Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKY51_RS01335 Genome accession   NZ_CP150231
Coordinates   261648..262691 (+) Length   347 a.a.
NCBI ID   WP_339174834.1    Uniprot ID   -
Organism   Solibacillus sp. FSL R5-0691     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 256648..267691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY51_RS01315 (MKY51_01315) - 257238..257684 (-) 447 WP_339174829.1 type II secretion system protein -
  MKY51_RS01320 (MKY51_01320) - 257681..258142 (-) 462 WP_339174830.1 hypothetical protein -
  MKY51_RS01325 (MKY51_01325) - 258576..259955 (+) 1380 WP_339174831.1 VanW family protein -
  MKY51_RS01330 (MKY51_01330) - 259971..261635 (+) 1665 WP_339174833.1 GspE/PulE family protein -
  MKY51_RS01335 (MKY51_01335) pilT 261648..262691 (+) 1044 WP_339174834.1 type IV pilus twitching motility protein PilT Machinery gene
  MKY51_RS01340 (MKY51_01340) - 262688..263896 (+) 1209 WP_339174836.1 type II secretion system F family protein -
  MKY51_RS01345 (MKY51_01345) - 263993..264418 (+) 426 WP_339174838.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKY51_RS01350 (MKY51_01350) pilM 264544..265521 (+) 978 WP_339174839.1 pilus assembly protein PilM -
  MKY51_RS01355 (MKY51_01355) - 265518..266108 (+) 591 WP_339174840.1 PilN domain-containing protein -
  MKY51_RS01360 (MKY51_01360) - 266108..266818 (+) 711 WP_339174841.1 potassium transporter -
  MKY51_RS01365 (MKY51_01365) - 266851..267603 (+) 753 WP_339174842.1 prepilin peptidase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38270.73 Da        Isoelectric Point: 6.6391

>NTDB_id=967080 MKY51_RS01335 WP_339174834.1 261648..262691(+) (pilT) [Solibacillus sp. FSL R5-0691]
MTLNIQDLLQHAYDENASDLHVTTGIPPVYRVNGQLKQYGNVAVTAEMIEQMVNDILPAHKKAEFEEKGETDFNYSLKGF
CRFRVNAYHQRNVGAIAARLISSEIPTIDSLNMPKVLYDLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETKSKHIITL
EDPIEYLHSHKKSVVNQREIGVDTGSFANGLRASLRQDPDIILVGEMRDLETISTAITAAETGHLVFGTLHTSSAPTTID
RIIDVFPPHQQGQIRIQLANVLQGIISQRLFIRKDKAGRVAATEILVSIPAVTNLIRNEKIHQIPSVMQTNRALGMHTLE
TSIQALVSSGKVSLEEVRPYLNVGDYS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=967080 MKY51_RS01335 WP_339174834.1 261648..262691(+) (pilT) [Solibacillus sp. FSL R5-0691]
ATGACATTGAACATACAAGATTTACTGCAGCATGCGTATGATGAAAATGCTTCCGATTTGCATGTCACTACGGGAATCCC
TCCTGTTTACCGAGTAAATGGTCAGTTGAAGCAATATGGCAATGTAGCTGTGACAGCTGAAATGATTGAACAAATGGTCA
ATGATATTTTACCTGCTCACAAAAAAGCAGAGTTTGAAGAAAAGGGCGAAACTGATTTCAACTATTCATTGAAAGGTTTT
TGCCGTTTCCGTGTAAATGCCTATCATCAGCGAAATGTTGGGGCAATCGCTGCCCGTCTTATTTCGAGTGAAATCCCGAC
GATTGACTCGTTGAATATGCCGAAAGTACTGTACGATCTAGCTGAAAAACCTCAAGGGCTCATTTTAGTAACGGGTCCGA
CCGGTTCAGGGAAGTCAACAACACTTGCGGCAATGATTGACTATATTAATGAAACAAAATCAAAGCATATTATCACGCTG
GAGGACCCGATCGAATATTTGCATTCCCATAAAAAATCGGTAGTGAATCAGCGTGAAATCGGAGTCGATACCGGGTCATT
TGCGAATGGTCTGCGTGCATCGCTCCGTCAAGATCCAGATATTATTCTAGTAGGGGAAATGCGAGATTTAGAGACTATTT
CAACAGCGATTACGGCAGCAGAAACAGGCCACTTAGTATTCGGTACACTTCATACTTCAAGTGCACCGACAACAATTGAC
CGCATTATCGATGTGTTCCCGCCGCATCAGCAAGGGCAGATCCGGATTCAGTTAGCGAATGTATTGCAAGGGATTATTTC
ACAGCGATTATTCATTCGTAAAGATAAAGCGGGCCGGGTTGCCGCGACAGAAATTCTCGTAAGTATTCCGGCTGTCACAA
ATTTGATAAGAAATGAAAAAATTCATCAAATTCCGAGTGTGATGCAGACAAATCGCGCGTTAGGTATGCATACATTAGAG
ACATCCATACAAGCGCTTGTTTCTTCCGGTAAAGTTTCATTGGAAGAAGTTCGCCCATATTTGAATGTGGGTGATTACAG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.308

93.66

0.49
  pilT Pseudomonas aeruginosa PAK

50.445

97.118

0.49
  pilT Acinetobacter nosocomialis M2

50.148

97.118

0.487
  pilT Acinetobacter baylyi ADP1

49.852

97.118

0.484
  pilT Acinetobacter baumannii D1279779

49.852

97.118

0.484
  pilT Acinetobacter baumannii strain A118

49.852

97.118

0.484
  pilT Neisseria meningitidis 8013

50

96.83

0.484
  pilT Pseudomonas stutzeri DSM 10701

49.555

97.118

0.481
  pilT Neisseria gonorrhoeae MS11

49.702

96.83

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.81

96.83

0.473
  pilT Vibrio cholerae strain A1552

48.81

96.83

0.473
  pilT Legionella pneumophila strain Lp02

48.943

95.389

0.467
  pilT Legionella pneumophila strain ERS1305867

48.943

95.389

0.467
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.254

0.389
  pilU Vibrio cholerae strain A1552

41.121

92.507

0.38
  pilU Acinetobacter baylyi ADP1

38.253

95.677

0.366

Multiple sequence alignment