Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WIR04_RS17870 Genome accession   NZ_CP149130
Coordinates   3929907..3931016 (+) Length   369 a.a.
NCBI ID   WP_338888716.1    Uniprot ID   -
Organism   Aeromonas rivipollensis strain BE1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3924907..3936016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIR04_RS17835 - 3924939..3925769 (+) 831 WP_338888705.1 CPBP family intramembrane glutamic endopeptidase -
  WIR04_RS17840 - 3925847..3926266 (-) 420 WP_338888707.1 DUF4426 domain-containing protein -
  WIR04_RS17845 yggU 3926271..3926579 (-) 309 WP_025325668.1 DUF167 family protein YggU -
  WIR04_RS17850 - 3926579..3927130 (-) 552 WP_025325667.1 YggT family protein -
  WIR04_RS17855 proC 3927154..3927978 (-) 825 WP_338888711.1 pyrroline-5-carboxylate reductase -
  WIR04_RS17860 - 3928105..3928806 (-) 702 WP_338888713.1 YggS family pyridoxal phosphate-dependent enzyme -
  WIR04_RS17865 pilT 3928846..3929880 (+) 1035 WP_025325664.1 type IVa pilus ATPase TapT Machinery gene
  WIR04_RS17870 pilU 3929907..3931016 (+) 1110 WP_338888716.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WIR04_RS17875 yaaA 3931049..3931822 (+) 774 WP_338888718.1 peroxide stress protein YaaA -
  WIR04_RS17880 srmB 3932005..3933228 (-) 1224 WP_025325661.1 ATP-dependent RNA helicase SrmB -
  WIR04_RS17885 - 3933357..3934067 (+) 711 WP_338888720.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  WIR04_RS17890 brnQ 3934304..3935566 (+) 1263 WP_338892628.1 branched-chain amino acid transport system II carrier protein -
  WIR04_RS17895 - 3935654..3935881 (-) 228 WP_163135628.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41016.14 Da        Isoelectric Point: 6.2698

>NTDB_id=956776 WIR04_RS17870 WP_338888716.1 3929907..3931016(+) (pilU) [Aeromonas rivipollensis strain BE1]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVPLGDKPLDRQAALALVRESLDEAHFERYLHSREANYAIHREGLGR
FRVSAFWQQDMPGMVLRRIETRIPTFEELTLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHGDGHILTVED
PVEFVHQHGRCLVTQREVGIDTESFDVALKNSMRQAPDVILIGEIRSQETMELAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLHGQRRVAAFEILLNTPLITDIVRKGEVHRLKEVMTKSGELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=956776 WIR04_RS17870 WP_338888716.1 3929907..3931016(+) (pilU) [Aeromonas rivipollensis strain BE1]
ATGAACATGGATGTTCTGCTCGCTGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACTGTCGATGCCCCTCCCAC
CCTCAAGGTCAACGGGCGGCTGGTACCGCTCGGCGATAAACCCCTGGACAGGCAGGCGGCGCTGGCCCTGGTCAGGGAGA
GCCTGGACGAGGCGCACTTCGAGCGCTACCTGCATAGCCGGGAGGCAAACTACGCCATCCACCGTGAAGGGCTGGGGCGA
TTTCGGGTCAGCGCCTTCTGGCAGCAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATTCCCACCTTCGA
AGAACTGACGCTGCCCCCCATACTGCAGGAGGTCGCCATGGCCAAGCGCGGCCTGGTGCTGTTCGTCGGGGCAACGGGCG
CCGGCAAGTCCACTACCCAGGCGGCAATGATCGGCTACCGCAACCAGCACGGCGACGGCCACATACTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCACCAGCATGGGCGCTGCCTGGTGACCCAGCGGGAAGTGGGGATCGACACCGAGTCCTTCGACGT
GGCGCTCAAGAACTCCATGCGCCAGGCGCCGGACGTCATCCTCATCGGCGAGATCCGCAGCCAGGAGACCATGGAACTGG
CCATCCAGTTTGCCGAGACGGGCCACCTCTGTCTCGCCACCCTCCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGAGAAGCATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATAGTGGCACAGCA
ACTGCTGCCCTCCCTTCACGGGCAGCGCCGTGTCGCCGCCTTCGAGATACTGCTCAACACCCCGCTCATTACCGACATAG
TCCGCAAGGGAGAGGTACACAGGCTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGTCGCGAGCAGCTGGGTGCAGGCACACTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Legionella pneumophila strain Lp02

41.399

92.954

0.385
  pilT Legionella pneumophila strain ERS1305867

41.399

92.954

0.385
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.071

91.057

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Pseudomonas stutzeri DSM 10701

38.889

92.683

0.36
  pilT Pseudomonas aeruginosa PAK

39.349

91.599

0.36