Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V1467_RS04530 Genome accession   NZ_CP150481
Coordinates   1050236..1051348 (+) Length   370 a.a.
NCBI ID   WP_416307611.1    Uniprot ID   -
Organism   Neptunicella sp. SCSIO 80796     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1045236..1056348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1467_RS04500 yaaA 1045625..1046401 (+) 777 WP_416307605.1 peroxide stress protein YaaA -
  V1467_RS04505 - 1046445..1046870 (-) 426 WP_416307606.1 DUF4426 domain-containing protein -
  V1467_RS04510 - 1046930..1047475 (-) 546 WP_416307607.1 YggT family protein -
  V1467_RS04515 proC 1047499..1048317 (-) 819 WP_416307608.1 pyrroline-5-carboxylate reductase -
  V1467_RS04520 - 1048325..1049011 (-) 687 WP_416307609.1 YggS family pyridoxal phosphate-dependent enzyme -
  V1467_RS04525 pilT 1049187..1050224 (+) 1038 WP_416307610.1 type IV pilus twitching motility protein PilT Machinery gene
  V1467_RS04530 pilU 1050236..1051348 (+) 1113 WP_416307611.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V1467_RS04535 - 1051362..1051673 (+) 312 WP_416307612.1 DUF2007 domain-containing protein -
  V1467_RS04540 - 1051744..1052019 (+) 276 WP_416307613.1 hypothetical protein -
  V1467_RS04545 - 1052016..1052879 (-) 864 WP_416307614.1 peptidylprolyl isomerase -
  V1467_RS04550 - 1052952..1054022 (+) 1071 WP_416307615.1 energy transducer TonB -
  V1467_RS04555 - 1054034..1055761 (-) 1728 WP_416307616.1 diguanylate cyclase -
  V1467_RS04560 - 1055787..1055963 (-) 177 WP_416307617.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40811.77 Da        Isoelectric Point: 5.2540

>NTDB_id=970528 V1467_RS04530 WP_416307611.1 1050236..1051348(+) (pilU) [Neptunicella sp. SCSIO 80796]
MDLSPFLQQMAETNASDLFVTVGFPVSAKINGQMTPITQQNLTVDQAQALCDSAMNDKQQKEFADTKECNFAIAREGIGR
FRVSAFWQRDLPGMVIRRIVTQIPKADDLGLPDILKDIIMAKRGLVLFVGATGTGKSTSLAALMGHRNQNSRGHILTIED
PIEFVHEHANCVVTQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMEIMEYAMSFADTGHLCVATLHANNANQAIERI
MHLAPKDQHDKLRFDLSLNMRAIVAQQLVPTKDGKGRVAAIEVLLNSPFVRDLIQRNEIGGLKEAMQKGKEQGMQTFDMA
LYDLYKRDLVSLDDALHCADSPNDLRLMIKLDTGEGTSMGSLSGVSIDID

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=970528 V1467_RS04530 WP_416307611.1 1050236..1051348(+) (pilU) [Neptunicella sp. SCSIO 80796]
ATGGATTTATCCCCTTTCTTGCAACAAATGGCTGAAACCAATGCGTCGGATCTGTTTGTTACTGTAGGCTTTCCGGTTAG
CGCCAAAATTAATGGCCAGATGACGCCGATCACCCAGCAAAACTTGACTGTTGATCAGGCGCAGGCGTTATGTGACAGCG
CGATGAATGACAAGCAGCAAAAAGAATTTGCCGACACCAAAGAATGTAATTTCGCCATTGCGCGAGAGGGAATAGGGCGT
TTTCGGGTAAGTGCCTTTTGGCAACGGGATTTACCCGGTATGGTGATTCGCCGCATTGTGACCCAAATTCCCAAGGCTGA
TGACTTGGGGCTACCGGATATTCTTAAAGATATCATTATGGCTAAACGCGGTCTGGTGTTATTTGTGGGGGCCACCGGCA
CCGGTAAATCCACCTCTCTGGCTGCATTGATGGGCCATCGTAACCAGAATTCTCGCGGCCATATTCTGACCATTGAAGAT
CCGATCGAATTTGTCCATGAACATGCTAACTGTGTGGTGACTCAGCGTGAAGTAGGGATTGATACCCAGTCGTTCGACGA
TGCACTGAAAAGCTCGTTGCGCCAGGCGCCCGATGTGATCCTGATTGGGGAAATTCGTTCGATGGAAATCATGGAATACG
CCATGTCTTTTGCCGATACCGGTCATTTATGTGTGGCCACTCTGCACGCCAACAATGCCAATCAGGCCATTGAGCGAATT
ATGCATCTGGCACCCAAAGATCAACACGACAAGCTGCGTTTTGATTTAAGCCTGAATATGCGCGCCATCGTCGCTCAGCA
ATTGGTGCCTACCAAGGACGGCAAAGGCCGGGTGGCCGCCATCGAAGTATTACTCAACTCGCCATTTGTAAGGGATTTGA
TTCAGCGTAATGAAATTGGCGGTTTAAAAGAAGCCATGCAAAAAGGTAAAGAGCAGGGCATGCAAACCTTTGATATGGCC
TTATATGATCTGTACAAACGGGACTTGGTCAGCCTGGATGATGCGCTGCACTGCGCTGATTCACCCAATGATTTGCGCTT
GATGATTAAACTGGATACCGGAGAAGGCACCAGTATGGGTTCGCTGAGCGGCGTTTCTATCGATATAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

57.838

100

0.578
  pilU Pseudomonas stutzeri DSM 10701

58.47

98.919

0.578
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.609

93.243

0.397
  pilT Pseudomonas stutzeri DSM 10701

40

93.243

0.373
  pilT Pseudomonas aeruginosa PAK

40

93.243

0.373
  pilT Legionella pneumophila strain Lp02

39.823

91.622

0.365
  pilT Legionella pneumophila strain ERS1305867

39.823

91.622

0.365
  pilT Acinetobacter nosocomialis M2

39.13

93.243

0.365
  pilT Acinetobacter baumannii D1279779

39.13

93.243

0.365
  pilT Acinetobacter baumannii strain A118

39.13

93.243

0.365

Multiple sequence alignment