Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WDB71_RS02150 Genome accession   NZ_CP149925
Coordinates   423209..424243 (+) Length   344 a.a.
NCBI ID   WP_341503007.1    Uniprot ID   -
Organism   Gallaecimonas sp. GXIMD4217     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418209..429243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDB71_RS02115 (WDB71_02115) hemW 418553..419701 (-) 1149 WP_341503000.1 radical SAM family heme chaperone HemW -
  WDB71_RS02120 (WDB71_02120) - 419701..420291 (-) 591 WP_341503001.1 XTP/dITP diphosphatase -
  WDB71_RS02125 (WDB71_02125) - 420325..420765 (-) 441 WP_341503002.1 DUF4426 domain-containing protein -
  WDB71_RS02130 (WDB71_02130) yggU 420820..421104 (-) 285 WP_341503003.1 DUF167 family protein YggU -
  WDB71_RS02135 (WDB71_02135) - 421115..421663 (-) 549 WP_341503004.1 YggT family protein -
  WDB71_RS02140 (WDB71_02140) proC 421663..422478 (-) 816 WP_341503005.1 pyrroline-5-carboxylate reductase -
  WDB71_RS02145 (WDB71_02145) - 422481..423173 (-) 693 WP_341503006.1 YggS family pyridoxal phosphate-dependent enzyme -
  WDB71_RS02150 (WDB71_02150) pilT 423209..424243 (+) 1035 WP_341503007.1 type IV pilus twitching motility protein PilT Machinery gene
  WDB71_RS02155 (WDB71_02155) pilU 424255..425364 (+) 1110 WP_341503008.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WDB71_RS02160 (WDB71_02160) ruvX 425406..425828 (-) 423 WP_341503009.1 Holliday junction resolvase RuvX -
  WDB71_RS02165 (WDB71_02165) - 425828..426397 (-) 570 WP_341503010.1 YqgE/AlgH family protein -
  WDB71_RS02170 (WDB71_02170) gshB 426471..427406 (-) 936 WP_341503011.1 glutathione synthase -
  WDB71_RS02175 (WDB71_02175) rsmE 427419..428150 (-) 732 WP_341503012.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WDB71_RS02180 (WDB71_02180) - 428150..428827 (-) 678 WP_341504170.1 endonuclease -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38065.65 Da        Isoelectric Point: 7.0503

>NTDB_id=962215 WDB71_RS02150 WP_341503007.1 423209..424243(+) (pilT) [Gallaecimonas sp. GXIMD4217]
MDITELLAFSVKHNASDLHLSAGVPPMIRVDGDVRRINLPSLEHKEVHSLIYDIMNDKQRKEYEEHLEVDFSFEVPGVAR
FRVNAYNQSRGSAAVFRTIPSKVLSLEDLGAPAIFKDIVTKPRGLVLVTGPTGSGKSTTLAGMIDHVNDNRNDHILTIED
PIEFVHENKKCLINQREVHRDTHSFAAALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKSMVRSMLSESLQAVISQTLLKKTGGGRIAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGMSHGMQTLDQCL
KELVNRGLVNRLDARHKAVDKSAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=962215 WDB71_RS02150 WP_341503007.1 423209..424243(+) (pilT) [Gallaecimonas sp. GXIMD4217]
ATGGATATTACCGAACTGTTGGCCTTTAGCGTCAAGCACAACGCGTCCGATTTACACCTTTCCGCTGGTGTTCCACCCAT
GATCCGGGTCGATGGTGATGTCCGCCGTATCAACCTGCCGTCCCTGGAACATAAGGAAGTGCACTCGCTCATTTATGACA
TCATGAATGACAAGCAGCGCAAGGAATATGAAGAGCACCTGGAAGTGGATTTCTCCTTCGAGGTGCCCGGCGTGGCCCGT
TTCAGGGTTAACGCCTACAACCAGAGCCGCGGCTCCGCGGCCGTGTTCAGGACCATCCCCTCCAAGGTGCTGAGCCTGGA
GGATCTGGGCGCCCCTGCCATCTTCAAGGATATCGTCACCAAGCCCAGGGGCCTGGTGCTGGTCACCGGGCCCACGGGTT
CCGGTAAGTCCACCACCCTGGCGGGCATGATCGACCACGTCAACGACAACAGGAACGATCACATCCTGACCATCGAAGAC
CCCATCGAATTCGTCCACGAAAACAAGAAGTGCCTGATCAACCAGCGGGAAGTGCACCGCGACACCCACAGCTTTGCCGC
CGCCCTGCGTTCGGCGCTGCGTGAAGACCCCGACGTGATCCTGGTGGGTGAGATGCGGGATCTGGAAACCATCCGCCTGG
CCCTGACCGCGGCCGAAACCGGTCACCTGGTGTTCGGTACCCTGCACACCAGCTCCGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCGGCCGGCGAGAAATCCATGGTCCGTTCCATGTTGTCGGAATCCCTGCAGGCGGTTATCTCTCAGAC
CCTGCTCAAGAAGACCGGCGGTGGCCGTATCGCCGCCCACGAGATCATGATCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCCCAGATGTATTCCGCCATCCAGACCGGTATGTCCCACGGCATGCAGACCCTGGACCAGTGCCTG
AAGGAGCTGGTCAACCGGGGCCTGGTCAATCGCCTGGATGCCCGCCACAAGGCGGTGGATAAGAGCGCTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.523

100

0.805
  pilT Acinetobacter baumannii D1279779

80.523

100

0.805
  pilT Acinetobacter baumannii strain A118

80.523

100

0.805
  pilT Pseudomonas aeruginosa PAK

81.361

98.256

0.799
  pilT Pseudomonas stutzeri DSM 10701

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

74.709

100

0.747
  pilT Legionella pneumophila strain ERS1305867

74.709

100

0.747
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.754

100

0.73
  pilT Vibrio cholerae strain A1552

72.754

100

0.73
  pilT Neisseria meningitidis 8013

69.617

98.547

0.686
  pilT Neisseria gonorrhoeae MS11

69.322

98.547

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Acinetobacter baylyi ADP1

41.964

97.674

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395