Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OO986_RS02620 Genome accession   NZ_AP026552
Coordinates   547865..548971 (+) Length   368 a.a.
NCBI ID   WP_011079463.1    Uniprot ID   A0A3Q0L3V4
Organism   Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 542865..553971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO986_RS02585 (VV208B2_04510) - 543245..543847 (-) 603 WP_072603529.1 XTP/dITP diphosphatase -
  OO986_RS02590 (VV208B2_04520) - 543858..544289 (-) 432 WP_108693968.1 DUF4426 domain-containing protein -
  OO986_RS02595 (VV208B2_04530) yggU 544332..544622 (-) 291 WP_011151199.1 DUF167 family protein YggU -
  OO986_RS02600 (VV208B2_04540) - 544622..545179 (-) 558 WP_013571095.1 YggT family protein -
  OO986_RS02605 (VV208B2_04550) proC 545221..546039 (-) 819 WP_108693967.1 pyrroline-5-carboxylate reductase -
  OO986_RS02610 (VV208B2_04560) - 546074..546784 (-) 711 WP_011079461.1 YggS family pyridoxal phosphate-dependent enzyme -
  OO986_RS02615 (VV208B2_04570) pilT 546809..547846 (+) 1038 WP_011079462.1 type IV pilus twitching motility protein PilT Machinery gene
  OO986_RS02620 (VV208B2_04580) pilU 547865..548971 (+) 1107 WP_011079463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OO986_RS02625 (VV208B2_04590) ruvX 549064..549486 (-) 423 WP_011079464.1 Holliday junction resolvase RuvX -
  OO986_RS02630 (VV208B2_04600) - 549508..550071 (-) 564 WP_011079465.1 YqgE/AlgH family protein -
  OO986_RS02635 (VV208B2_04610) gshB 550126..551079 (-) 954 WP_108693966.1 glutathione synthase -
  OO986_RS02640 (VV208B2_04620) rsmE 551094..551825 (-) 732 WP_011079467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OO986_RS02645 (VV208B2_04630) - 552063..553397 (+) 1335 WP_043921085.1 hypothetical protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41245.35 Da        Isoelectric Point: 6.7296

>NTDB_id=96359 OO986_RS02620 WP_011079463.1 547865..548971(+) (pilU) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
MDLNKILEGMITLKASDLYITVGAPVLFRVDGELRPQGEKLSQIEVAQLLDGAMDEERRADFRKSRESNFAIVRDSGRFR
VSAFYQRELPGAVIRRIETNIPTFEELKLPHVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEVLLNSPRVSDLIRRGDLHELKMTMAKSKEVGMQTFDQSLY
TLVVEGKISEEDAMHSADSANDLRLMLKTQRGDISSPGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=96359 OO986_RS02620 WP_011079463.1 547865..548971(+) (pilU) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
ATGGATTTAAACAAGATTTTAGAAGGGATGATCACGCTTAAAGCGTCGGATCTTTATATCACGGTTGGTGCCCCCGTGCT
GTTTCGTGTCGATGGCGAGCTGCGCCCGCAAGGCGAAAAATTATCGCAAATTGAAGTCGCTCAACTGCTGGATGGGGCGA
TGGACGAAGAGCGGCGCGCTGACTTTCGCAAAAGCCGCGAGTCAAACTTTGCCATTGTGCGAGATTCGGGGCGTTTTCGT
GTCAGTGCCTTCTACCAACGAGAGCTTCCTGGTGCGGTGATTCGCCGCATTGAAACCAACATTCCAACCTTTGAGGAACT
TAAGCTGCCTCATGTTTTGCAAGACTTAGCGATTGCCAAACGTGGATTGGTGCTGGTGGTGGGCGCGACGGGCTCTGGTA
AATCGACCACCATGGCTGCGATGACGGGATATCGAAATACCCACCGTAGTGGACACATTTTGACGGTGGAAGATCCGATT
GAATTTGTCCACGAACATAAACGCTGTATTGTCACCCAGCGTGAAGTCGGTTTGGATACGGAAAGCTATGAAGTCGCACT
AAAGAACTCGCTGCGTCAAGCGCCAGATATGATTCTAATTGGTGAGATTCGCAGTCGGGAAACCATGGAATACGCCATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCGACACTGCATGCCAATAACGCCAACCAAGCACTGGAGCGCATCTTACAC
TTGGTGCCTAAAGAACAAAAAGAGCAGTTTTTGTTTGACCTTTCCATGAACTTAAAAGGGGTAGTTGGTCAGCAGTTGAT
TCGTGATAAAAATGGTAAAGGTCGTCACGGTGTGTTCGAGGTGTTACTTAACAGTCCGCGCGTGTCGGATTTGATCCGTC
GTGGCGATTTGCATGAGCTGAAAATGACTATGGCGAAGTCGAAAGAAGTCGGTATGCAGACCTTTGACCAATCACTCTAT
ACATTAGTAGTGGAAGGCAAAATCAGCGAAGAAGACGCCATGCACAGTGCCGATTCCGCCAACGATTTACGTTTGATGCT
GAAGACGCAGCGAGGTGATATTAGTTCCCCCGGCTCATTGGCGAACGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3V4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

100

0.842
  pilU Pseudomonas stutzeri DSM 10701

59.429

95.109

0.565
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.753

88.043

0.394
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.761

0.361
  pilT Vibrio cholerae strain A1552

39.82

90.761

0.361

Multiple sequence alignment