 336 WP_043876703.1 PsiF family protein -
  RS_RS02005 (RS03373) hprK 433305..434279 (-) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  RS_RS02010 (RS03374) ptsN 434350..434805 (-) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RS_RS02015 (RS03375) hpf 435154..435507 (-) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RS_RS02020 (RS03376) rpoN/rpoN1 435559..437058 (-) 1500 WP_011000369.1 RNA polymerase factor sigma-54 Machinery gene
  RS_RS02025 (RS03377) lptB 437156..437944 (-) 789 WP_011000370.1 LPS export ABC transporter ATP-binding protein -
  RS_RS02030 (RS03378) lptA 437978..438553 (-) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  RS_RS02035 (RS03379) lptC 438622..439254 (-) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  RS_RS02040 (RS03380) - 439256..439843 (-) 588 WP_011000373.1 KdsC family phosphatase -
  RS_RS02045 (RS03381) - 439882..440865 (-) 984 WP_016724265.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55227.56 Da        Isoelectric Point: 4.8064

>NTDB_id=1425 RS_RS02020 WP_011000369.1 435559..437058(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum GMI1000]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTAEASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1425 RS_RS02020 WP_011000369.1 435559..437058(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum GMI1000]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCATGCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAACGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223GJ62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Suvendra K Ray et al. (2015) rpoN1, but not rpoN2, is required for twitching motility, natural competence, growth on nitrate, and virulence of Ralstonia solanacearum. Frontiers in Microbiology 6:229. [PMID: 25852679]