Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WIF35_RS13490 Genome accession   NZ_CP150631
Coordinates   2773125..2774261 (+) Length   378 a.a.
NCBI ID   WP_412478736.1    Uniprot ID   -
Organism   Azonexus sp. IMCC34839     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2768125..2779261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIF35_RS13460 - 2768429..2769679 (+) 1251 WP_412478730.1 dihydroorotase -
  WIF35_RS13465 - 2769667..2769963 (-) 297 WP_412478731.1 DUF167 domain-containing protein -
  WIF35_RS13470 - 2769960..2770493 (-) 534 WP_412478732.1 YggT family protein -
  WIF35_RS13475 proC 2770493..2771305 (-) 813 WP_412478733.1 pyrroline-5-carboxylate reductase -
  WIF35_RS13480 - 2771302..2771991 (-) 690 WP_412478734.1 YggS family pyridoxal phosphate-dependent enzyme -
  WIF35_RS13485 pilT 2772048..2773091 (+) 1044 WP_412478735.1 type IV pilus twitching motility protein PilT Machinery gene
  WIF35_RS13490 pilU 2773125..2774261 (+) 1137 WP_412478736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WIF35_RS13495 - 2774273..2774959 (+) 687 WP_412478737.1 DUF4197 domain-containing protein -
  WIF35_RS13500 - 2775008..2775328 (+) 321 WP_412478738.1 hypothetical protein -
  WIF35_RS13505 - 2775445..2777436 (+) 1992 WP_412478739.1 UvrD-helicase domain-containing protein -
  WIF35_RS13510 - 2777518..2777961 (-) 444 WP_412478740.1 c-type cytochrome -
  WIF35_RS13515 - 2778278..2778460 (-) 183 WP_412478741.1 hypothetical protein -
  WIF35_RS13520 pncC 2778620..2779123 (+) 504 WP_412478742.1 nicotinamide-nucleotide amidase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42121.52 Da        Isoelectric Point: 6.5059

>NTDB_id=970732 WIF35_RS13490 WP_412478736.1 2773125..2774261(+) (pilU) [Azonexus sp. IMCC34839]
MERDQAMKFMHDLLRLMAQKKGSDLFITAGFPPAIKIDGKITPVSNQVLTAQHTAELARAIMNDRQAAEFEATKECNFAI
SPSGIGRFRVNALVQQGRVGVVCRTINMTIPTIDELQLPPVIKDLAMTKRGLIIFVGGTGTGKTTSLAALVDYRNENSYG
HIITIEDPIEYVHDHKNCIVTQREVGVDTDDWGPALKNTLRQAPDVILMGEIRDRDTMDYAIAFAETGHLALATLHANSS
NQAIDRIINFFPEERRSQLLMDLSLNLRAMVSQRLIPKKDGKGRVAAIEVMLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEAGKITYEDALRNADSLNDLRLQIKLHGKESKDKDLTTGIGHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=970732 WIF35_RS13490 WP_412478736.1 2773125..2774261(+) (pilU) [Azonexus sp. IMCC34839]
ATGGAACGCGATCAGGCAATGAAATTCATGCACGATCTTCTGCGACTGATGGCGCAGAAGAAGGGCTCCGACCTCTTCAT
CACGGCGGGCTTTCCGCCGGCGATCAAGATCGACGGAAAGATCACGCCGGTGTCGAATCAGGTGCTGACGGCACAACACA
CCGCCGAACTGGCGCGTGCCATCATGAACGATCGCCAAGCCGCGGAGTTCGAAGCGACCAAGGAATGCAACTTCGCGATT
TCTCCGTCGGGCATCGGGCGTTTCCGCGTCAATGCGCTGGTCCAGCAGGGGCGCGTCGGCGTCGTCTGCCGGACGATCAA
CATGACCATCCCGACTATCGACGAACTGCAGCTGCCGCCGGTGATCAAGGACCTGGCGATGACCAAGCGCGGCCTGATCA
TCTTCGTCGGTGGCACCGGTACCGGTAAGACGACTTCGCTGGCGGCGCTGGTCGATTACCGCAACGAGAATTCCTACGGT
CACATCATCACGATCGAAGACCCGATCGAATACGTGCACGACCACAAGAACTGCATCGTCACGCAGCGCGAGGTCGGGGT
CGATACGGATGATTGGGGCCCGGCGCTGAAGAACACGCTGCGTCAGGCGCCGGACGTCATCCTGATGGGTGAAATCCGCG
ACCGCGACACGATGGATTACGCCATTGCCTTTGCCGAAACCGGCCACTTGGCGCTGGCCACGCTGCACGCCAATAGCTCG
AACCAGGCAATTGACCGCATCATCAACTTCTTCCCGGAAGAGCGTCGTTCGCAACTGCTGATGGACCTGTCGCTCAACCT
GCGTGCCATGGTGTCGCAGCGCCTGATTCCGAAAAAGGATGGCAAGGGCCGCGTCGCCGCCATCGAGGTGATGCTCAATT
CGCCGCTGATCTCTGATCTCATCTTTAAGGGCGAGGTGCACGAGATCAAGGAAATCATGAAGAAGTCGCGCGAGTTGGGT
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTCTACGAGGCCGGCAAGATTACCTACGAAGATGCCCTGCGCAATGCCGA
TTCGCTCAACGACCTGCGCCTGCAGATCAAGCTGCACGGCAAGGAGTCGAAGGACAAGGATCTGACCACCGGCATCGGTC
ATCTCGATATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.011

96.296

0.616
  pilU Acinetobacter baylyi ADP1

59.61

94.974

0.566
  pilU Vibrio cholerae strain A1552

54.545

90.212

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.179

88.624

0.392
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Legionella pneumophila strain Lp02

42.296

87.566

0.37
  pilT Legionella pneumophila strain ERS1305867

42.296

87.566

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.624

0.368
  pilT Acinetobacter baylyi ADP1

41.246

89.153

0.368
  pilT Acinetobacter nosocomialis M2

41.39

87.566

0.362
  pilT Acinetobacter baumannii D1279779

41.39

87.566

0.362
  pilT Acinetobacter baumannii strain A118

41.39

87.566

0.362

Multiple sequence alignment