Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WKR14_RS15045 Genome accession   NZ_CP149966
Coordinates   3329019..3330056 (-) Length   345 a.a.
NCBI ID   WP_025009461.1    Uniprot ID   A0A1S2TIJ8
Organism   Shewanella chilikensis strain ST10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3324019..3335056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKR14_RS15020 (WKR14_15020) - 3324022..3324756 (-) 735 WP_165565430.1 tryptophan 2,3-dioxygenase family protein -
  WKR14_RS15025 (WKR14_15025) hemH 3324929..3325942 (-) 1014 WP_339086709.1 ferrochelatase -
  WKR14_RS15030 (WKR14_15030) - 3325977..3326468 (-) 492 WP_286996502.1 glutathione peroxidase -
  WKR14_RS15035 (WKR14_15035) - 3326631..3327740 (-) 1110 WP_101055500.1 hypothetical protein -
  WKR14_RS15040 (WKR14_15040) pilU 3327897..3329009 (-) 1113 WP_101055493.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WKR14_RS15045 (WKR14_15045) pilT 3329019..3330056 (-) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  WKR14_RS15050 (WKR14_15050) - 3330093..3330788 (+) 696 WP_165565434.1 YggS family pyridoxal phosphate-dependent enzyme -
  WKR14_RS15055 (WKR14_15055) proC 3330921..3331739 (+) 819 WP_339086712.1 pyrroline-5-carboxylate reductase -
  WKR14_RS15060 (WKR14_15060) - 3331763..3332314 (+) 552 WP_339086714.1 YggT family protein -
  WKR14_RS15065 (WKR14_15065) yggU 3332314..3332601 (+) 288 WP_037436422.1 DUF167 family protein YggU -
  WKR14_RS15070 (WKR14_15070) - 3332664..3333098 (+) 435 WP_101055484.1 DUF4426 domain-containing protein -
  WKR14_RS15075 (WKR14_15075) rdgB 3333319..3333921 (+) 603 WP_101055482.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38371.97 Da        Isoelectric Point: 6.8739

>NTDB_id=962399 WKR14_RS15045 WP_025009461.1 3329019..3330056(-) (pilT) [Shewanella chilikensis strain ST10]
MEITELLAFSVKHNASDLHLSAGVPPMIRVDGEVRKINVPALDHQGVHRLVYDIMNDKQRKDYEEFLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILTLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQSL
QNLVNRGLISREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=962399 WKR14_RS15045 WP_025009461.1 3329019..3330056(-) (pilT) [Shewanella chilikensis strain ST10]
ATGGAAATCACTGAATTATTGGCCTTTAGTGTAAAGCATAACGCTTCGGATCTGCACTTATCGGCCGGTGTGCCTCCCAT
GATACGCGTCGACGGTGAGGTCAGAAAGATTAATGTCCCTGCGCTTGATCACCAAGGGGTTCACAGACTTGTCTACGACA
TAATGAATGACAAGCAGCGTAAGGATTATGAAGAGTTTCTGGAAATCGACTTTTCCTTTGAAGTTCCCAATCTGGCGCGT
TTTCGTGTCAATGCCTTCAATCAGTCCCGCGGCGCCGCCGCGGTTTTTCGTACTATTCCCAGCGACATTTTGACTTTGGA
ACAACTGGGCGCGCCGGAAATCTTCAAGAAAATTGCTTCATATCCTCGCGGTTTGGTGTTGGTAACCGGCCCCACAGGTT
CGGGTAAGAGTACCACCCTGGCGGCGATGATAGATTACGTCAATGAGAACCGCCACGATCATATTCTCACCATTGAAGAC
CCTATCGAATTTGTGCACCAAAACAAGCAATGCCTTATCAACCAGCGGGAAGTGCACCGCCATACCCACAGCTTTAACGC
CGCCCTCAGAAGCGCCCTGCGTGAAGACCCGGATGTCATCCTGGTCGGTGAGATGCGGGATTTGGAAACCATACGCCTGG
CGATGACCGCGGCAGAAACCGGCCACTTGGTTTTCGGTACCTTGCACACCACCTCGGCGGCCAAGACCATTGACCGTGTG
GTTGACGTATTCCCCGCAGGCGAGAAAGACATGGTGCGTACCATGTTGTCTGAGTCGTTGCAGGCGGTTATCTCCCAGAC
TCTGATCAAGAAAGTTGGCGGCGGCCGGGTGGCGGCCCATGAGATCATGATGGGAACCCCGGCTATCCGAAACCTTATCC
GTGAGGACAAGGTGGCACAGATGTATTCTGCTATTCAAACCGGTATGGCCCATGGCATGCAAACCTTGGAGCAGAGCCTG
CAAAACCTGGTGAATCGCGGCTTGATCAGTCGGGAAGATGCCATGGCCAAGAGCTCCAACAAACAAGCGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2TIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.871

99.13

0.812
  pilT Acinetobacter baumannii D1279779

81.871

99.13

0.812
  pilT Acinetobacter baumannii strain A118

81.871

99.13

0.812
  pilT Acinetobacter baylyi ADP1

78.426

99.42

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.971

100

0.78
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.074

97.681

0.733
  pilT Vibrio cholerae strain A1552

75.074

97.681

0.733
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.857

99.42

0.426
  pilU Vibrio cholerae strain A1552

41.983

99.42

0.417
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391