Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CTR2_RS25465 Genome accession   NZ_AP026738
Coordinates   5483408..5484451 (+) Length   347 a.a.
NCBI ID   WP_087079988.1    Uniprot ID   -
Organism   Comamonas thiooxydans strain R2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5478408..5489451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTR2_RS25445 (CTR2_R49660) - 5478706..5480304 (-) 1599 WP_087079996.1 AMP-binding protein -
  CTR2_RS25450 (CTR2_R49670) - 5480470..5481240 (+) 771 WP_087079994.1 TetR/AcrR family transcriptional regulator -
  CTR2_RS25455 (CTR2_R49680) - 5481288..5482535 (+) 1248 WP_087079992.1 branched-chain amino acid ABC transporter substrate-binding protein -
  CTR2_RS25460 (CTR2_R49690) - 5482648..5483370 (-) 723 WP_087079990.1 YggS family pyridoxal phosphate-dependent enzyme -
  CTR2_RS25465 (CTR2_R49700) pilT 5483408..5484451 (+) 1044 WP_087079988.1 type IV pilus twitching motility protein PilT Machinery gene
  CTR2_RS25470 (CTR2_R49710) pilU 5484577..5485713 (+) 1137 WP_087079986.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CTR2_RS25475 (CTR2_R49720) - 5485862..5486773 (+) 912 WP_087079984.1 NAD(P)-dependent oxidoreductase -
  CTR2_RS25480 (CTR2_R49730) - 5486834..5487487 (-) 654 WP_087079982.1 BON domain-containing protein -
  CTR2_RS25485 (CTR2_R49740) - 5487484..5488092 (-) 609 WP_003050573.1 SIS domain-containing protein -
  CTR2_RS25490 (CTR2_R49750) - 5488182..5488583 (-) 402 WP_003050571.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38340.22 Da        Isoelectric Point: 6.7742

>NTDB_id=96782 CTR2_RS25465 WP_087079988.1 5483408..5484451(+) (pilT) [Comamonas thiooxydans strain R2]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKTVHAMVYDIMSDAQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSQNMGMQTLDQN
LTDLVRRNLISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=96782 CTR2_RS25465 WP_087079988.1 5483408..5484451(+) (pilT) [Comamonas thiooxydans strain R2]
GTGGATATCACGCAATTGCTGGCATTCAGTGTCAAGAACAAGGCTTCGGACTTGCATTTGTCGGCAGGCCTGCCCCCCAT
GATTCGCGTCCACGGCGATGTGCGCCGCATCAACATCGATCCGCTGGACCACAAGACCGTGCACGCCATGGTCTACGACA
TCATGAGCGATGCCCAGCGCAAGCAGTACGAAGAGTTTCTGGAAGTGGACTTTTCGTTCGAGATTGAAGGCCTGGCCCGG
TTTCGTGTCAATGCCTTCAACCAGAACCGCGGCGCGGCGGCCGTGTTCCGTACCATTCCCAGCAAGATCCTGACACTGGA
GCAGCTCAACGCCCCCAAGATCTTCGCCGACCTGGCCCTGAAGCCACGTGGTCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGCCATGGTCAACTATCTCAACGAGACCGAGTACGGCCACATCCTGACGGTGGAAGAC
CCCATCGAATTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCCATGACGCTGTCGTTTGCGGC
AGCGCTGAAGTCCGCGCTGCGTGAAGACCCGGACGCGATCCTGGTCGGCGAAATGCGGGACCTGGAAACCATCCGTCTGG
CCATGACGGCGGCCGAAACCGGTCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCAGCCAAGACCATCGACCGTATC
ATTGACGTGTTCCCGGCCGAGGAAAAGGAAATGGTGCGTGCCATGCTGTCCGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGTGTGGCCGCACACGAGATCATGCTGGGCACGCCCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCACAGATGTACTCCACCATTCAGACCAGCCAGAACATGGGCATGCAGACTCTTGACCAGAAC
CTGACCGATCTGGTGCGCCGCAACCTCATCAGCCCGGCCGAAGCGCGCAGCAAGGCGAAGATTCCCGAAAACTTTCCTGG
ATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409

Multiple sequence alignment