Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WJM28_RS18175 Genome accession   NZ_CP149125
Coordinates   3942218..3943327 (+) Length   369 a.a.
NCBI ID   WP_042882153.1    Uniprot ID   A0A2L0TPJ0
Organism   Aeromonas caviae strain BE3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3937218..3948327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM28_RS18140 - 3937253..3938083 (+) 831 WP_134997682.1 CPBP family intramembrane glutamic endopeptidase -
  WJM28_RS18145 - 3938159..3938578 (-) 420 WP_134997681.1 DUF4426 domain-containing protein -
  WJM28_RS18150 yggU 3938597..3938896 (-) 300 WP_134997680.1 DUF167 family protein YggU -
  WJM28_RS18155 - 3938896..3939447 (-) 552 WP_042014922.1 YggT family protein -
  WJM28_RS18160 proC 3939471..3940295 (-) 825 WP_134997679.1 pyrroline-5-carboxylate reductase -
  WJM28_RS18165 - 3940419..3941120 (-) 702 WP_049636471.1 YggS family pyridoxal phosphate-dependent enzyme -
  WJM28_RS18170 pilT 3941160..3942194 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  WJM28_RS18175 pilU 3942218..3943327 (+) 1110 WP_042882153.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WJM28_RS18180 yaaA 3943360..3944133 (+) 774 WP_042882155.1 peroxide stress protein YaaA -
  WJM28_RS18185 srmB 3944321..3945541 (-) 1221 WP_339331822.1 ATP-dependent RNA helicase SrmB -
  WJM28_RS18190 - 3945669..3946379 (+) 711 WP_039039957.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  WJM28_RS18195 brnQ 3946569..3947876 (+) 1308 WP_339331823.1 branched-chain amino acid transport system II carrier protein -
  WJM28_RS18200 - 3947966..3948193 (-) 228 WP_128344179.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40901.04 Da        Isoelectric Point: 6.3155

>NTDB_id=956689 WJM28_RS18175 WP_042882153.1 3942218..3943327(+) (pilU) [Aeromonas caviae strain BE3]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=956689 WJM28_RS18175 WP_042882153.1 3942218..3943327(+) (pilU) [Aeromonas caviae strain BE3]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGAGAAA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGACGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCTCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACAGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTACGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TPJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379