Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VUK15_RS07725 Genome accession   NZ_CP150462
Coordinates   448747..449853 (+) Length   368 a.a.
NCBI ID   WP_005297691.1    Uniprot ID   A0A2T3I981
Organism   Photobacterium damselae subsp. piscicida strain NH-LM2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443747..454853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUK15_RS07695 (VUK15_07695) - 444583..445176 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  VUK15_RS07700 (VUK15_07700) yggU 445278..445577 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  VUK15_RS07705 (VUK15_07705) - 445574..446131 (-) 558 WP_005297682.1 YggT family protein -
  VUK15_RS07710 (VUK15_07710) proC 446143..446961 (-) 819 WP_005297684.1 pyrroline-5-carboxylate reductase -
  VUK15_RS07715 (VUK15_07715) - 446977..447678 (-) 702 WP_106142333.1 YggS family pyridoxal phosphate-dependent enzyme -
  VUK15_RS07720 (VUK15_07720) pilT 447701..448735 (+) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  VUK15_RS07725 (VUK15_07725) pilU 448747..449853 (+) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VUK15_RS07730 (VUK15_07730) ruvX 450181..450606 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  VUK15_RS07735 (VUK15_07735) - 450603..451166 (-) 564 WP_106142332.1 YqgE/AlgH family protein -
  VUK15_RS07740 (VUK15_07740) gshB 451320..452267 (-) 948 WP_005297697.1 glutathione synthase -
  VUK15_RS07745 (VUK15_07745) rsmE 452288..453019 (-) 732 WP_005297699.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VUK15_RS07750 (VUK15_07750) endA 453151..453855 (-) 705 WP_005297700.1 deoxyribonuclease I -
  VUK15_RS07755 (VUK15_07755) - 453986..454468 (-) 483 WP_036763215.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41354.51 Da        Isoelectric Point: 5.5884

>NTDB_id=970371 VUK15_RS07725 WP_005297691.1 448747..449853(+) (pilU) [Photobacterium damselae subsp. piscicida strain NH-LM2]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGVLHPIGDTLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=970371 VUK15_RS07725 WP_005297691.1 448747..449853(+) (pilU) [Photobacterium damselae subsp. piscicida strain NH-LM2]
ATGAAACTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGTGTGTTGCATCCCATTGGCGATACGTTAGATCGTACAGGGGTTGATTTACTGTTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGGGGCGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGTGCAACGGGGTCGGGTA
AATCGACTTCGATGGCGGCAATGACCGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGTCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTGCGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCGGAAACGGGTCATTTATGTATGGCCACATTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGTATTCTGCAT
CTGGTACCGAAAGAGCGCCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCAGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCAGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACTAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTAACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3I981

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

99.728

0.679
  pilU Pseudomonas stutzeri DSM 10701

59.497

97.283

0.579
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

93.75

0.389
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367

Multiple sequence alignment