Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OO774_RS02285 Genome accession   NZ_AP026763
Coordinates   475045..476151 (+) Length   368 a.a.
NCBI ID   WP_264904250.1    Uniprot ID   -
Organism   Vibrio sp. STUT-A11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470045..481151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO774_RS02250 (VspSTUT11_03720) - 470150..470752 (-) 603 WP_263837478.1 XTP/dITP diphosphatase -
  OO774_RS02255 (VspSTUT11_03730) - 470885..471316 (-) 432 WP_020334002.1 DUF4426 domain-containing protein -
  OO774_RS02260 (VspSTUT11_03740) yggU 471391..471681 (-) 291 WP_014233036.1 DUF167 family protein YggU -
  OO774_RS02265 (VspSTUT11_03750) - 471681..472238 (-) 558 WP_014233035.1 YggT family protein -
  OO774_RS02270 (VspSTUT11_03760) proC 472291..473109 (-) 819 WP_264904246.1 pyrroline-5-carboxylate reductase -
  OO774_RS02275 (VspSTUT11_03770) - 473232..473945 (-) 714 WP_264904248.1 YggS family pyridoxal phosphate-dependent enzyme -
  OO774_RS02280 (VspSTUT11_03780) pilT 473974..475014 (+) 1041 WP_014233032.1 type IV pilus twitching motility protein PilT Machinery gene
  OO774_RS02285 (VspSTUT11_03790) pilU 475045..476151 (+) 1107 WP_264904250.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OO774_RS02290 (VspSTUT11_03800) ruvX 476422..476847 (-) 426 WP_264904251.1 Holliday junction resolvase RuvX -
  OO774_RS02295 (VspSTUT11_03810) - 476888..477451 (-) 564 WP_264906045.1 YqgE/AlgH family protein -
  OO774_RS02300 (VspSTUT11_03820) gshB 477587..478537 (-) 951 WP_264904252.1 glutathione synthase -
  OO774_RS02305 (VspSTUT11_03830) rsmE 478552..479283 (-) 732 WP_264904253.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OO774_RS02310 (VspSTUT11_03840) - 479424..480119 (-) 696 WP_264904255.1 endonuclease -
  OO774_RS02315 (VspSTUT11_03850) - 480243..480740 (-) 498 WP_264904256.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41315.40 Da        Isoelectric Point: 6.4221

>NTDB_id=96798 OO774_RS02285 WP_264904250.1 475045..476151(+) (pilU) [Vibrio sp. STUT-A11]
MDLNKFLEGMLALQASDLYITVGAPILFRVDGELRPQGDKLTENDVAQLLDNAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETDIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSTKTGHILTVEDPI
EFVHEHRRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKSGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPLATGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=96798 OO774_RS02285 WP_264904250.1 475045..476151(+) (pilU) [Vibrio sp. STUT-A11]
ATGGATCTGAATAAATTTCTCGAAGGGATGCTGGCGCTGCAAGCGTCGGATCTTTATATCACGGTCGGCGCGCCGATTTT
GTTTCGTGTCGACGGTGAGTTACGTCCGCAAGGTGACAAGCTGACAGAAAATGATGTGGCGCAGTTGCTGGATAATGCAA
TGGATCCTGAGCGTCGTCAGGAGTTTCGAAAAAGTCGAGAGTCTAACTTTGCTATCGTCAGAGACTGTGGTCGATTCCGT
GTCAGTGCCTTTTTCCAGCGAGAATTGCCGGGCGCGGTTATTCGCCGTATTGAGACCGACATCCCTACCTTTGAGCAGCT
CAAACTGCCTCTGGTGTTGCAAGATTTAGCGATCGCCAAGCGAGGTCTGGTGCTGGTGGTTGGTGCGACAGGTTCTGGTA
AGTCGACCACGATGGCGGCGATGACAGGCTACCGAAACAGTACTAAGACCGGGCATATCCTGACGGTAGAAGATCCGATC
GAATTTGTTCATGAACACCGGCGTTGTATCGTAACCCAGCGTGAAGTCGGTTTAGACACCGAAAGTTACGAAGTGGCACT
GAAAAACTCCCTGCGACAGGCTCCGGATATGATTTTGATCGGCGAAATTCGCAGTAGAGAGACCATGGAATATGCGATGA
CCTTTGCTGAAACCGGGCATTTGTGTATGGCGACTCTCCACGCGAATAACGCCAACCAGGCGTTAGAACGTATTCTTCAT
TTAGTGCCTAAAGATCAAAAAGAGCAGTTCTTGTTTGATCTGTCTATGAACTTAAAAGGCGTGGTTGGCCAGCAGCTAAT
CCGCGATAAAAGTGGGCAGGGGCGTCATGGCGTATTTGAGATCCTGCTGAACAGTCCGCGTGTTTCGGATTTAATCCGTC
GAGGCGATCTTCATGAACTCAAGTCGACCATGGCTCGTTCAAATGAGTTTGGTATGCTGACTTTTGACCAGTCGCTCTAC
AAGTTAGTTATGCAAGGGAAAATCAGTGAAGAGGATGCACTGCACAGTGCAGATTCGGCAAATGACCTGCGTCTCATGCT
GAAAACTCAACGCGGAGAGCCTCTAGCAACAGGAAGCTTGGCGAACGTTAAGATCGATATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.522

100

0.815
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Pseudomonas stutzeri DSM 10701

40.643

92.935

0.378
  pilT Acinetobacter baylyi ADP1

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372

Multiple sequence alignment