Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WIF35_RS13485 Genome accession   NZ_CP150631
Coordinates   2772048..2773091 (+) Length   347 a.a.
NCBI ID   WP_412478735.1    Uniprot ID   -
Organism   Azonexus sp. IMCC34839     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2767048..2778091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIF35_RS13455 - 2767465..2768424 (+) 960 WP_412478729.1 aspartate carbamoyltransferase catalytic subunit -
  WIF35_RS13460 - 2768429..2769679 (+) 1251 WP_412478730.1 dihydroorotase -
  WIF35_RS13465 - 2769667..2769963 (-) 297 WP_412478731.1 DUF167 domain-containing protein -
  WIF35_RS13470 - 2769960..2770493 (-) 534 WP_412478732.1 YggT family protein -
  WIF35_RS13475 proC 2770493..2771305 (-) 813 WP_412478733.1 pyrroline-5-carboxylate reductase -
  WIF35_RS13480 - 2771302..2771991 (-) 690 WP_412478734.1 YggS family pyridoxal phosphate-dependent enzyme -
  WIF35_RS13485 pilT 2772048..2773091 (+) 1044 WP_412478735.1 type IV pilus twitching motility protein PilT Machinery gene
  WIF35_RS13490 pilU 2773125..2774261 (+) 1137 WP_412478736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WIF35_RS13495 - 2774273..2774959 (+) 687 WP_412478737.1 DUF4197 domain-containing protein -
  WIF35_RS13500 - 2775008..2775328 (+) 321 WP_412478738.1 hypothetical protein -
  WIF35_RS13505 - 2775445..2777436 (+) 1992 WP_412478739.1 UvrD-helicase domain-containing protein -
  WIF35_RS13510 - 2777518..2777961 (-) 444 WP_412478740.1 c-type cytochrome -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38487.23 Da        Isoelectric Point: 6.8809

>NTDB_id=970731 WIF35_RS13485 WP_412478735.1 2772048..2773091(+) (pilT) [Azonexus sp. IMCC34839]
MDITELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHSMVYDIMNDGQRKVYEETLECDFSFEIPNLAR
FRVNAFNQQRGAGAVFRTIPSKVLTLEELNCPKIFKEISEFPRGIVLVTGPTGSGKSTTLAAMINHINENDYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQNFGMQTLDQC
LIDLVRRNVVSSNEAKLRAANKDAFPA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=970731 WIF35_RS13485 WP_412478735.1 2772048..2773091(+) (pilT) [Azonexus sp. IMCC34839]
ATGGATATCACCGAACTGCTGGCCTTCAGCGTCAAGAACAAGGCTTCCGACCTTCACCTTTCGGCCGGTTTGCCGCCGAT
GATCCGCGTGCACGGTGACGTGCGCCGCATCAACCTGCCGCCGATGGAGCACAAGGACGTGCACAGCATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGGTGTATGAAGAGACGCTGGAGTGCGACTTTTCCTTCGAGATTCCCAACCTGGCCCGT
TTCCGGGTCAATGCTTTCAACCAGCAGCGTGGCGCGGGGGCCGTTTTCCGGACCATTCCGTCCAAGGTGTTGACGCTGGA
AGAGTTGAACTGCCCGAAGATCTTCAAGGAAATTTCCGAGTTTCCGCGCGGCATCGTGCTGGTCACCGGGCCGACCGGTT
CCGGTAAATCGACCACGCTGGCGGCGATGATCAACCACATCAACGAAAACGATTATGGTCACATCCTGACGGTCGAGGAC
CCGATCGAATTCGTCCATGAGTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGTCCGCATACGCTGTCCTTCTCCAA
TGCGCTACGCTCGGCACTGCGTGAGGATCCGGACGTGATCCTGGTCGGTGAAATGCGCGACCTGGAAACTATCCGCCTGG
CCCTGACCGCGGCCGAAACCGGCCACTTGGTGTTCGGTACGCTGCATACCTCGTCGGCCGCCAAGACCATCGACCGTGTG
GTCGACGTCTTTCCGGCGGCGGAAAAGGAAATGGTGCGTTCGATGTTGTCCGAATCCCTGCGCGCGGTCATTTCGCAAAC
CCTGCTCAAGACCAAGGACGGTTCCGGCCGGGTCGCGGCGCACGAGATCATGATCGGCACGCCGGCCATCCGTAACCTGA
TCCGTGAAAACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCCAGAACTTCGGCATGCAGACACTCGATCAGTGC
CTGATCGATCTGGTCCGCCGCAATGTCGTGTCCAGCAACGAAGCCAAGCTGCGTGCCGCCAACAAGGACGCTTTCCCGGC
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.71

99.424

0.793
  pilT Acinetobacter baumannii D1279779

79.71

99.424

0.793
  pilT Acinetobacter baumannii strain A118

79.71

99.424

0.793
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

75.942

99.424

0.755
  pilT Legionella pneumophila strain Lp02

73.913

99.424

0.735
  pilT Legionella pneumophila strain ERS1305867

73.913

99.424

0.735
  pilT Neisseria meningitidis 8013

72.464

99.424

0.72
  pilT Neisseria gonorrhoeae MS11

72.174

99.424

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

96.83

0.669
  pilT Vibrio cholerae strain A1552

69.048

96.83

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.424

0.519
  pilU Pseudomonas stutzeri DSM 10701

41.593

97.695

0.406
  pilU Acinetobacter baylyi ADP1

41.667

96.83

0.403
  pilU Vibrio cholerae strain A1552

40.896

96.542

0.395

Multiple sequence alignment