Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WE862_RS07040 Genome accession   NZ_CP149571
Coordinates   1451884..1452993 (+) Length   369 a.a.
NCBI ID   WP_198493558.1    Uniprot ID   -
Organism   Aeromonas jandaei strain DSM 7311     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1446884..1457993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WE862_RS07010 (WE862_07010) - 1447875..1448294 (-) 420 WP_042031657.1 DUF4426 domain-containing protein -
  WE862_RS07015 (WE862_07015) yggU 1448324..1448623 (-) 300 WP_033114039.1 DUF167 family protein YggU -
  WE862_RS07020 (WE862_07020) - 1448623..1449174 (-) 552 WP_042031658.1 YggT family protein -
  WE862_RS07025 (WE862_07025) proC 1449186..1450013 (-) 828 WP_198493542.1 pyrroline-5-carboxylate reductase -
  WE862_RS07030 (WE862_07030) - 1450080..1450781 (-) 702 WP_042031661.1 YggS family pyridoxal phosphate-dependent enzyme -
  WE862_RS07035 (WE862_07035) pilT 1450821..1451855 (+) 1035 WP_033114043.1 type IVa pilus ATPase TapT Machinery gene
  WE862_RS07040 (WE862_07040) pilU 1451884..1452993 (+) 1110 WP_198493558.1 type IVa pilus ATPase TapU Machinery gene
  WE862_RS07045 (WE862_07045) yaaA 1453041..1453814 (+) 774 WP_198493541.1 peroxide stress protein YaaA -
  WE862_RS07050 (WE862_07050) - 1454009..1454437 (+) 429 WP_033114045.1 hypothetical protein -
  WE862_RS07055 (WE862_07055) - 1454753..1455244 (-) 492 WP_042031988.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  WE862_RS07060 (WE862_07060) arfB 1455266..1455682 (-) 417 WP_198493540.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  WE862_RS07065 (WE862_07065) glnK 1455884..1456222 (+) 339 WP_033114048.1 P-II family nitrogen regulator -
  WE862_RS07070 (WE862_07070) - 1456416..1457423 (+) 1008 WP_143241803.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41074.06 Da        Isoelectric Point: 6.4422

>NTDB_id=959894 WE862_RS07040 WP_198493558.1 1451884..1452993(+) (pilU) [Aeromonas jandaei strain DSM 7311]
MNLDELLGQLVEKKGSDLFVTVGTSPTLKVNGHLLSLGGEALDKKAALTLVRETLSPEHFERYIRTREANYAIHRETLGR
FRVSAFWQQELPGMVVRRIETRIPTFDDLQLPVILQEVALAKRGLVLFVGATGAGKSTTQATMIGYRNQHADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSSETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLKAIVAQQLVPSVDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQS
LFNLFCSGQIGYSEALAHADSANDLRLLIKLSGRDQLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=959894 WE862_RS07040 WP_198493558.1 1451884..1452993(+) (pilU) [Aeromonas jandaei strain DSM 7311]
ATGAATCTGGATGAACTGCTGGGCCAGCTGGTAGAGAAAAAAGGGTCGGATCTCTTTGTGACGGTGGGGACGTCCCCCAC
CCTCAAGGTGAACGGCCATCTGCTCTCTTTGGGAGGCGAAGCGCTCGATAAAAAAGCTGCTCTTACGCTGGTGAGGGAGA
CCCTGAGCCCCGAGCATTTCGAGCGCTATATCCGCACCAGGGAGGCCAACTACGCCATTCACCGCGAAACCTTGGGCCGG
TTTCGGGTCAGCGCTTTCTGGCAGCAGGAGCTGCCGGGCATGGTGGTTAGGCGTATCGAGACACGTATTCCTACCTTCGA
TGATCTGCAACTGCCCGTGATCCTGCAGGAGGTCGCATTGGCCAAGCGTGGACTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCGACCATGATCGGTTATCGCAACCAGCATGCCGACGGCCATATTCTGACGGTGGAGGAC
CCGGTAGAGTTTGTCCATCAGCATGGCCGCAGCCTGATCACCCAGCGGGAGGTGGGGATCGATACCGAGTCGTTCGATGT
GGCGTTGAAAAGCTCGCTGCGTCAGGCGCCTGACGTCATTCTGATCGGCGAGATCAGAAGCTCTGAGACCATGGAGTTTG
CCCTGCAATTCGCCGAGACTGGTCACCTCTGCCTCGCGACCCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTACCGCAGGAGAAGCATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTCAAGGCCATTGTCGCCCAGCA
ACTGGTACCGAGTGTGGATGGCAAGCGGCGCTGTGCGGCGTTCGAGATCCTGCTGAACACCCCGCTTATCACCGACATTA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAATCGACCGAGCTTGGCATGCAAACCTTCGATCAATCG
CTCTTCAACCTGTTCTGCTCCGGCCAGATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGGCT
GCTGATCAAGCTTTCCGGCCGTGATCAGCTTGGAACTGGTACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.783

99.729

0.596
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Legionella pneumophila strain Lp02

39.942

92.954

0.371
  pilT Legionella pneumophila strain ERS1305867

39.942

92.954

0.371
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.413

91.87

0.371
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36