Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WJM45_RS00640 Genome accession   NZ_CP150634
Coordinates   142397..143434 (+) Length   345 a.a.
NCBI ID   WP_341327079.1    Uniprot ID   -
Organism   Methylotuvimicrobium sp. KM2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 137397..148434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM45_RS00615 (WJM45_00615) - 138668..140059 (-) 1392 WP_341327074.1 selenium-binding protein SBP56-related protein -
  WJM45_RS00620 (WJM45_00620) - 140159..140434 (-) 276 WP_341327075.1 DUF4242 domain-containing protein -
  WJM45_RS00625 (WJM45_00625) - 140486..141082 (-) 597 WP_341327076.1 TetR family transcriptional regulator C-terminal domain-containing protein -
  WJM45_RS00630 (WJM45_00630) - 141375..141581 (+) 207 WP_341327077.1 hypothetical protein -
  WJM45_RS00635 (WJM45_00635) - 141600..142283 (-) 684 WP_341327078.1 YggS family pyridoxal phosphate-dependent enzyme -
  WJM45_RS00640 (WJM45_00640) pilT 142397..143434 (+) 1038 WP_341327079.1 type IV pilus twitching motility protein PilT Machinery gene
  WJM45_RS00645 (WJM45_00645) pilU 143447..144580 (+) 1134 WP_341327080.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WJM45_RS00650 (WJM45_00650) djlA 144586..145395 (-) 810 WP_341327081.1 co-chaperone DjlA -
  WJM45_RS00655 (WJM45_00655) - 145470..146369 (+) 900 WP_341327082.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  WJM45_RS00660 (WJM45_00660) radC 146629..147303 (-) 675 WP_341327083.1 DNA repair protein RadC -
  WJM45_RS00665 (WJM45_00665) - 147437..147631 (-) 195 WP_341327084.1 hypothetical protein -
  WJM45_RS00670 (WJM45_00670) - 147630..147755 (+) 126 WP_341327085.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38327.18 Da        Isoelectric Point: 6.8402

>NTDB_id=970735 WJM45_RS00640 WP_341327079.1 142397..143434(+) (pilT) [Methylotuvimicrobium sp. KM2]
MDIAELLTFSVKNNASDLHLSAGLPPMIRVDGDIRRINIPALEHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLSLEDLNAPKFFEEVTRKPRGLILVTGPTGSGKSTTLAAMINHINTNDYAHILTVED
PIEFVHESQKCLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTAAIRNLIREAKVAQMYSAIQTGRKEGMQTLDQNL
KELVEKGLVASKVAMLKAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=970735 WJM45_RS00640 WP_341327079.1 142397..143434(+) (pilT) [Methylotuvimicrobium sp. KM2]
ATGGATATTGCTGAATTATTGACTTTTTCCGTTAAAAACAATGCATCGGATTTGCATTTATCGGCTGGTTTGCCGCCGAT
GATTCGAGTGGACGGCGATATCCGGCGTATCAATATTCCTGCCTTAGAGCATAAAGAAGTTCATGCCTTGATTTACGACA
TCATGAACGATAAACAGCGCCGCGATTATGAAGAATTTCTTGAAACTGATTTTTCTTTTGCATTGCCGGGTGTTGCAAGG
TTTCGTGTCAATGCATTCAATCAGGATCGAGGTGCGGGGGCGGTTTTTAGAACGATTCCTTCGAAGGTGTTGAGTTTAGA
GGATCTAAATGCGCCAAAGTTTTTTGAAGAAGTTACGCGCAAACCGAGAGGTTTAATACTGGTAACAGGACCTACCGGAT
CGGGTAAATCGACGACGCTCGCGGCGATGATTAACCATATCAACACCAATGATTATGCACATATTTTAACCGTTGAGGAC
CCGATCGAGTTCGTCCATGAAAGCCAAAAATGCTTGATCAACCAACGAGAAGTCCATCGTGATACATTAGGTTTCAACGA
GGCCTTGCGATCCGCGTTGCGGGAAGACCCCGACGTTATTTTAGTGGGCGAAATGCGCGACTTGGAAACGATAAGATTGG
CACTGACTGCGGCAGAAACCGGTCACTTGGTTTTCGGCACGTTGCACACCACGTCGGCCGCGAAAACGATCGACCGCATT
ATCGATGTGTTTCCGGCGGCTGAAAAAGACATGATCCGCTCGATGTTGTCGGAATCGTTGCAAGCGGTTATTTCGCAAAC
CTTACTGAAAAAAGTCGGTGGAGGACGTATCGCGGCGCATGAAATCATGGTTGGGACTGCAGCCATTAGAAACTTGATTC
GCGAAGCAAAAGTCGCGCAAATGTATTCGGCGATTCAAACCGGGCGCAAGGAAGGCATGCAGACCTTGGATCAAAATTTG
AAAGAATTGGTTGAAAAAGGCTTAGTGGCTTCAAAAGTCGCCATGTTGAAAGCGGTTAACAAAGATATGTTCAGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

80.178

97.971

0.786
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Legionella pneumophila strain Lp02

73.256

99.71

0.73
  pilT Legionella pneumophila strain ERS1305867

73.256

99.71

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.783

96.232

0.681
  pilT Vibrio cholerae strain A1552

70.783

96.232

0.681
  pilT Neisseria meningitidis 8013

67.055

99.42

0.667
  pilT Neisseria gonorrhoeae MS11

66.472

99.42

0.661
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.504
  pilU Acinetobacter baylyi ADP1

40.058

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

39.595

100

0.397
  pilU Vibrio cholerae strain A1552

40

94.203

0.377

Multiple sequence alignment