Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VUK15_RS07720 Genome accession   NZ_CP150462
Coordinates   447701..448735 (+) Length   344 a.a.
NCBI ID   WP_005297689.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. piscicida strain NH-LM2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442701..453735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUK15_RS07685 (VUK15_07685) - 442944..443348 (+) 405 WP_050787742.1 DUF2884 family protein -
  VUK15_RS07690 (VUK15_07690) hemW 443423..444583 (-) 1161 WP_340642197.1 radical SAM family heme chaperone HemW -
  VUK15_RS07695 (VUK15_07695) - 444583..445176 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  VUK15_RS07700 (VUK15_07700) yggU 445278..445577 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  VUK15_RS07705 (VUK15_07705) - 445574..446131 (-) 558 WP_005297682.1 YggT family protein -
  VUK15_RS07710 (VUK15_07710) proC 446143..446961 (-) 819 WP_005297684.1 pyrroline-5-carboxylate reductase -
  VUK15_RS07715 (VUK15_07715) - 446977..447678 (-) 702 WP_106142333.1 YggS family pyridoxal phosphate-dependent enzyme -
  VUK15_RS07720 (VUK15_07720) pilT 447701..448735 (+) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  VUK15_RS07725 (VUK15_07725) pilU 448747..449853 (+) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VUK15_RS07730 (VUK15_07730) ruvX 450181..450606 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  VUK15_RS07735 (VUK15_07735) - 450603..451166 (-) 564 WP_106142332.1 YqgE/AlgH family protein -
  VUK15_RS07740 (VUK15_07740) gshB 451320..452267 (-) 948 WP_005297697.1 glutathione synthase -
  VUK15_RS07745 (VUK15_07745) rsmE 452288..453019 (-) 732 WP_005297699.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38180.73 Da        Isoelectric Point: 6.1433

>NTDB_id=970370 VUK15_RS07720 WP_005297689.1 447701..448735(+) (pilT) [Photobacterium damselae subsp. piscicida strain NH-LM2]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALEHSEVHRLIFDIMNDAQRRQFEEELEVDFSFELPDVGR
FRVNAFHQSRGCSAVFRTIPMTIPTLESLNVPDVFYKIAQCQRGLVLVTGATGSGKSTTIAALVDYINEHTNRHILTIED
PIEFVHHSKRCLINQREVHRDTHSFQAALRSALREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKSIDRI
IDVFPGSDKSMVRSMLSESLRAVVSQSLLKCISGGRVASHEIMMATPAIRNLIREDKIAQMYSMIQTGSAMGMQTMEQSV
KMLVAQGLVEAEEGRRIVDSAQQI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=970370 VUK15_RS07720 WP_005297689.1 447701..448735(+) (pilT) [Photobacterium damselae subsp. piscicida strain NH-LM2]
ATGGATATCACCGAATTATTAGACTTCAGTGTAAAACATAACGCATCGGATCTGCATCTTTCAGCAGGTGTTCCACCTAT
GATCCGTGTAGATGGTGATGTACGCAAGTTAAGTTTACCTGCATTAGAGCACTCAGAGGTTCATCGTCTGATTTTTGATA
TCATGAATGATGCCCAACGTCGTCAATTTGAAGAAGAGTTAGAAGTCGACTTCTCATTTGAATTACCCGATGTTGGCCGT
TTTCGTGTTAACGCCTTTCACCAATCTCGAGGTTGTTCTGCGGTCTTTCGTACTATCCCTATGACCATTCCAACGTTGGA
ATCTCTGAATGTGCCCGATGTTTTTTACAAAATAGCACAATGTCAGCGTGGATTGGTATTAGTTACAGGGGCTACAGGTT
CTGGTAAATCCACCACCATTGCAGCATTGGTTGATTATATTAATGAGCATACCAATCGTCATATCCTCACGATTGAAGAT
CCAATTGAATTTGTGCATCACAGTAAACGTTGTTTGATTAACCAACGAGAAGTACATCGTGATACCCACAGTTTTCAAGC
CGCTTTGCGCTCGGCATTACGTGAAGATCCCGATGTGATTGTTGTGGGGGAATTGCGAGATCAAGAGACCATCAGTTTAG
CGTTAACTGCTGCAGAAACCGGACACTTGGTTTTAGGTACGCTACACACAAGTTCAGCAGCCAAAAGTATTGACCGTATT
ATCGATGTATTTCCTGGTAGTGATAAATCCATGGTTCGCTCCATGTTATCTGAGTCATTACGTGCAGTGGTTTCCCAAAG
TTTGTTGAAATGCATTAGCGGTGGTCGTGTGGCTTCCCATGAGATCATGATGGCAACCCCTGCGATTCGAAATTTGATTC
GCGAAGATAAGATTGCTCAGATGTATTCCATGATCCAAACCGGATCGGCAATGGGAATGCAGACAATGGAACAGAGTGTG
AAGATGCTGGTTGCCCAAGGACTAGTGGAAGCAGAAGAAGGTCGTCGTATTGTCGACAGTGCCCAACAGATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.88

96.802

0.773
  pilT Vibrio cholerae strain A1552

79.88

96.802

0.773
  pilT Acinetobacter baumannii D1279779

70.448

97.384

0.686
  pilT Acinetobacter baumannii strain A118

70.448

97.384

0.686
  pilT Acinetobacter nosocomialis M2

70.149

97.384

0.683
  pilT Pseudomonas aeruginosa PAK

70.149

97.384

0.683
  pilT Pseudomonas stutzeri DSM 10701

69.851

97.384

0.68
  pilT Acinetobacter baylyi ADP1

68.421

99.419

0.68
  pilT Legionella pneumophila strain Lp02

69.605

95.64

0.666
  pilT Legionella pneumophila strain ERS1305867

69.605

95.64

0.666
  pilT Neisseria meningitidis 8013

65.579

97.965

0.642
  pilT Neisseria gonorrhoeae MS11

65.282

97.965

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

100

0.5
  pilU Vibrio cholerae strain A1552

41.159

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.13

93.605

0.366

Multiple sequence alignment