Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OO986_RS02615 Genome accession   NZ_AP026552
Coordinates   546809..547846 (+) Length   345 a.a.
NCBI ID   WP_011079462.1    Uniprot ID   A0A3Q0L3W1
Organism   Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541809..552846
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO986_RS02580 (VV208B2_04500) hemW 542066..543235 (-) 1170 WP_264922341.1 radical SAM family heme chaperone HemW -
  OO986_RS02585 (VV208B2_04510) - 543245..543847 (-) 603 WP_072603529.1 XTP/dITP diphosphatase -
  OO986_RS02590 (VV208B2_04520) - 543858..544289 (-) 432 WP_108693968.1 DUF4426 domain-containing protein -
  OO986_RS02595 (VV208B2_04530) yggU 544332..544622 (-) 291 WP_011151199.1 DUF167 family protein YggU -
  OO986_RS02600 (VV208B2_04540) - 544622..545179 (-) 558 WP_013571095.1 YggT family protein -
  OO986_RS02605 (VV208B2_04550) proC 545221..546039 (-) 819 WP_108693967.1 pyrroline-5-carboxylate reductase -
  OO986_RS02610 (VV208B2_04560) - 546074..546784 (-) 711 WP_011079461.1 YggS family pyridoxal phosphate-dependent enzyme -
  OO986_RS02615 (VV208B2_04570) pilT 546809..547846 (+) 1038 WP_011079462.1 type IV pilus twitching motility protein PilT Machinery gene
  OO986_RS02620 (VV208B2_04580) pilU 547865..548971 (+) 1107 WP_011079463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OO986_RS02625 (VV208B2_04590) ruvX 549064..549486 (-) 423 WP_011079464.1 Holliday junction resolvase RuvX -
  OO986_RS02630 (VV208B2_04600) - 549508..550071 (-) 564 WP_011079465.1 YqgE/AlgH family protein -
  OO986_RS02635 (VV208B2_04610) gshB 550126..551079 (-) 954 WP_108693966.1 glutathione synthase -
  OO986_RS02640 (VV208B2_04620) rsmE 551094..551825 (-) 732 WP_011079467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38515.16 Da        Isoelectric Point: 6.3696

>NTDB_id=96358 OO986_RS02615 WP_011079462.1 546809..547846(+) (pilT) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGVPAFNHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTNIPTLEELECPQIFEKIAKCEKGLVLVTGPTGSGKSTTLAAMVDYINRNYNKHILTIED
PIEFVHQNQKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLIAQGKVAKEEVDAKIEIETIQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=96358 OO986_RS02615 WP_011079462.1 546809..547846(+) (pilT) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAACGCATCAGATCTACATCTTTCTGCGGGTGTGCCTCCTAT
GGTACGCATAGATGGCGATGTAAGAAAGCTCGGTGTGCCCGCATTTAATCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTTGAAGAGAAGTTGGAAGTCGATTTTTCCTTTGAATTGCCTAACGTAGGTCGT
TTTCGTGTTAACGCCTTCAACCAATCTCGCGGCTGCTCTGCGGTGTTTCGTACCATTCCAACCAATATCCCTACCTTGGA
AGAGCTTGAGTGCCCGCAAATTTTTGAAAAAATTGCGAAGTGTGAAAAGGGCTTGGTGTTGGTCACTGGGCCAACAGGTT
CGGGTAAATCGACCACGTTAGCGGCGATGGTGGATTACATTAACCGCAACTACAATAAACACATCCTGACCATTGAAGAT
CCGATTGAATTTGTTCACCAAAATCAGAAATGTTTGATTAACCAACGCGAAGTTCATCGCGACACCCACAGTTTTAAAAA
TGCCCTGCGCAGCGCATTGCGTGAGGACCCAGACGTTATCTTGGTGGGTGAGTTACGTGACCAAGAGACCATCAGCCTAG
CGCTTACCGCGGCAGAAACGGGTCACTTGGTGTTTGGTACGTTGCACACCAGTAGTGCAGCAAAAACCATTGACCGTATT
ATCGATGTGTTCCCTGGCAGTGACAAAGACATGGTGCGCTCAATGCTTTCTGAATCATTGCGCGCCGTTATCGCACAGAA
ATTGCTGAAACGCATTGGCGGTGGGCGAGTGGCGTGTCATGAGATCATGATGGCAACGCCAGCCATTCGTAACTTGATCC
GTGAAGACAAAGTGGCACAAATGTACTCCATTATCCAAACGGGGGCTGCGCACGGCATGCAGACGATGGAACAGAATGCG
CGTCAGTTGATTGCACAGGGCAAAGTGGCCAAAGAAGAAGTCGACGCCAAAATTGAGATTGAAACTATCCAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3W1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.406

100

0.884
  pilT Vibrio cholerae strain A1552

88.406

100

0.884
  pilT Acinetobacter baumannii D1279779

72.892

96.232

0.701
  pilT Acinetobacter baumannii strain A118

72.892

96.232

0.701
  pilT Acinetobacter nosocomialis M2

72.59

96.232

0.699
  pilT Acinetobacter baylyi ADP1

72.477

94.783

0.687
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

68.58

95.942

0.658
  pilT Legionella pneumophila strain ERS1305867

68.58

95.942

0.658
  pilT Neisseria meningitidis 8013

66.066

96.522

0.638
  pilT Neisseria gonorrhoeae MS11

65.766

96.522

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.895

99.42

0.516
  pilU Pseudomonas stutzeri DSM 10701

42.515

96.812

0.412
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment