Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1467_RS04525 Genome accession   NZ_CP150481
Coordinates   1049187..1050224 (+) Length   345 a.a.
NCBI ID   WP_416307610.1    Uniprot ID   -
Organism   Neptunicella sp. SCSIO 80796     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1044187..1055224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1467_RS04495 - 1045014..1045535 (+) 522 WP_416307604.1 O-acetyl-ADP-ribose deacetylase -
  V1467_RS04500 yaaA 1045625..1046401 (+) 777 WP_416307605.1 peroxide stress protein YaaA -
  V1467_RS04505 - 1046445..1046870 (-) 426 WP_416307606.1 DUF4426 domain-containing protein -
  V1467_RS04510 - 1046930..1047475 (-) 546 WP_416307607.1 YggT family protein -
  V1467_RS04515 proC 1047499..1048317 (-) 819 WP_416307608.1 pyrroline-5-carboxylate reductase -
  V1467_RS04520 - 1048325..1049011 (-) 687 WP_416307609.1 YggS family pyridoxal phosphate-dependent enzyme -
  V1467_RS04525 pilT 1049187..1050224 (+) 1038 WP_416307610.1 type IV pilus twitching motility protein PilT Machinery gene
  V1467_RS04530 pilU 1050236..1051348 (+) 1113 WP_416307611.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V1467_RS04535 - 1051362..1051673 (+) 312 WP_416307612.1 DUF2007 domain-containing protein -
  V1467_RS04540 - 1051744..1052019 (+) 276 WP_416307613.1 hypothetical protein -
  V1467_RS04545 - 1052016..1052879 (-) 864 WP_416307614.1 peptidylprolyl isomerase -
  V1467_RS04550 - 1052952..1054022 (+) 1071 WP_416307615.1 energy transducer TonB -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38295.88 Da        Isoelectric Point: 6.5678

>NTDB_id=970527 V1467_RS04525 WP_416307610.1 1049187..1050224(+) (pilT) [Neptunicella sp. SCSIO 80796]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRRLNIDPLDHKQVHALVYEIMNDQQRKEYEENLETDFSFEVKDLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPQIFKDIINQPTGIVLVTGATGSGKSTTLAAMVDHINAHKREHILTIED
PIEFVHENKLSLINQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVGSGQISAQDAAAKSIDKQPKF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=970527 V1467_RS04525 WP_416307610.1 1049187..1050224(+) (pilT) [Neptunicella sp. SCSIO 80796]
ATGGATATTACCGAACTTTTGGCCTTTAGTGTCAAAAATAACGCCTCGGATTTGCACCTTTCGGCAGGACTACCGCCCAT
TATCAGGGTTGATGGTGAAATGCGCCGGTTAAATATTGATCCGCTGGATCATAAACAGGTGCATGCGCTGGTGTACGAAA
TTATGAATGACCAGCAGCGCAAGGAATATGAAGAAAATCTGGAAACCGACTTTTCCTTTGAGGTAAAAGACTTATCGCGA
TTCAGGGTGAATGCGTTTGTACAAAATCGTGGCGCTGCGGCGGTATTACGTACTATTCCCAGTAAAGTGCTGACACTGGA
TGATTTAGGCGCACCGCAAATTTTTAAAGATATTATCAATCAGCCCACCGGAATCGTACTGGTGACTGGGGCGACCGGTT
CAGGTAAAAGTACCACGCTGGCGGCCATGGTGGATCATATTAATGCGCATAAGCGTGAGCATATTCTAACCATCGAAGAT
CCCATCGAGTTCGTGCACGAAAATAAACTTAGCCTGATTAACCAACGGGAAGTGCATCGTGATACCCACAGTTTTAACAA
CGCACTGCGCAGCGCATTAAGGGAAGATCCGGATGTGATCCTGGTAGGGGAATTACGTGATCTGGAAACGATACGCTTGG
CTATCTCGGCGGCAGAAACCGGGCACCTGGTATTTGGTACCTTGCATACCAACTCTGCACCCAAAACCATTGACCGGATC
ATTGATGTTTTCCCGGCCGAAGAAAAATCTATGGTGCGTTCGATGTTATCCGAATCCTTGCGCGCGGTTATTTCACAAAC
CCTGCTGAAAAAAATCGGCGGCGGGCGAGTGGCGGCACATGAAATTATGATTGGTATTCCGGCAATTCGTAACCTGATCC
GTGAGGACAAAGTGCCGCAAATGTATTCGGTGATTCAGACCGGTCAGGCGCACGGTATGCAAACTATGGATCAGTGCTTG
CAGCGCCTGGTGGGCTCAGGCCAGATTAGTGCCCAGGATGCGGCCGCCAAATCCATTGATAAACAACCCAAATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.035

99.71

0.768
  pilT Acinetobacter baumannii strain A118

77.035

99.71

0.768
  pilT Acinetobacter nosocomialis M2

77.035

99.71

0.768
  pilT Pseudomonas stutzeri DSM 10701

74.709

99.71

0.745
  pilT Acinetobacter baylyi ADP1

74.419

99.71

0.742
  pilT Pseudomonas aeruginosa PAK

75.148

97.971

0.736
  pilT Legionella pneumophila strain Lp02

71.261

98.841

0.704
  pilT Legionella pneumophila strain ERS1305867

71.261

98.841

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

97.681

0.696
  pilT Vibrio cholerae strain A1552

71.217

97.681

0.696
  pilT Neisseria meningitidis 8013

67.164

97.101

0.652
  pilT Neisseria gonorrhoeae MS11

66.866

97.101

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.061

99.42

0.528
  pilU Vibrio cholerae strain A1552

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

41.964

97.391

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.101

0.406

Multiple sequence alignment