Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NSQ23_RS04315 Genome accession   NZ_CP150179
Coordinates   823927..824961 (+) Length   344 a.a.
NCBI ID   WP_339173243.1    Uniprot ID   -
Organism   Anoxybacillus sp. FSL W8-1294     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 818927..829961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ23_RS04295 (NSQ23_04295) - 819110..820234 (+) 1125 WP_339173235.1 hypothetical protein -
  NSQ23_RS04300 (NSQ23_04300) - 820231..820977 (+) 747 WP_064214444.1 PRC-barrel domain-containing protein -
  NSQ23_RS04305 (NSQ23_04305) - 820991..822253 (+) 1263 WP_339173237.1 VanW family protein -
  NSQ23_RS04310 (NSQ23_04310) - 822250..823911 (+) 1662 WP_339173240.1 ATPase, T2SS/T4P/T4SS family -
  NSQ23_RS04315 (NSQ23_04315) pilT 823927..824961 (+) 1035 WP_339173243.1 type IV pilus twitching motility protein PilT Machinery gene
  NSQ23_RS04320 (NSQ23_04320) - 824968..826179 (+) 1212 WP_339173245.1 type II secretion system F family protein -
  NSQ23_RS04325 (NSQ23_04325) - 826265..826783 (+) 519 WP_064214441.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NSQ23_RS04330 (NSQ23_04330) comC 826855..827604 (+) 750 WP_339173248.1 A24 family peptidase Machinery gene
  NSQ23_RS04335 (NSQ23_04335) pilM 827618..828586 (+) 969 WP_339173251.1 pilus assembly protein PilM -
  NSQ23_RS04340 (NSQ23_04340) - 828570..829151 (+) 582 WP_339173254.1 PilN domain-containing protein -
  NSQ23_RS04345 (NSQ23_04345) pilO 829148..829774 (+) 627 WP_339173256.1 type 4a pilus biogenesis protein PilO -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38074.00 Da        Isoelectric Point: 7.2227

>NTDB_id=965169 NSQ23_RS04315 WP_339173243.1 823927..824961(+) (pilT) [Anoxybacillus sp. FSL W8-1294]
MKQKVDAMLRAAFELKASDIHLTVGIPPIFRINGDLKRYGQDVLSPTDTEQMAKAIVPEHMWTRFETDGELDLSYSLSGV
SRFRVNVFKQRGCISLAIRIVPTKIPTLDELQLPDVLKKMVTKPQGLILVTGPTGSGKSTTLAAMIDYMNKTMRKHIITL
EDPIEYVHKHGSCIIDQREVGTDTSSFANGLRAALRQDPDVILVGEMRDLETIQTAVTAAETGHLVFGTLHTSSAPATID
RIIDVFQPEQQAQIRIQLATVLVSIISQRLFPRAQNNGRIAATEILINNAAVANLIRNGKIHQIPSVMQTNRALGMHTLE
ANIRELVEQGLIARETVEPYLQEG

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=965169 NSQ23_RS04315 WP_339173243.1 823927..824961(+) (pilT) [Anoxybacillus sp. FSL W8-1294]
ATGAAACAAAAAGTAGATGCGATGTTGCGTGCTGCATTTGAATTAAAAGCATCTGATATTCATTTAACGGTCGGTATTCC
TCCGATTTTTCGTATTAACGGAGATTTGAAACGATATGGGCAAGATGTGTTGTCGCCAACCGATACAGAACAAATGGCAA
AAGCGATCGTTCCAGAGCATATGTGGACGCGCTTTGAAACAGACGGCGAATTAGATTTGTCATATAGTCTTTCAGGTGTG
TCACGTTTTCGTGTGAACGTATTTAAACAACGTGGATGTATATCGTTAGCCATTCGTATCGTACCGACGAAAATTCCAAC
GCTTGATGAACTGCAATTGCCGGACGTATTAAAAAAGATGGTGACGAAACCACAAGGGCTCATTCTCGTCACGGGGCCGA
CAGGGAGCGGAAAATCGACGACGTTAGCCGCGATGATCGATTATATGAACAAGACGATGCGCAAACATATTATTACGCTT
GAAGATCCGATTGAATATGTGCATAAGCATGGCAGCTGCATCATTGATCAGCGCGAAGTCGGAACGGATACGAGCAGCTT
TGCAAACGGGTTGCGCGCTGCTCTTCGCCAAGACCCTGATGTCATTTTAGTCGGAGAAATGCGCGATTTAGAAACGATTC
AAACGGCAGTGACTGCTGCTGAAACGGGGCATCTTGTTTTCGGGACGCTTCATACGTCAAGTGCCCCTGCGACGATTGAC
CGAATTATTGATGTATTTCAACCAGAACAACAGGCACAAATTCGCATTCAGTTGGCGACAGTTTTAGTTTCCATTATTTC
ACAACGACTATTTCCGCGCGCCCAAAACAACGGGCGGATTGCCGCGACGGAAATTTTAATAAACAATGCAGCAGTCGCCA
ATTTAATTCGCAACGGGAAAATTCATCAAATTCCGAGCGTCATGCAAACGAACCGTGCGCTTGGCATGCATACACTTGAG
GCGAACATTAGAGAGCTTGTCGAACAAGGACTGATTGCAAGAGAAACGGTTGAGCCGTACTTGCAGGAAGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.462

94.477

0.515
  pilT Pseudomonas aeruginosa PAK

50.613

94.767

0.48
  pilT Vibrio cholerae strain A1552

48.512

97.674

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.512

97.674

0.474
  pilT Neisseria meningitidis 8013

50.625

93.023

0.471
  pilT Neisseria gonorrhoeae MS11

50.313

93.023

0.468
  pilT Pseudomonas stutzeri DSM 10701

48.328

95.64

0.462
  pilT Acinetobacter baumannii D1279779

48.012

95.058

0.456
  pilT Acinetobacter baumannii strain A118

48.012

95.058

0.456
  pilT Acinetobacter nosocomialis M2

48.012

95.058

0.456
  pilT Acinetobacter baylyi ADP1

47.692

94.477

0.451
  pilT Legionella pneumophila strain ERS1305867

47.352

93.314

0.442
  pilT Legionella pneumophila strain Lp02

47.352

93.314

0.442
  pilU Pseudomonas stutzeri DSM 10701

42.169

96.512

0.407
  pilU Vibrio cholerae strain A1552

40.367

95.058

0.384
  pilU Acinetobacter baylyi ADP1

40.752

92.733

0.378

Multiple sequence alignment