Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WDB71_RS02155 Genome accession   NZ_CP149925
Coordinates   424255..425364 (+) Length   369 a.a.
NCBI ID   WP_341503008.1    Uniprot ID   -
Organism   Gallaecimonas sp. GXIMD4217     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 419255..430364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDB71_RS02120 (WDB71_02120) - 419701..420291 (-) 591 WP_341503001.1 XTP/dITP diphosphatase -
  WDB71_RS02125 (WDB71_02125) - 420325..420765 (-) 441 WP_341503002.1 DUF4426 domain-containing protein -
  WDB71_RS02130 (WDB71_02130) yggU 420820..421104 (-) 285 WP_341503003.1 DUF167 family protein YggU -
  WDB71_RS02135 (WDB71_02135) - 421115..421663 (-) 549 WP_341503004.1 YggT family protein -
  WDB71_RS02140 (WDB71_02140) proC 421663..422478 (-) 816 WP_341503005.1 pyrroline-5-carboxylate reductase -
  WDB71_RS02145 (WDB71_02145) - 422481..423173 (-) 693 WP_341503006.1 YggS family pyridoxal phosphate-dependent enzyme -
  WDB71_RS02150 (WDB71_02150) pilT 423209..424243 (+) 1035 WP_341503007.1 type IV pilus twitching motility protein PilT Machinery gene
  WDB71_RS02155 (WDB71_02155) pilU 424255..425364 (+) 1110 WP_341503008.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WDB71_RS02160 (WDB71_02160) ruvX 425406..425828 (-) 423 WP_341503009.1 Holliday junction resolvase RuvX -
  WDB71_RS02165 (WDB71_02165) - 425828..426397 (-) 570 WP_341503010.1 YqgE/AlgH family protein -
  WDB71_RS02170 (WDB71_02170) gshB 426471..427406 (-) 936 WP_341503011.1 glutathione synthase -
  WDB71_RS02175 (WDB71_02175) rsmE 427419..428150 (-) 732 WP_341503012.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WDB71_RS02180 (WDB71_02180) - 428150..428827 (-) 678 WP_341504170.1 endonuclease -
  WDB71_RS02185 (WDB71_02185) fghA 429108..429932 (+) 825 WP_341503013.1 S-formylglutathione hydrolase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40798.04 Da        Isoelectric Point: 6.2461

>NTDB_id=962216 WDB71_RS02155 WP_341503008.1 424255..425364(+) (pilU) [Gallaecimonas sp. GXIMD4217]
MDMKSLLHRMAELEASDLFITAGLPPSIKVNGEIRPVTDQRLGLAEAKAMVESIMNDKQKAEFAQQNDCNFAINARGIGR
FRVSAFVQREAAGMVLRRIQTEIPTVEELSLPPILKDLAMTKRGLVLMVGATGTGKSTSLASMVGYRNENARGHIITIED
PIEFVHEHRKSIITQREVGIDCDSFDVALKNTLRQAPDVILLGEIRTIETMEFALAFAETGHLCMATLHANNANQALERV
LHLCPKDKREQFLFDMSLNLKGVVAQQLIPKKDGSGRAVAVEVLLNSPLVSEQIRKNELHLLKDTMERSNELGMRTFDQA
IFELYSKGVITYADALHYADSPNDLRLMIKLSKGEAGSSASLENVTLGD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=962216 WDB71_RS02155 WP_341503008.1 424255..425364(+) (pilU) [Gallaecimonas sp. GXIMD4217]
ATGGATATGAAGAGTCTGCTGCATCGCATGGCCGAGCTGGAAGCTTCGGACCTCTTTATCACGGCCGGCCTGCCGCCTTC
CATCAAGGTCAACGGCGAGATCCGGCCGGTCACCGACCAGCGGCTGGGCCTGGCCGAGGCCAAGGCCATGGTCGAAAGCA
TCATGAACGACAAACAGAAGGCGGAGTTTGCCCAGCAAAACGACTGCAACTTTGCCATCAACGCCCGCGGCATAGGGCGC
TTCCGGGTGTCTGCCTTTGTACAGCGGGAAGCGGCCGGCATGGTGCTGCGCCGCATCCAGACCGAGATCCCCACGGTCGA
GGAGCTCTCTCTGCCGCCGATCCTCAAGGATCTGGCCATGACCAAGCGTGGCCTGGTGCTGATGGTGGGCGCCACCGGTA
CGGGTAAGTCCACCTCGCTGGCATCCATGGTGGGCTATCGCAACGAAAATGCCCGTGGCCACATCATCACCATCGAGGAC
CCCATCGAATTCGTCCACGAACACCGCAAGTCCATCATCACCCAGCGGGAGGTGGGCATCGATTGCGACTCCTTCGACGT
GGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGCTTGGTGAGATCCGCACCATAGAAACCATGGAGTTCG
CCCTGGCCTTCGCCGAGACGGGCCACCTCTGCATGGCGACCCTGCACGCCAACAACGCCAACCAGGCCCTGGAGCGGGTG
CTGCATCTGTGTCCCAAGGACAAGCGGGAGCAATTCCTGTTCGACATGTCCCTGAACCTCAAGGGCGTGGTGGCCCAGCA
GCTCATACCCAAGAAGGACGGCAGCGGCCGGGCCGTGGCGGTGGAGGTGCTGCTCAACTCGCCCCTGGTCAGCGAGCAGA
TCCGCAAGAACGAACTGCACCTGCTCAAGGACACCATGGAGCGCTCCAACGAGCTGGGCATGAGGACCTTTGACCAGGCC
ATCTTCGAGCTTTACAGCAAGGGTGTCATCACCTATGCCGATGCCCTGCACTATGCCGATTCGCCCAATGATCTGCGCCT
GATGATCAAGCTCAGCAAGGGTGAGGCCGGCTCCTCGGCCTCCCTTGAAAACGTCACCCTGGGTGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.736

98.645

0.629
  pilU Vibrio cholerae strain A1552

59.673

99.458

0.593
  pilU Acinetobacter baylyi ADP1

61.864

95.935

0.593
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.389

97.561

0.404
  pilT Pseudomonas aeruginosa PAK

40.29

93.496

0.377
  pilT Acinetobacter nosocomialis M2

40.643

92.683

0.377
  pilT Acinetobacter baumannii D1279779

40.351

92.683

0.374
  pilT Acinetobacter baumannii strain A118

40.351

92.683

0.374
  pilT Acinetobacter baylyi ADP1

39.474

92.683

0.366
  pilT Pseudomonas stutzeri DSM 10701

38.841

93.496

0.363