Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WE862_RS07035 Genome accession   NZ_CP149571
Coordinates   1450821..1451855 (+) Length   344 a.a.
NCBI ID   WP_033114043.1    Uniprot ID   A0AAQ0EDN6
Organism   Aeromonas jandaei strain DSM 7311     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1445821..1456855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WE862_RS07005 (WE862_07005) - 1446577..1447773 (+) 1197 WP_033114037.1 NupC/NupG family nucleoside CNT transporter -
  WE862_RS07010 (WE862_07010) - 1447875..1448294 (-) 420 WP_042031657.1 DUF4426 domain-containing protein -
  WE862_RS07015 (WE862_07015) yggU 1448324..1448623 (-) 300 WP_033114039.1 DUF167 family protein YggU -
  WE862_RS07020 (WE862_07020) - 1448623..1449174 (-) 552 WP_042031658.1 YggT family protein -
  WE862_RS07025 (WE862_07025) proC 1449186..1450013 (-) 828 WP_198493542.1 pyrroline-5-carboxylate reductase -
  WE862_RS07030 (WE862_07030) - 1450080..1450781 (-) 702 WP_042031661.1 YggS family pyridoxal phosphate-dependent enzyme -
  WE862_RS07035 (WE862_07035) pilT 1450821..1451855 (+) 1035 WP_033114043.1 type IVa pilus ATPase TapT Machinery gene
  WE862_RS07040 (WE862_07040) pilU 1451884..1452993 (+) 1110 WP_198493558.1 type IVa pilus ATPase TapU Machinery gene
  WE862_RS07045 (WE862_07045) yaaA 1453041..1453814 (+) 774 WP_198493541.1 peroxide stress protein YaaA -
  WE862_RS07050 (WE862_07050) - 1454009..1454437 (+) 429 WP_033114045.1 hypothetical protein -
  WE862_RS07055 (WE862_07055) - 1454753..1455244 (-) 492 WP_042031988.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  WE862_RS07060 (WE862_07060) arfB 1455266..1455682 (-) 417 WP_198493540.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  WE862_RS07065 (WE862_07065) glnK 1455884..1456222 (+) 339 WP_033114048.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38305.09 Da        Isoelectric Point: 6.7440

>NTDB_id=959893 WE862_RS07035 WP_033114043.1 1450821..1451855(+) (pilT) [Aeromonas jandaei strain DSM 7311]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGMVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=959893 WE862_RS07035 WP_033114043.1 1450821..1451855(+) (pilT) [Aeromonas jandaei strain DSM 7311]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCGTCGGATCTACACCTGTCCGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACATCGGGAAGTGCACGCCCTCATTTACGACA
TCATGAACGATCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTTGCCCGC
TTCCGGGTCAACGCCTTCCAGCAAGCGCGCGGTTCCGGCGCGGTATTTCGTACCATCCCCAGTACTGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGTGGTCTGGTGCTGGTGACCGGGCCGACCGGTT
CCGGTAAGTCGACCACGCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAAGAC
CCCATCGAATTTGTGCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCACCGCGATACCAAGAGTTTCAGCAA
TGCCCTGCGCTCGGCACTGCGGGAAGACCCCGACATCATTCTGGTGGGCGAGATGCGCGATCTTGAAACCATTCGCCTCG
CCATGACCGCAGCCGAAACCGGCCACCTGGTATTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCGCAGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGTATTGGCGGCGGTCGGGTGGCAGCTCACGAGATCATGATGGGGATCCCGGCGGTGCGTAACCTGATCC
GGGAGGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTCAGCCGTGGCATGGTGGCGTCTCTCGACGCCAAGGCCAAGGCGGTTGACCCCAACAGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.668

97.965

0.741
  pilT Vibrio cholerae strain A1552

75.668

97.965

0.741
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407