Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WJM45_RS00645 Genome accession   NZ_CP150634
Coordinates   143447..144580 (+) Length   377 a.a.
NCBI ID   WP_341327080.1    Uniprot ID   -
Organism   Methylotuvimicrobium sp. KM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 138447..149580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM45_RS00615 (WJM45_00615) - 138668..140059 (-) 1392 WP_341327074.1 selenium-binding protein SBP56-related protein -
  WJM45_RS00620 (WJM45_00620) - 140159..140434 (-) 276 WP_341327075.1 DUF4242 domain-containing protein -
  WJM45_RS00625 (WJM45_00625) - 140486..141082 (-) 597 WP_341327076.1 TetR family transcriptional regulator C-terminal domain-containing protein -
  WJM45_RS00630 (WJM45_00630) - 141375..141581 (+) 207 WP_341327077.1 hypothetical protein -
  WJM45_RS00635 (WJM45_00635) - 141600..142283 (-) 684 WP_341327078.1 YggS family pyridoxal phosphate-dependent enzyme -
  WJM45_RS00640 (WJM45_00640) pilT 142397..143434 (+) 1038 WP_341327079.1 type IV pilus twitching motility protein PilT Machinery gene
  WJM45_RS00645 (WJM45_00645) pilU 143447..144580 (+) 1134 WP_341327080.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WJM45_RS00650 (WJM45_00650) djlA 144586..145395 (-) 810 WP_341327081.1 co-chaperone DjlA -
  WJM45_RS00655 (WJM45_00655) - 145470..146369 (+) 900 WP_341327082.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  WJM45_RS00660 (WJM45_00660) radC 146629..147303 (-) 675 WP_341327083.1 DNA repair protein RadC -
  WJM45_RS00665 (WJM45_00665) - 147437..147631 (-) 195 WP_341327084.1 hypothetical protein -
  WJM45_RS00670 (WJM45_00670) - 147630..147755 (+) 126 WP_341327085.1 hypothetical protein -
  WJM45_RS00675 (WJM45_00675) coaBC 147742..148959 (+) 1218 WP_341327086.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  WJM45_RS00680 (WJM45_00680) dut 148928..149383 (+) 456 WP_341327087.1 dUTP diphosphatase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42518.79 Da        Isoelectric Point: 6.4444

>NTDB_id=970736 WJM45_RS00645 WP_341327080.1 143447..144580(+) (pilU) [Methylotuvimicrobium sp. KM2]
MDEFKRLLELMVQQKASDLFITAGRPPTIKVDGKLIEVSKTTLNNDQSRAIVMSVMTQRQKDEFDNTKECQFAIAFPKLG
RFRVSAFTQRDAAGMVLRRIETHIPDAEDLHLPPVLKDLVMNKRGLVLFVGGTGTGKSTSLAALIKYRNQNSSGHIITIE
DPLEFLHPHLGCIVTQREVGMDTESYEVALKNTLRQAPDVILIGEVRTRETMQNAITFAETGHLCITTLHANNANQALDR
ILHFFPDEMHNQLFMDLSLNLRGIVAQQLIARADGKGRYPAVEILLNTPLVSDLIRKGEVHKLKELMKNSREHGMQTFDQ
ALYDLYTAGKISYEDALNSADSRNEVRLMIKLGAEDLGTLVNENMHLKEDEDEHVRF

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=970736 WJM45_RS00645 WP_341327080.1 143447..144580(+) (pilU) [Methylotuvimicrobium sp. KM2]
ATGGATGAATTTAAGCGACTACTTGAGTTAATGGTTCAGCAAAAGGCCTCCGATTTATTCATCACTGCGGGGCGTCCGCC
CACGATAAAAGTCGACGGTAAGTTGATCGAAGTATCCAAAACGACGCTAAACAACGATCAATCTCGAGCCATTGTCATGA
GTGTGATGACACAACGGCAAAAAGACGAATTCGATAATACCAAAGAGTGTCAGTTTGCGATTGCCTTTCCTAAGCTTGGC
CGATTTCGGGTGAGTGCGTTTACCCAGAGAGATGCTGCGGGAATGGTGTTGAGACGCATCGAGACCCATATTCCCGATGC
CGAAGACCTGCATTTGCCGCCGGTTTTAAAAGATTTGGTCATGAACAAGCGCGGACTGGTGTTGTTCGTCGGCGGGACGG
GAACCGGTAAGTCGACTTCATTGGCGGCATTGATAAAATACCGAAACCAAAACAGCAGCGGTCATATAATTACCATTGAA
GACCCGCTCGAGTTTTTGCATCCGCATTTGGGTTGCATCGTTACGCAGCGCGAAGTCGGCATGGATACCGAATCCTATGA
AGTCGCGCTTAAGAATACCTTGCGCCAAGCGCCTGATGTTATCCTGATCGGTGAGGTTAGAACCCGTGAAACGATGCAAA
ATGCGATTACGTTCGCCGAAACCGGGCATTTGTGTATAACGACCTTGCATGCGAACAATGCCAATCAGGCATTGGATCGG
ATTTTGCATTTTTTCCCGGACGAAATGCATAATCAATTATTTATGGATTTGTCGCTGAATTTGCGAGGCATTGTCGCGCA
GCAGTTGATTGCCCGTGCCGATGGCAAAGGACGCTATCCCGCAGTTGAAATTTTGTTGAACACACCCTTGGTTTCCGATT
TGATTCGTAAAGGCGAAGTTCACAAACTTAAGGAATTAATGAAGAATTCGCGGGAACATGGCATGCAGACCTTCGATCAA
GCGCTTTACGATCTCTATACCGCCGGAAAAATTAGTTATGAAGATGCCTTAAACTCTGCGGACTCAAGAAACGAAGTGCG
CTTGATGATTAAACTGGGTGCCGAAGACTTAGGGACTTTAGTCAATGAAAATATGCATCTAAAAGAAGACGAAGACGAGC
ATGTTCGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.782

100

0.658
  pilU Acinetobacter baylyi ADP1

62.994

93.899

0.592
  pilU Vibrio cholerae strain A1552

58.453

92.573

0.541
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.566

90.981

0.387
  pilT Legionella pneumophila strain Lp02

42.308

89.655

0.379
  pilT Legionella pneumophila strain ERS1305867

42.308

89.655

0.379
  pilT Pseudomonas aeruginosa PAK

40.719

88.594

0.361

Multiple sequence alignment