Detailed information NTG0962398

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WKR14_RS15040
• Genome accession: NZ_CP149966
• Coordinates: 3327897..3329009 (-)
• Length: 370 a.a.
• NCBI ID: WP_101055493.1
• Uniprot ID: -
• Organism: Shewanella chilikensis strain ST10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3322897..3334009

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKR14_RS15020 (WKR14_15020)	-	3324022..3324756 (-)	735	WP_165565430.1	tryptophan 2,3-dioxygenase family protein	-
WKR14_RS15025 (WKR14_15025)	hemH	3324929..3325942 (-)	1014	WP_339086709.1	ferrochelatase	-
WKR14_RS15030 (WKR14_15030)	-	3325977..3326468 (-)	492	WP_286996502.1	glutathione peroxidase	-
WKR14_RS15035 (WKR14_15035)	-	3326631..3327740 (-)	1110	WP_101055500.1	hypothetical protein	-
WKR14_RS15040 (WKR14_15040)	pilU	3327897..3329009 (-)	1113	WP_101055493.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
WKR14_RS15045 (WKR14_15045)	pilT	3329019..3330056 (-)	1038	WP_025009461.1	type IV pilus twitching motility protein PilT	Machinery gene
WKR14_RS15050 (WKR14_15050)	-	3330093..3330788 (+)	696	WP_165565434.1	YggS family pyridoxal phosphate-dependent enzyme	-
WKR14_RS15055 (WKR14_15055)	proC	3330921..3331739 (+)	819	WP_339086712.1	pyrroline-5-carboxylate reductase	-
WKR14_RS15060 (WKR14_15060)	-	3331763..3332314 (+)	552	WP_339086714.1	YggT family protein	-
WKR14_RS15065 (WKR14_15065)	yggU	3332314..3332601 (+)	288	WP_037436422.1	DUF167 family protein YggU	-
WKR14_RS15070 (WKR14_15070)	-	3332664..3333098 (+)	435	WP_101055484.1	DUF4426 domain-containing protein	-
WKR14_RS15075 (WKR14_15075)	rdgB	3333319..3333921 (+)	603	WP_101055482.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-

Sequence

Protein

• Length: 370 a.a.
• Molecular weight: 41069.17 Da
• Isoelectric Point: 6.3711

>NTDB_id=962398 WKR14_RS15040 WP_101055493.1 3327897..3329009(-) (pilU) [Shewanella chilikensis strain ST10]
MDVRPFLKAMVERKASDLFITAAFPPSAKVDGELRPLAENAFTPAQSLEFVESLMTPEQKKEFHETRECNFAVGAKELGR
FRVSAFWQRESAGCVMRRIETKIPTVEQLYLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNANARGHILTIED
PVEFVHQHGKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGTGRRAAIEVLINTPRVASLIQKNELHVLKETMAKSNEQGMQTFDQA
LFKLYCEGEISYADALHHADSPNDLRLMIKLHSNDTGNSGFMEGVTLDMD

Nucleotide

• Length: 1113 bp

>NTDB_id=962398 WKR14_RS15040 WP_101055493.1 3327897..3329009(-) (pilU) [Shewanella chilikensis strain ST10]
ATGGACGTCAGACCTTTTCTCAAGGCTATGGTGGAGCGCAAGGCCTCGGATCTCTTTATTACCGCCGCCTTTCCTCCCAG
TGCCAAGGTGGACGGTGAACTCAGGCCGTTGGCGGAAAACGCCTTTACCCCGGCGCAGTCGCTGGAGTTTGTCGAATCTT
TGATGACGCCGGAGCAGAAAAAGGAGTTTCACGAGACCCGTGAATGTAACTTTGCCGTGGGCGCCAAGGAGCTGGGGCGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCGGCAGGCTGCGTGATGCGGCGCATCGAAACCAAGATCCCTACAGTGGA
GCAGTTGTACCTGCCGCCCATTCTCAAAGACTTGGTGATGAGCAAGCGCGGATTGGTGATCATGGTCGGGGGAACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCACTTATCGGTTATCGCAATGCCAATGCCAGGGGACATATACTCACCATCGAAGAT
CCGGTGGAATTTGTTCACCAACATGGCAAGAGCATTATCACCCAGCGGGAAGTGGGTATAGATACCGAATCTTTTGATGC
AGCGCTCAAGAGCTCATTGCGCCAGGCGCCGGATGTTATCCTTATCGGTGAGATCCGTACCCAGGAAACCATGGAGTTTG
CTCTGTCGTTCGCTGAAACCGGCCATTTGTGTATGGCAACTCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATGCACCTGGTGCCTGAGAGTAAGCACCAACAGCTGTTGTTTGACCTGTCGCTTAACCTGCGCGGTATTGTGGCCCAGCA
GTTGGTGCCCAAGGTGGACGGCACAGGTCGGCGCGCCGCCATCGAAGTGCTTATCAACACTCCCAGAGTGGCCAGTCTTA
TTCAAAAGAACGAGCTGCACGTGCTCAAGGAGACCATGGCCAAGTCCAACGAGCAGGGGATGCAAACCTTCGATCAGGCG
CTGTTCAAGCTCTACTGTGAAGGGGAGATCAGCTACGCCGATGCACTGCATCATGCTGACTCGCCTAACGACCTGCGACT
CATGATTAAACTCCACAGCAATGACACCGGCAACTCAGGTTTCATGGAGGGAGTCACCCTGGATATGGACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.995	100	0.622
pilU	Vibrio cholerae strain A1552	58.378	100	0.584
pilU	Acinetobacter baylyi ADP1	59.943	95.135	0.57
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.58	93.243	0.378
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	42.424	89.189	0.378
pilT	Vibrio cholerae strain A1552	42.424	89.189	0.378
pilT	Legionella pneumophila strain Lp02	41.994	89.459	0.376
pilT	Legionella pneumophila strain ERS1305867	41.994	89.459	0.376
pilT	Acinetobacter baumannii D1279779	40.643	92.432	0.376
pilT	Acinetobacter baumannii strain A118	40.643	92.432	0.376
pilT	Acinetobacter nosocomialis M2	40.643	92.432	0.376
pilT	Acinetobacter baylyi ADP1	39.474	92.432	0.365
pilT	Pseudomonas stutzeri DSM 10701	38.841	93.243	0.362
pilT	Pseudomonas aeruginosa PAK	38.841	93.243	0.362