Detailed information    

experimental Experimentally validated

Overview

Name   uvrA   Type   Machinery gene
Locus tag   SPD_RS00975 Genome accession   NC_008533
Coordinates   179215..182046 (+) Length   943 a.a.
NCBI ID   WP_001152899.1    Uniprot ID   P63384
Organism   Streptococcus pneumoniae D39     
Function   homologous recombination   
Homologous recombination

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.


Genomic Context

Location: 174215..187046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPD_RS00950 (SPD_0171) - 174766..175329 (+) 564 WP_000166586.1 DNA-3-methyladenine glycosylase I -
  SPD_RS00955 (SPD_0172) - 175326..176003 (+) 678 WP_000987422.1 CPBP family intramembrane glutamic endopeptidase -
  SPD_RS00960 (SPD_0173) - 176158..177189 (-) 1032 WP_000255098.1 S66 family peptidase -
  SPD_RS00965 (SPD_0174) - 177444..178127 (-) 684 WP_000078022.1 DUF1129 domain-containing protein -
  SPD_RS00970 (SPD_0175) - 178139..179083 (-) 945 WP_000815629.1 magnesium transporter CorA family protein -
  SPD_RS00975 (SPD_0176) uvrA 179215..182046 (+) 2832 WP_001152899.1 excinuclease ABC subunit UvrA Machinery gene
  SPD_RS00980 (SPD_0177) - 182039..183100 (+) 1062 WP_001042854.1 M24 family metallopeptidase -
  SPD_RS00990 (SPD_0178) spx 183232..183630 (+) 399 WP_000591165.1 transcriptional regulator Spx -
  SPD_RS00995 (SPD_0179) - 183696..184265 (+) 570 WP_000725127.1 SP0191 family lipoprotein -
  SPD_RS01000 (SPD_0180) - 184352..184618 (+) 267 WP_000507059.1 IreB family regulatory phosphoprotein -
  SPD_RS01005 (SPD_0181) ruvX 184622..185041 (+) 420 WP_001808830.1 Holliday junction resolvase RuvX -
  SPD_RS01010 (SPD_0182) - 185057..185362 (+) 306 WP_000017620.1 DUF1292 domain-containing protein -

Sequence

Protein


Download         Length: 943 a.a.        Molecular weight: 104185.31 Da        Isoelectric Point: 6.3544

>NTDB_id=686 SPD_RS00975 WP_001152899.1 179215..182046(+) (uvrA) [Streptococcus pneumoniae D39]
MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFDTLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGL
SPAISIDQKTTSKNPRSTVGTTTEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRLFDSIEAALRIAEGYVIIDTMDD
SELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCSECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNML
EQAMKVFGVAMDKPFEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYTRTQMRLYM
NELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTLSENEAIIARPILKEIKDRLTFLNNVGLNYL
TLSRSAGTLSGGESQRIRLATQIGSNLSGVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLI
DVGPGAGVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNLQNVTARFPLGKFIAVT
GVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHVDRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNE
AKIRGYKKGRFSFNVKGGRCEACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVEFF
QHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYILDEPTTGLHTEDIARLLKVLARFVD
DGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGTIIVTGTPEEVAANEASYTGHYLKGKLHHE

Nucleotide


Download         Length: 2832 bp        

>NTDB_id=686 SPD_RS00975 WP_001152899.1 179215..182046(+) (uvrA) [Streptococcus pneumoniae D39]
ATGCAAGATAAAATTGTCATTCATGGGGCGCGTGCCCATAATTTAAAAAATATTGATGTGGAGATTCCGCGAGACAAGTT
GGTTGTCGTGACTGGCTTGTCAGGTTCAGGGAAGTCCAGTCTGGCTTTTGATACTCTCTATGCGGAGGGACAACGTCGCT
ATGTAGAGAGTTTGTCCGCCTACGCTCGTCAGTTCTTGGGAAATATGGAAAAACCAGATGTAGATGCCATTGATGGTCTC
AGCCCAGCTATTTCCATCGACCAGAAAACGACTAGTAAAAATCCTCGCTCGACGGTGGGAACAACGACTGAAATCAATGA
CTATCTGCGTCTCCTCTACGCACGTGTGGGGACGCCTTACTGTATCAACGGACATGGAGCTATCAATGCTTCTTCTGTGG
AGCAAATCGTTGATAAGGTTTTGGAGTTACCTGAACGCCAGCGCTTGCAGATCTTGGCTCCTGTCATCCGCAAGAAAAAA
GGCCAACATAAGAGTGTTATCGAGAAGGTTCAGAAAGACGGGTATGTTCGTGTCCGTGTGGATGGGGAAGTCTATGATGT
GACCGAAGTGCCAGAGTTGTCTAAGAGCAAGCAACACAATATCGATGTCGTGGTTGACCGTATTGTTATCAAGGAGGGCA
TTCGTAGTCGTCTCTTTGATTCCATTGAGGCTGCCCTTCGTATTGCAGAAGGTTATGTCATTATCGACACCATGGACGAC
TCTGAGTTGTTGTTCTCTGAGCATTATGCCTGTCCAGTTTGTGGTTTTACTGTCCCAGAGTTAGAGCCTCGTCTCTTTTC
TTTCAATGCTCCTTTTGGCTCTTGTAGTGAGTGTGACGGCTTGGGCATCAAGCTGGAGGTGGATACTGATTTGGTAGTGC
CAGATGCTAGCAAAACCTTACGTGAGGGAGCGCTAGCACCTTGGAATCCTATCTCATCCAACTACTATCCAAACATGTTA
GAGCAGGCCATGAAAGTCTTTGGAGTGGCTATGGATAAGCCTTTTGAGGACCTGTCAGAAGAAGATAAGAACTTGATTCT
CTATGGGTCAGATGGTAAGGAATTCCATTTCCACTATGAGAATGAATTTGGTGGCGTGCGCGATATCGACATTCCGTTTG
AGGGAGTTATCAATAATATCAAACGTCGTTACCATGAAACCAATAGCGATTACACTCGCACTCAGATGCGTCTCTACATG
AATGAGCTGACCTGCGGAACCTGTCAAGGCTATCGTCTCAATGACCAGGCCTTGTCTGTCCGTGTGGGCGGCCAGCAAGG
GCCACATATTGGAGAAATCTCAGACCTGTCTATCGCTGACCACTTGGACTTGGTGAGCCAGTTAACTTTGTCTGAAAATG
AAGCCATCATTGCTCGTCCCATTCTCAAGGAAATCAAGGATCGTTTGACCTTCCTTAATAATGTGGGTCTTAACTATCTA
ACCCTGTCACGTTCAGCAGGAACCCTTTCAGGTGGGGAAAGTCAGCGCATTCGTTTGGCAACCCAGATTGGTTCCAACCT
ATCAGGCGTCCTCTATATCCTAGACGAGCCGTCAATCGGTCTTCACCAGAGGGACAATGACCGCCTGATTGCCAGTCTGA
AAAAGATGCGTGACTTGGGCAATACTCTTATCGTGGTGGAACACGACGAAGATACCATGCGTGAAGCTGATTATCTGATT
GACGTTGGTCCTGGTGCCGGTGTTTTTGGTGGTGAGATTGTTGCAGCAGGTACACCTAAGCAAGTGGCTCGCAACAGCAA
GTCTATCACAGGTCAGTACTTGTCAGGCAAACGTGTCATCCCAGTACCAGAAGAGCGCCGTGTCGGAAATGGTCGTTTTA
TTGAAGTGATAGGAGCGCGTGAGAACAACTTGCAAAATGTCACGGCTCGCTTTCCACTAGGAAAATTTATCGCAGTGACA
GGTGTGTCGGGTTCAGGGAAATCGACTCTAATCAACAGCATTCTCAAAAAAGCCATTGCCCAGAAGCTCAACCGTAATTC
AGACAAACCTGGTAAGTTTAAGACTATTACAGGGATTGAGCATGTCGACCGTTTGATTGATATTGACCAGAGCCCTATCG
GACGGACGCCGAGGTCTAACCCAGCTACCTATACAGGAGTTTTTGACGATATACGTGACCTTTTTGCTCAGACAAATGAA
GCCAAAATTCGAGGCTACAAGAAGGGGCGCTTCAGTTTCAACGTCAAGGGAGGTCGCTGTGAAGCTTGCTCAGGTGACGG
GATTATCAAGATTGAGATGCACTTCTTGCCAGATGTTTATGTGGCTTGTGAAGTTTGCCACGGAACCCGCTATAACAGTG
AAACCCTAGAAGTTCACTACAAGGAAAAGAATATTTCGCAGGTCTTGGATATGACGGTCAACGATGCGGTAGAATTTTTC
CAACACATTCCGAAAATTCAACGCAAACTCCAGACCATCAAGGATGTAGGGCTAGGCTATGTGACCTTGGGACAGCCAGC
TACCACCCTTTCTGGGGGAGAAGCCCAACGTATGAAGTTAGCTAGCGAACTCCACAAACGCTCGACAGGAAAATCTTTCT
ACATTCTGGATGAGCCGACGACAGGGCTTCATACAGAGGACATTGCTCGCCTGCTCAAGGTTTTAGCTCGCTTTGTAGAC
GATGGCAATACAGTCCTCGTCATCGAGCACAATCTGGATGTTATCAAGACGGCAGACCATATCATTGACTTGGGACCTGA
GGGCGGTGTCGGTGGTGGAACCATCATCGTAACAGGAACTCCAGAAGAAGTAGCTGCCAACGAAGCCAGCTATACAGGAC
ACTATTTGAAAGGAAAGTTACATCATGAATAA

Domains

Predicted by InterProScan.

UvrA interaction domain (UvrA_inter)

(132-238)

ABC transporter (ABC_tran)

(624-860)

UvrA DNA-binding domain (UvrA_DNA-bind)

(288-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P63384

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrA Streptococcus pneumoniae R6

100

100

1
  uvrA Streptococcus pneumoniae TIGR4

100

100

1

References

[1] Joel A Lanie et al. (2007) Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6. Journal of Bacteriology 189(1):38-51. [PMID: 17041037]