Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MHB44_RS06470 Genome accession   NZ_CP150292
Coordinates   1399468..1400514 (+) Length   348 a.a.
NCBI ID   WP_341302036.1    Uniprot ID   -
Organism   Lysinibacillus sp. FSL H8-0500     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1394468..1405514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB44_RS06450 (MHB44_06450) - 1395192..1395632 (-) 441 WP_341302033.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHB44_RS06455 (MHB44_06455) - 1395638..1396108 (-) 471 WP_341302034.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHB44_RS06460 (MHB44_06460) - 1396436..1397767 (+) 1332 WP_341302035.1 G5 domain-containing protein -
  MHB44_RS06465 (MHB44_06465) - 1397786..1399456 (+) 1671 WP_200908517.1 ATPase, T2SS/T4P/T4SS family -
  MHB44_RS06470 (MHB44_06470) pilT 1399468..1400514 (+) 1047 WP_341302036.1 type IV pilus twitching motility protein PilT Machinery gene
  MHB44_RS06475 (MHB44_06475) - 1400511..1401716 (+) 1206 WP_341302037.1 type II secretion system F family protein -
  MHB44_RS06480 (MHB44_06480) - 1401846..1402322 (+) 477 WP_341302038.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHB44_RS06485 (MHB44_06485) - 1402383..1402871 (+) 489 WP_341302039.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHB44_RS06490 (MHB44_06490) pilM 1402987..1403961 (+) 975 WP_341302040.1 pilus assembly protein PilM -
  MHB44_RS06495 (MHB44_06495) - 1403961..1404554 (+) 594 WP_341302041.1 hypothetical protein -
  MHB44_RS06500 (MHB44_06500) - 1404551..1405243 (+) 693 WP_341302042.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38764.56 Da        Isoelectric Point: 6.9386

>NTDB_id=969731 MHB44_RS06470 WP_341302036.1 1399468..1400514(+) (pilT) [Lysinibacillus sp. FSL H8-0500]
MTAYSIVELLTYAFEEKASDLHVTKGIPPVYRINGQLQQYGTVPLESEDLKEMIKVILPVHKQQDFEEKGETDFNFSLPG
KCRFRVNAYHQRNEVTIAARLIEAKIPSIEALNMPQVLYRLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETRSKHIIT
LEDPIEYLHTHKKSVINQREVGVDTMSFANGLRASLRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTTI
DRIIDVFPPHQQGQIRVQLANVLQGIVSQRLFVRKDVKGRVAATEILVQAPAISNLIRNEKIHQIPSVMQTSKALGMHTL
ESSMQHLIATGKISLEDARPYMNAGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=969731 MHB44_RS06470 WP_341302036.1 1399468..1400514(+) (pilT) [Lysinibacillus sp. FSL H8-0500]
ATGACCGCGTATTCAATTGTTGAGTTATTAACATATGCATTTGAGGAAAAAGCATCAGACTTACATGTTACAAAGGGTAT
ACCACCAGTATACCGTATCAATGGGCAGCTACAGCAATATGGCACAGTGCCACTAGAATCAGAAGATTTAAAGGAAATGA
TCAAGGTTATTCTACCTGTGCATAAACAACAGGATTTTGAAGAAAAGGGAGAGACGGACTTTAACTTTTCATTGCCTGGG
AAATGTCGATTCCGTGTTAATGCCTATCATCAGCGCAATGAAGTCACAATAGCCGCTCGTTTAATCGAAGCAAAAATCCC
TTCTATTGAGGCACTAAATATGCCGCAGGTGCTATACCGACTTGCGGAAAAGCCACAGGGACTTATTTTAGTAACAGGCC
CTACTGGCTCTGGTAAATCTACAACACTAGCAGCTATGATTGATTATATTAATGAAACAAGATCTAAACATATTATTACC
TTAGAGGACCCAATTGAATATTTGCATACACATAAAAAGTCAGTGATCAATCAGCGTGAGGTTGGTGTTGATACCATGAG
CTTTGCCAATGGCTTACGTGCCTCCTTGCGTCAAGACCCCGATATTATTTTAGTAGGGGAAATGCGAGACTATGAAACAA
TCTCAACAGCGATTACGGCTGCTGAAACAGGACATTTAGTAATGGCTACGCTTCATACAAGTAGTGCGCCAACGACCATT
GACCGTATTATTGATGTCTTTCCGCCACACCAGCAAGGGCAAATTCGTGTACAGCTTGCCAATGTACTACAGGGCATTGT
ATCCCAGCGTTTATTTGTGCGTAAGGATGTCAAAGGGCGTGTTGCGGCAACAGAAATTTTAGTGCAGGCGCCTGCGATTT
CTAATTTAATTCGTAATGAAAAAATCCATCAGATTCCAAGTGTAATGCAAACAAGTAAAGCATTGGGTATGCACACCTTA
GAATCCTCCATGCAGCATTTAATTGCCACAGGGAAAATTTCATTAGAGGATGCACGACCCTATATGAATGCTGGTGATTA
TAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.599

93.966

0.494
  pilT Pseudomonas aeruginosa PAK

51.198

95.977

0.491
  pilT Acinetobacter baumannii strain A118

50.599

95.977

0.486
  pilT Acinetobacter baumannii D1279779

50.599

95.977

0.486
  pilT Acinetobacter nosocomialis M2

50.299

95.977

0.483
  pilT Pseudomonas stutzeri DSM 10701

49.701

95.977

0.477
  pilT Acinetobacter baylyi ADP1

49.701

95.977

0.477
  pilT Legionella pneumophila strain ERS1305867

50.152

94.54

0.474
  pilT Legionella pneumophila strain Lp02

50.152

94.54

0.474
  pilT Neisseria meningitidis 8013

49.401

95.977

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.401

95.977

0.474
  pilT Vibrio cholerae strain A1552

49.401

95.977

0.474
  pilT Neisseria gonorrhoeae MS11

49.102

95.977

0.471
  pilU Pseudomonas stutzeri DSM 10701

42.9

95.115

0.408
  pilU Vibrio cholerae strain A1552

42.547

92.529

0.394
  pilU Acinetobacter baylyi ADP1

39.941

97.126

0.388

Multiple sequence alignment