Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CTR2_RS25470 Genome accession   NZ_AP026738
Coordinates   5484577..5485713 (+) Length   378 a.a.
NCBI ID   WP_087079986.1    Uniprot ID   -
Organism   Comamonas thiooxydans strain R2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5479577..5490713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTR2_RS25450 (CTR2_R49670) - 5480470..5481240 (+) 771 WP_087079994.1 TetR/AcrR family transcriptional regulator -
  CTR2_RS25455 (CTR2_R49680) - 5481288..5482535 (+) 1248 WP_087079992.1 branched-chain amino acid ABC transporter substrate-binding protein -
  CTR2_RS25460 (CTR2_R49690) - 5482648..5483370 (-) 723 WP_087079990.1 YggS family pyridoxal phosphate-dependent enzyme -
  CTR2_RS25465 (CTR2_R49700) pilT 5483408..5484451 (+) 1044 WP_087079988.1 type IV pilus twitching motility protein PilT Machinery gene
  CTR2_RS25470 (CTR2_R49710) pilU 5484577..5485713 (+) 1137 WP_087079986.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CTR2_RS25475 (CTR2_R49720) - 5485862..5486773 (+) 912 WP_087079984.1 NAD(P)-dependent oxidoreductase -
  CTR2_RS25480 (CTR2_R49730) - 5486834..5487487 (-) 654 WP_087079982.1 BON domain-containing protein -
  CTR2_RS25485 (CTR2_R49740) - 5487484..5488092 (-) 609 WP_003050573.1 SIS domain-containing protein -
  CTR2_RS25490 (CTR2_R49750) - 5488182..5488583 (-) 402 WP_003050571.1 YraN family protein -
  CTR2_RS25495 (CTR2_R49760) rsmI 5488583..5489506 (+) 924 WP_087079980.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42091.55 Da        Isoelectric Point: 6.7687

>NTDB_id=96783 CTR2_RS25470 WP_087079986.1 5484577..5485713(+) (pilU) [Comamonas thiooxydans strain R2]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGKVTKVSPQPLNQLHTLALARSIMNDRQVADFEHTKECNFAI
SPAGVGRFRVNAFMQQGKVGMVLRTIPTTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTESWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDVSLNLRAIISQRLIPKQDSKGRVAAIEVMLNSPLIADLIFKGEVVEIKEIMKKSRNIG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLNSQRARTQDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=96783 CTR2_RS25470 WP_087079986.1 5484577..5485713(+) (pilU) [Comamonas thiooxydans strain R2]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACTGCAGAGTTTCCGCCCGCCATCAAGATTGACGGCAAAGTCACCAAGGTCTCGCCCCAGCCGCTGAACCAGTTGCATA
CGCTGGCCCTGGCGCGTTCCATCATGAACGACAGGCAGGTGGCAGACTTCGAACACACCAAGGAGTGCAACTTCGCCATC
TCGCCGGCCGGTGTGGGGCGCTTCCGTGTCAACGCCTTCATGCAGCAGGGCAAGGTCGGCATGGTGCTGCGTACCATTCC
CACCACCCTGCCCACGATTGACGGCCTGGGCGTGCCGCAGGTGCTCAAGGAAGTGACCATGACCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCGACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTGGACTGGCGCAATGAAAACTCCTTCGGC
CACATCATCACCGTGGAAGATCCCGTGGAATTCGTGCATCCGCACAAGAACTGCGTGGTCACGCAACGCGAGGTGGGACT
GGACACGGAAAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGATGTGATTCTGATGGGCGAAATCCGTG
ACCGCGAAACCATGGAGCACGCGATTGCGTTCTCTGAAACCGGTCACCTGTGTCTGGCCACGCTGCACGCCAACAGTGCC
AACCAGGCACTGGACCGTATCGTCAACTTCTTCCCCGAAGAGCGCCGTCCGCAACTGCTGATGGATGTGTCGCTGAATCT
GCGGGCCATCATTTCCCAGCGCCTGATCCCCAAGCAGGACAGCAAGGGCCGTGTCGCCGCCATCGAGGTGATGCTCAACT
CGCCGCTGATCGCAGACCTGATCTTCAAGGGCGAGGTCGTCGAGATCAAGGAGATCATGAAGAAAAGCCGCAATATCGGC
ATGCAGACCTTTGACCAGGCGCTGTTCGACCTGTTCGAATCCAACCTCATCACCTATGAAGATGCGCTGCGCAATGCAGA
CTCGGTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCCAGCGCGCCCGGACTCAGGACCTGGCGGCCGGCACCGAAC
ATCTGACCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.929

96.825

0.59
  pilU Acinetobacter baylyi ADP1

55.71

94.974

0.529
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362
  pilT Acinetobacter baylyi ADP1

41.768

86.772

0.362

Multiple sequence alignment