Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WJM28_RS18170 Genome accession   NZ_CP149125
Coordinates   3941160..3942194 (+) Length   344 a.a.
NCBI ID   WP_010673050.1    Uniprot ID   A0A081LS93
Organism   Aeromonas caviae strain BE3     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3936160..3947194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM28_RS18130 - 3936377..3936751 (+) 375 WP_134997683.1 YacL family protein -
  WJM28_RS18135 - 3936801..3937091 (+) 291 WP_039039963.1 hypothetical protein -
  WJM28_RS18140 - 3937253..3938083 (+) 831 WP_134997682.1 CPBP family intramembrane glutamic endopeptidase -
  WJM28_RS18145 - 3938159..3938578 (-) 420 WP_134997681.1 DUF4426 domain-containing protein -
  WJM28_RS18150 yggU 3938597..3938896 (-) 300 WP_134997680.1 DUF167 family protein YggU -
  WJM28_RS18155 - 3938896..3939447 (-) 552 WP_042014922.1 YggT family protein -
  WJM28_RS18160 proC 3939471..3940295 (-) 825 WP_134997679.1 pyrroline-5-carboxylate reductase -
  WJM28_RS18165 - 3940419..3941120 (-) 702 WP_049636471.1 YggS family pyridoxal phosphate-dependent enzyme -
  WJM28_RS18170 pilT 3941160..3942194 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  WJM28_RS18175 pilU 3942218..3943327 (+) 1110 WP_042882153.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WJM28_RS18180 yaaA 3943360..3944133 (+) 774 WP_042882155.1 peroxide stress protein YaaA -
  WJM28_RS18185 srmB 3944321..3945541 (-) 1221 WP_339331822.1 ATP-dependent RNA helicase SrmB -
  WJM28_RS18190 - 3945669..3946379 (+) 711 WP_039039957.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38189.90 Da        Isoelectric Point: 6.7436

>NTDB_id=956688 WJM28_RS18170 WP_010673050.1 3941160..3942194(+) (pilT) [Aeromonas caviae strain BE3]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLDDLGAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVASLDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=956688 WJM28_RS18170 WP_010673050.1 3941160..3942194(+) (pilT) [Aeromonas caviae strain BE3]
ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTCCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGGAAGTCCATGCTCTCATTTACGACA
TCATGAATGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAAGTACCCGGCATGGCACGC
TTTCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCCGTGTTTCGTACCATCCCGAGTACCGTTCTGACCCTGGA
CGATCTGGGGGCGCCGGAGATATTTCGCAAGATAGCGGAGTACCCTCGCGGCCTGGTACTGGTGACTGGGCCCACCGGCT
CGGGCAAATCCACCACCCTGGCCGCCATGGTCGATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAC
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGATATCATCCTGGTAGGTGAAATGCGGGACCTCGAAACCATCCGCCTCG
CCATGACCGCCGCCGAAACCGGTCACCTGGTGTTTGGCACCCTGCACACCTCCTCGGCTGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGATCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTGGCCCAGCTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAGCAGCTGGTCAACCGGGGGGTCGTCGCCTCCCTTGATGCCAAGGCCAAGGCTGTCGATCCCAATACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081LS93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Pseudomonas stutzeri DSM 10701

78.299

99.128

0.776
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Pseudomonas aeruginosa PAK

76.833

99.128

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

71.471

98.837

0.706
  pilT Legionella pneumophila strain ERS1305867

71.471

98.837

0.706
  pilT Neisseria meningitidis 8013

69.027

98.547

0.68
  pilT Neisseria gonorrhoeae MS11

68.732

98.547

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.924

99.419

0.526
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407