Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NFHSH190041_RS14235 Genome accession   NZ_AP026070
Coordinates   3207292..3208404 (-) Length   370 a.a.
NCBI ID   WP_261922429.1    Uniprot ID   -
Organism   Shewanella sp. NFH-SH190041     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3202292..3213404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFHSH190041_RS14205 (NFHSH190041_27210) - 3202994..3203569 (-) 576 WP_261925135.1 DUF4136 domain-containing protein -
  NFHSH190041_RS14210 (NFHSH190041_27220) yciH 3203819..3204148 (+) 330 WP_261925136.1 stress response translation initiation inhibitor YciH -
  NFHSH190041_RS14215 (NFHSH190041_27230) - 3204172..3204732 (+) 561 WP_261922425.1 YqgE/AlgH family protein -
  NFHSH190041_RS14220 (NFHSH190041_27240) ruvX 3204747..3205169 (+) 423 WP_261922426.1 Holliday junction resolvase RuvX -
  NFHSH190041_RS14225 (NFHSH190041_27250) hemH 3205286..3206302 (-) 1017 WP_261922427.1 ferrochelatase -
  NFHSH190041_RS14230 (NFHSH190041_27260) - 3206345..3206974 (-) 630 WP_261922428.1 glutathione peroxidase -
  NFHSH190041_RS14235 (NFHSH190041_27270) pilU 3207292..3208404 (-) 1113 WP_261922429.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NFHSH190041_RS14240 (NFHSH190041_27280) pilT 3208414..3209451 (-) 1038 WP_261922430.1 type IV pilus twitching motility protein PilT Machinery gene
  NFHSH190041_RS14245 (NFHSH190041_27290) - 3209487..3210179 (+) 693 WP_261922431.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFHSH190041_RS14250 (NFHSH190041_27300) proC 3210531..3211349 (+) 819 WP_261922432.1 pyrroline-5-carboxylate reductase -
  NFHSH190041_RS14255 (NFHSH190041_27310) - 3211395..3211943 (+) 549 WP_261922433.1 YggT family protein -
  NFHSH190041_RS14260 (NFHSH190041_27320) yggU 3211954..3212241 (+) 288 WP_261922434.1 DUF167 family protein YggU -
  NFHSH190041_RS14265 (NFHSH190041_27330) - 3212340..3212774 (+) 435 WP_261922435.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40689.57 Da        Isoelectric Point: 5.8887

>NTDB_id=95660 NFHSH190041_RS14235 WP_261922429.1 3207292..3208404(-) (pilU) [Shewanella sp. NFH-SH190041]
MDVRPLLSTMVERKASDLFITAGFPPSAKIDGELTPLSDAALTPEQSLALVHSVMTDAQQQEFSQSKECNFAFAVKGLGR
FRVSAFWQRESCGCVMRRIETRIPTVEELHLPQILKDLVMSKRGLVIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHNHNQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPENKHHQLLFDLSLNLKAIVAQQLIPRIDGNGRRAAIEVLINTPRVASLIQNNELHLIKETMAKSTEQGMQTFDQA
LFSLYQEGEISYADALHCADSPNDLRLMIKLASSGQGSSGMMDGVTIDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=95660 NFHSH190041_RS14235 WP_261922429.1 3207292..3208404(-) (pilU) [Shewanella sp. NFH-SH190041]
ATGGATGTTCGTCCCCTATTAAGTACCATGGTGGAGCGTAAAGCCTCGGATCTGTTTATTACCGCAGGCTTTCCCCCCAG
TGCCAAAATCGATGGTGAGCTAACACCATTATCCGATGCTGCCCTCACCCCAGAGCAATCGCTAGCCTTGGTGCACTCGG
TGATGACTGACGCCCAGCAACAGGAGTTCAGTCAGAGTAAAGAATGTAATTTCGCTTTTGCAGTCAAAGGATTGGGACGA
TTTCGTGTCAGTGCTTTTTGGCAGCGTGAGTCTTGTGGCTGTGTGATGCGCCGGATTGAAACCCGTATTCCTACTGTTGA
GGAGCTGCATTTACCACAAATCCTGAAAGATTTGGTGATGAGCAAACGAGGGCTGGTGATTATGGTGGGTGGCACCGGCA
CGGGTAAGTCGACCTCACTGGCTGCCTTAGTCGGGTATCGTAATGCCCACGCCCGCGGCCATATTCTTACCATTGAAGAT
CCGGTGGAATTTGTTCATAACCACAATCAAAGCATTATCACCCAACGAGAAGTTGGCATAGATACCGAAAGCTTCGATGC
CGCGCTGAAAAGCTCTTTGCGACAGGCGCCGGATGTGATTTTGATTGGTGAGATCCGCAGCCAAGAAACCATGGAATTTG
CCCTGTCCTTTGCTGAGACAGGTCATTTATGTATGGCAACCCTGCACGCCAATAATGCCAACCAGGCATTAGATCGAATT
ATGCATTTGGTACCGGAGAATAAACATCACCAGTTGCTGTTTGATCTGTCATTAAATCTTAAAGCCATTGTGGCGCAGCA
GCTGATCCCCCGCATCGACGGTAACGGTCGCCGGGCTGCGATTGAAGTACTGATTAATACCCCAAGGGTGGCAAGTCTTA
TCCAGAATAATGAGTTGCATCTGATCAAAGAGACCATGGCTAAATCCACAGAGCAGGGGATGCAGACCTTTGATCAGGCG
CTATTCAGTCTGTATCAGGAAGGGGAGATCAGCTATGCCGATGCCCTGCACTGTGCCGACTCACCAAATGATTTGCGCTT
GATGATCAAATTGGCCAGCAGTGGCCAGGGCAGCTCTGGCATGATGGATGGTGTCACTATCGACATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.647

100

0.608
  pilU Vibrio cholerae strain A1552

60

100

0.6
  pilU Acinetobacter baylyi ADP1

61.236

96.216

0.589
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.382

97.568

0.414
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.207

88.649

0.392
  pilT Vibrio cholerae strain A1552

44.207

88.649

0.392
  pilT Acinetobacter baumannii D1279779

40.643

92.432

0.376
  pilT Acinetobacter nosocomialis M2

40.643

92.432

0.376
  pilT Acinetobacter baumannii strain A118

40.643

92.432

0.376
  pilT Acinetobacter baylyi ADP1

40.351

92.432

0.373
  pilT Pseudomonas aeruginosa PAK

40

93.243

0.373
  pilT Legionella pneumophila strain ERS1305867

40.413

91.622

0.37
  pilT Legionella pneumophila strain Lp02

40.413

91.622

0.37
  pilT Neisseria meningitidis 8013

40.785

89.459

0.365
  pilT Neisseria gonorrhoeae MS11

40.785

89.459

0.365
  pilT Pseudomonas stutzeri DSM 10701

38.841

93.243

0.362

Multiple sequence alignment