Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WJM78_RS17570 Genome accession   NZ_CP149129
Coordinates   3822305..3823339 (+) Length   344 a.a.
NCBI ID   WP_005320466.1    Uniprot ID   A0AAP4JDF8
Organism   Aeromonas popoffii strain BE5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3817305..3828339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM78_RS17540 - 3818962..3819261 (+) 300 WP_042036155.1 hypothetical protein -
  WJM78_RS17545 - 3819332..3819751 (-) 420 WP_212512767.1 DUF4426 domain-containing protein -
  WJM78_RS17550 yggU 3819770..3820069 (-) 300 WP_042036158.1 DUF167 family protein YggU -
  WJM78_RS17555 - 3820069..3820620 (-) 552 WP_108536333.1 YggT family protein -
  WJM78_RS17560 proC 3820649..3821473 (-) 825 WP_275930702.1 pyrroline-5-carboxylate reductase -
  WJM78_RS17565 - 3821564..3822265 (-) 702 WP_275930701.1 YggS family pyridoxal phosphate-dependent enzyme -
  WJM78_RS17570 pilT 3822305..3823339 (+) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  WJM78_RS17575 pilU 3823368..3824477 (+) 1110 WP_339015908.1 type IVa pilus ATPase TapU Machinery gene
  WJM78_RS17580 yaaA 3824509..3825282 (+) 774 WP_042036165.1 peroxide stress protein YaaA -
  WJM78_RS17585 srmB 3825472..3826695 (-) 1224 WP_339010830.1 ATP-dependent RNA helicase SrmB -
  WJM78_RS17590 - 3826824..3827534 (+) 711 WP_275930699.1 methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38307.00 Da        Isoelectric Point: 6.7439

>NTDB_id=956742 WJM78_RS17570 WP_005320466.1 3822305..3823339(+) (pilT) [Aeromonas popoffii strain BE5]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQSRGSGAVFRTIPSTVLSLEDLDAPEIFRKISEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSV

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=956742 WJM78_RS17570 WP_005320466.1 3822305..3823339(+) (pilT) [Aeromonas popoffii strain BE5]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCTGGGGTTCCCCCGAT
GATCCGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACAGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGC
TTCCGGGTCAACGCCTTCCAGCAATCGCGCGGTTCGGGGGCCGTGTTTCGTACCATCCCCAGCACGGTACTGAGTCTGGA
GGATCTCGATGCCCCCGAGATCTTTCGCAAGATCTCCGAATACCCCCGCGGCCTGGTGCTGGTGACAGGCCCGACCGGCT
CGGGCAAGTCCACCACACTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCACCACCACATACTCACTATCGAAGAC
CCCATCGAATTCGTACACGAGAACAAACGCTGTCTGGTGAACCAGCGGGAAGTACACCGCGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATACTGGTGGGCGAGATGCGCGATCTCGAGACAATACGTCTGG
CCATGACGGCGGCCGAGACGGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCAGCGGCCAAGACCATAGACCGTATC
ATCGACGTCTTCCCCGGCGCGGAAAAAGACATGGTGCGATCCATGCTCTCTGAATCCCTGCGCGCCGTCATCTCCCAGAC
CCTGCTCAAGCGGATCGGCGGTGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATACCCGCGGTGCGCAACCTCATCC
GGGAAGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAACTGGTCAGTCGTGGCGTGGTGGCCTCCCTGGATGCCAAGGCCAAGGCGGTCGACCCCAACAGCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.843

99.709

0.776
  pilT Acinetobacter baylyi ADP1

77.843

99.709

0.776
  pilT Acinetobacter baumannii D1279779

77.551

99.709

0.773
  pilT Acinetobacter baumannii strain A118

77.551

99.709

0.773
  pilT Pseudomonas stutzeri DSM 10701

76.385

99.709

0.762
  pilT Pseudomonas aeruginosa PAK

75.802

99.709

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.668

97.965

0.741
  pilT Vibrio cholerae strain A1552

75.668

97.965

0.741
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

42.609

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404