Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WIR04_RS17865 Genome accession   NZ_CP149130
Coordinates   3928846..3929880 (+) Length   344 a.a.
NCBI ID   WP_025325664.1    Uniprot ID   -
Organism   Aeromonas rivipollensis strain BE1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3923846..3934880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIR04_RS17825 - 3924023..3924397 (+) 375 WP_025325672.1 YacL family protein -
  WIR04_RS17830 - 3924448..3924744 (+) 297 WP_025325671.1 hypothetical protein -
  WIR04_RS17835 - 3924939..3925769 (+) 831 WP_338888705.1 CPBP family intramembrane glutamic endopeptidase -
  WIR04_RS17840 - 3925847..3926266 (-) 420 WP_338888707.1 DUF4426 domain-containing protein -
  WIR04_RS17845 yggU 3926271..3926579 (-) 309 WP_025325668.1 DUF167 family protein YggU -
  WIR04_RS17850 - 3926579..3927130 (-) 552 WP_025325667.1 YggT family protein -
  WIR04_RS17855 proC 3927154..3927978 (-) 825 WP_338888711.1 pyrroline-5-carboxylate reductase -
  WIR04_RS17860 - 3928105..3928806 (-) 702 WP_338888713.1 YggS family pyridoxal phosphate-dependent enzyme -
  WIR04_RS17865 pilT 3928846..3929880 (+) 1035 WP_025325664.1 type IVa pilus ATPase TapT Machinery gene
  WIR04_RS17870 pilU 3929907..3931016 (+) 1110 WP_338888716.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WIR04_RS17875 yaaA 3931049..3931822 (+) 774 WP_338888718.1 peroxide stress protein YaaA -
  WIR04_RS17880 srmB 3932005..3933228 (-) 1224 WP_025325661.1 ATP-dependent RNA helicase SrmB -
  WIR04_RS17885 - 3933357..3934067 (+) 711 WP_338888720.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38159.89 Da        Isoelectric Point: 6.7438

>NTDB_id=956775 WIR04_RS17865 WP_025325664.1 3928846..3929880(+) (pilT) [Aeromonas rivipollensis strain BE1]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLEDLGAPEMFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFANALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVAALDARAKAADPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=956775 WIR04_RS17865 WP_025325664.1 3928846..3929880(+) (pilT) [Aeromonas rivipollensis strain BE1]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGTGAAGTACACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAGCTGGAGGAGAATTTCGAGGTGGACTTCTCCTTCGAGGTACCCGGCATGGCGCGC
TTTCGGGTCAACGCCTTCCAGCAGGCGCGAGGCTCTGGCGCCGTGTTTCGTACCATACCGAGCACAGTCCTGACCCTGGA
AGATCTTGGGGCGCCGGAGATGTTTCGCAAGATAGCGGAGTTTCCCCGCGGCCTGGTGCTGGTCACGGGCCCGACCGGTT
CGGGCAAGTCCACCACGCTCGCGGCCATGGTCGATTACATCAACGACAACTTCCACCACCACATCCTCACCATCGAGGAT
CCCATCGAGTTCGTGCACGAGAACAAGCGTTGCCTGGTCAACCAGCGCGAGGTACATCGCGACACCAAGAGTTTCGCCAA
CGCCCTGCGCTCGGCCCTGCGTGAAGATCCGGACATCATACTGGTGGGGGAAATGCGCGACCTGGAGACCATACGCCTCG
CCATGACGGCGGCGGAGACGGGCCATCTGGTGTTCGGCACCCTGCACACCTCCTCGGCGGCCAAGACCATAGACAGGATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGAGATCCATGCTCTCCGAGTCCCTGCGGGCTGTCATCTCCCAGAC
CCTGCTCAAGCGGATCGGCGGTGGCCGGGTGGCGGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTGATCC
GGGAAGACAAGGTCGCCCAGCTCTACTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACAATGGATCAGAGCCTC
AAGCAGCTCGTCAACCGGGGCGTGGTTGCCGCCCTCGATGCCAGGGCCAAGGCGGCCGACCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

78.426

99.709

0.782
  pilT Acinetobacter baumannii D1279779

77.326

100

0.773
  pilT Acinetobacter baumannii strain A118

77.326

100

0.773
  pilT Pseudomonas stutzeri DSM 10701

77.326

100

0.773
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Pseudomonas aeruginosa PAK

76.453

100

0.765
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

73.333

95.93

0.703
  pilT Legionella pneumophila strain ERS1305867

73.333

95.93

0.703
  pilT Neisseria meningitidis 8013

68.696

100

0.689
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.686
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.77

99.709

0.526
  pilU Vibrio cholerae strain A1552

43.478

100

0.436
  pilU Pseudomonas stutzeri DSM 10701

41.449

100

0.416
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401