Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OO774_RS02280 Genome accession   NZ_AP026763
Coordinates   473974..475014 (+) Length   346 a.a.
NCBI ID   WP_014233032.1    Uniprot ID   -
Organism   Vibrio sp. STUT-A11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468974..480014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO774_RS02250 (VspSTUT11_03720) - 470150..470752 (-) 603 WP_263837478.1 XTP/dITP diphosphatase -
  OO774_RS02255 (VspSTUT11_03730) - 470885..471316 (-) 432 WP_020334002.1 DUF4426 domain-containing protein -
  OO774_RS02260 (VspSTUT11_03740) yggU 471391..471681 (-) 291 WP_014233036.1 DUF167 family protein YggU -
  OO774_RS02265 (VspSTUT11_03750) - 471681..472238 (-) 558 WP_014233035.1 YggT family protein -
  OO774_RS02270 (VspSTUT11_03760) proC 472291..473109 (-) 819 WP_264904246.1 pyrroline-5-carboxylate reductase -
  OO774_RS02275 (VspSTUT11_03770) - 473232..473945 (-) 714 WP_264904248.1 YggS family pyridoxal phosphate-dependent enzyme -
  OO774_RS02280 (VspSTUT11_03780) pilT 473974..475014 (+) 1041 WP_014233032.1 type IV pilus twitching motility protein PilT Machinery gene
  OO774_RS02285 (VspSTUT11_03790) pilU 475045..476151 (+) 1107 WP_264904250.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OO774_RS02290 (VspSTUT11_03800) ruvX 476422..476847 (-) 426 WP_264904251.1 Holliday junction resolvase RuvX -
  OO774_RS02295 (VspSTUT11_03810) - 476888..477451 (-) 564 WP_264906045.1 YqgE/AlgH family protein -
  OO774_RS02300 (VspSTUT11_03820) gshB 477587..478537 (-) 951 WP_264904252.1 glutathione synthase -
  OO774_RS02305 (VspSTUT11_03830) rsmE 478552..479283 (-) 732 WP_264904253.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38247.54 Da        Isoelectric Point: 6.3867

>NTDB_id=96797 OO774_RS02280 WP_014233032.1 473974..475014(+) (pilT) [Vibrio sp. STUT-A11]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELQNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLDQLEAPEIFSKIASGQKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLSSVIAQKLLKRNGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLLAQGIVSHEEADSKVGLDVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=96797 OO774_RS02280 WP_014233032.1 473974..475014(+) (pilT) [Vibrio sp. STUT-A11]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCAGATCTACATCTTTCTGCAGGTGTACCACCTAT
GGTCAGAATTGATGGTGATGTGAGAAAGCTCGGGATTCCAGCGTTTACACACCAAGAGGTACATCGCTTGGTATTCGAAA
TCATGAATGACGCTCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAACTACAGAACGTTGGTCGC
TTTCGTGTCAACGCCTTTAACCAGTCACGTGGCTGCGCAGCGGTATTTCGTACCATTCCAAGCAGCATACCAACGCTTGA
TCAACTCGAAGCTCCAGAAATTTTCAGCAAGATTGCCAGCGGGCAAAAAGGCTTAGTTCTGGTAACAGGACCAACCGGCT
CTGGTAAATCGACGACTTTAGCTGCCATTGTCGACTACATCAATCGTAATCACAATAAGCACATTCTCACAATTGAAGAT
CCAATTGAATTTGTTCACACCAACAACAAATGTTTGATCAACCAGCGTGAAGTTCACCGTGATACTCATAGCTTCCAAAA
CGCCTTACGCAGTGCGTTGCGTGAAGACCCGGATGTCATCTTAGTTGGTGAGATGCGTGATAAAGAAACCATCAGCCTTG
CATTGACCGCCGCAGAAACCGGTCATTTGGTTTTTGGAACGCTACACACCAGCTCAGCTGCAAAAACCATCGACCGTATT
ATTGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCGATGTTGTCAGAATCGTTAAGCTCAGTGATTGCCCAGAA
GTTACTTAAGCGTAATGGTGGTGGCCGAGTCGCCTGCCACGAAATCATGATGGCGACACCAGCAATCCGTAACTTGATTC
GTGAAGATAAAGTCGCTCAGATGTATTCGATAATTCAAACTGGTGCGGCGCACGGCATGCAGACAATGGAGCAAAATGCG
CGTCAGCTATTGGCGCAAGGTATTGTTTCTCATGAAGAGGCCGACAGCAAAGTTGGGCTTGATGTACAGCAGTTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

71.856

96.532

0.694
  pilT Acinetobacter baumannii strain A118

71.856

96.532

0.694
  pilT Acinetobacter nosocomialis M2

71.557

96.532

0.691
  pilT Acinetobacter baylyi ADP1

71.818

95.376

0.685
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.399

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Legionella pneumophila strain Lp02

67.742

98.555

0.668
  pilT Legionella pneumophila strain ERS1305867

67.742

98.555

0.668
  pilT Neisseria meningitidis 8013

65.373

96.821

0.633
  pilT Neisseria gonorrhoeae MS11

65.075

96.821

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.991

100

0.491
  pilU Vibrio cholerae strain A1552

41.194

96.821

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.821

0.393
  pilU Acinetobacter baylyi ADP1

38.393

97.11

0.373

Multiple sequence alignment