Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WJM78_RS17575 Genome accession   NZ_CP149129
Coordinates   3823368..3824477 (+) Length   369 a.a.
NCBI ID   WP_339015908.1    Uniprot ID   -
Organism   Aeromonas popoffii strain BE5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3818368..3829477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM78_RS17540 - 3818962..3819261 (+) 300 WP_042036155.1 hypothetical protein -
  WJM78_RS17545 - 3819332..3819751 (-) 420 WP_212512767.1 DUF4426 domain-containing protein -
  WJM78_RS17550 yggU 3819770..3820069 (-) 300 WP_042036158.1 DUF167 family protein YggU -
  WJM78_RS17555 - 3820069..3820620 (-) 552 WP_108536333.1 YggT family protein -
  WJM78_RS17560 proC 3820649..3821473 (-) 825 WP_275930702.1 pyrroline-5-carboxylate reductase -
  WJM78_RS17565 - 3821564..3822265 (-) 702 WP_275930701.1 YggS family pyridoxal phosphate-dependent enzyme -
  WJM78_RS17570 pilT 3822305..3823339 (+) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  WJM78_RS17575 pilU 3823368..3824477 (+) 1110 WP_339015908.1 type IVa pilus ATPase TapU Machinery gene
  WJM78_RS17580 yaaA 3824509..3825282 (+) 774 WP_042036165.1 peroxide stress protein YaaA -
  WJM78_RS17585 srmB 3825472..3826695 (-) 1224 WP_339010830.1 ATP-dependent RNA helicase SrmB -
  WJM78_RS17590 - 3826824..3827534 (+) 711 WP_275930699.1 methyltransferase -
  WJM78_RS17595 brnQ 3827770..3829032 (+) 1263 WP_275930698.1 branched-chain amino acid transport system II carrier protein -
  WJM78_RS17600 - 3829104..3829334 (-) 231 WP_042036171.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41086.08 Da        Isoelectric Point: 6.3187

>NTDB_id=956743 WJM78_RS17575 WP_339015908.1 3823368..3824477(+) (pilU) [Aeromonas popoffii strain BE5]
MNLDDLLTELVKRKGSDLFVTVGSPPTLKVNGHLVSLGDEALDKKGALALVRDTLSSDHFERYIRTKEANYAIHREDLGR
FRVSAFWQQELPGMVLRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSSETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSVDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=956743 WJM78_RS17575 WP_339015908.1 3823368..3824477(+) (pilU) [Aeromonas popoffii strain BE5]
ATGAATCTGGATGATCTACTGACCGAGCTGGTCAAGCGAAAGGGGTCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGACGAGGCGCTCGACAAGAAGGGGGCGCTGGCGCTGGTCAGAGATA
CCCTCAGTAGCGATCACTTCGAGCGCTACATCCGCACCAAGGAAGCTAACTACGCCATCCACCGCGAGGATCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGTTGCCTGGCATGGTACTGCGCCGCATCGAGACCCGGATCCCGACCTTCGA
CGAGCTGACCCTGCCCCCCATCTTGCAGGAGGTGGCCATGGCCAAGCGCGGGCTGGTGCTGTTTGTCGGCGCCACCGGTG
CGGGCAAGTCCACCACCCAGGCCGCCATGATAGGGTTTCGCAACCAGCATGCCGATGGCCATATACTGACGGTGGAAGAC
CCGGTGGAGTTCGTGCACCAGCACGGTCGCAGCCTGATCACCCAGCGGGAGGTGGGGATAGACACAGAGTCGTTCGACGT
GGCACTGAAGAGTTCGCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGAAGCTCTGAGACCATGGAGTTTG
CGCTGCAATTTGCCGAGACGGGTCACCTCTGTCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCACTGGATCGCATC
CTCCACCTAGTGCCACAGGAGAAACACCGACAGTTTCTGTTTGATCTCTCCTTCAACCTGCGCGCCATCGTGGCCCAGCA
ACTGCTTCCGAGCGTGGACGGTCGCCGCCGTTTTGCGGCCTTCGAAGTACTGCTCAACACCCCGCTCATTACCGACATCA
TCCGCAAGGGGGAGATGCATCGCCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACTCTGTTCTGTGCTGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGCTGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.405

95.122

0.556
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.003

91.87

0.377
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Neisseria gonorrhoeae MS11

38.218

94.309

0.36