Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFHSH190041_RS14240 Genome accession   NZ_AP026070
Coordinates   3208414..3209451 (-) Length   345 a.a.
NCBI ID   WP_261922430.1    Uniprot ID   -
Organism   Shewanella sp. NFH-SH190041     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3203414..3214451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFHSH190041_RS14210 (NFHSH190041_27220) yciH 3203819..3204148 (+) 330 WP_261925136.1 stress response translation initiation inhibitor YciH -
  NFHSH190041_RS14215 (NFHSH190041_27230) - 3204172..3204732 (+) 561 WP_261922425.1 YqgE/AlgH family protein -
  NFHSH190041_RS14220 (NFHSH190041_27240) ruvX 3204747..3205169 (+) 423 WP_261922426.1 Holliday junction resolvase RuvX -
  NFHSH190041_RS14225 (NFHSH190041_27250) hemH 3205286..3206302 (-) 1017 WP_261922427.1 ferrochelatase -
  NFHSH190041_RS14230 (NFHSH190041_27260) - 3206345..3206974 (-) 630 WP_261922428.1 glutathione peroxidase -
  NFHSH190041_RS14235 (NFHSH190041_27270) pilU 3207292..3208404 (-) 1113 WP_261922429.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NFHSH190041_RS14240 (NFHSH190041_27280) pilT 3208414..3209451 (-) 1038 WP_261922430.1 type IV pilus twitching motility protein PilT Machinery gene
  NFHSH190041_RS14245 (NFHSH190041_27290) - 3209487..3210179 (+) 693 WP_261922431.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFHSH190041_RS14250 (NFHSH190041_27300) proC 3210531..3211349 (+) 819 WP_261922432.1 pyrroline-5-carboxylate reductase -
  NFHSH190041_RS14255 (NFHSH190041_27310) - 3211395..3211943 (+) 549 WP_261922433.1 YggT family protein -
  NFHSH190041_RS14260 (NFHSH190041_27320) yggU 3211954..3212241 (+) 288 WP_261922434.1 DUF167 family protein YggU -
  NFHSH190041_RS14265 (NFHSH190041_27330) - 3212340..3212774 (+) 435 WP_261922435.1 DUF4426 domain-containing protein -
  NFHSH190041_RS14270 (NFHSH190041_27340) rdgB 3213088..3213687 (+) 600 WP_261922436.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38228.72 Da        Isoelectric Point: 6.7118

>NTDB_id=95661 NFHSH190041_RS14240 WP_261922430.1 3208414..3209451(-) (pilT) [Shewanella sp. NFH-SH190041]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGDVRKINVPALDHEGVHSLVYDIMNDRQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQARGAAAVFRTIPSEVLSLEALAAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMIDYINENRHDHILTIED
PIEFVHSNKHCLINQREVHRHTHSFNAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRIAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGQITREDALAKSSNRQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=95661 NFHSH190041_RS14240 WP_261922430.1 3208414..3209451(-) (pilT) [Shewanella sp. NFH-SH190041]
ATGGAAATCACTGAGTTATTAGCTTTTAGTGTAAAGCACAACGCATCTGATCTGCACCTTTCTGCCGGGGTATCTCCTAT
GATCCGGGTCGACGGTGATGTGAGAAAAATCAATGTTCCCGCGCTGGATCATGAAGGCGTCCACAGTCTGGTGTACGACA
TTATGAATGACCGGCAGCGCAAAGACTACGAAGAGCATTTGGAAATTGATTTTTCATTCGAAGTGCCGAATCTGGCTCGT
TTCCGGGTTAATGCTTTTAATCAGGCTCGTGGTGCGGCCGCTGTATTTCGGACGATTCCCAGTGAAGTGTTATCTCTGGA
AGCATTAGCCGCGCCGGAAATCTTCAAAAAAATTGCCAGTTTTCCTCGGGGGTTAGTATTGGTCACCGGCCCGACCGGTT
CGGGTAAAAGTACCACGTTGGCTGCCATGATTGATTACATCAATGAAAATCGCCATGACCATATTTTGACTATTGAAGAC
CCGATTGAATTTGTGCACAGCAATAAGCATTGTCTGATCAATCAACGGGAAGTGCACCGCCATACCCATAGCTTCAATGC
TGCGCTGCGCAGCGCGTTGCGTGAAGATCCGGATGTGATTTTAGTGGGAGAATTGCGGGATTTGGAAACCATACGCTTGG
CGATGACAGCTGCCGAAACCGGGCATCTGGTATTTGGTACCTTGCATACCACCTCTGCCGCTAAAACCATTGACCGGGTA
GTGGATGTGTTTCCCGCCGGAGAAAAAGATATGGTGCGTACCATGCTGTCAGAATCGCTGCAGGCTGTGATTTCTCAAAC
CTTGATTAAAAAGGTTGGCGGGGGCCGAATTGCCGCCCATGAAATTATGATGGGCACGCCAGCCATTCGTAACCTGATTC
GCGAAGACAAGGTAGCACAGATGTATTCTGCGATTCAGACCGGCATGGCCCATGGAATGCAGACCTTGGAGCAGTGCCTG
CAGAATCTGGTTAACCGTGGCCAAATTACCCGAGAGGATGCTCTGGCTAAAAGCAGTAACCGTCAGGCCACTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.24

96.812

0.777
  pilT Acinetobacter baumannii strain A118

80.24

96.812

0.777
  pilT Acinetobacter nosocomialis M2

80.24

96.812

0.777
  pilT Pseudomonas aeruginosa PAK

77.515

97.971

0.759
  pilT Acinetobacter baylyi ADP1

77.976

97.391

0.759
  pilT Pseudomonas stutzeri DSM 10701

77.219

97.971

0.757
  pilT Legionella pneumophila strain Lp02

75.146

99.13

0.745
  pilT Legionella pneumophila strain ERS1305867

75.146

99.13

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.681

0.716
  pilT Vibrio cholerae strain A1552

73.294

97.681

0.716
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.566

99.42

0.423
  pilU Vibrio cholerae strain A1552

40.816

99.42

0.406
  pilU Acinetobacter baylyi ADP1

39.466

97.681

0.386

Multiple sequence alignment