Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V8J88_RS02180 Genome accession   NZ_CP147776
Coordinates   559102..560148 (+) Length   348 a.a.
NCBI ID   WP_338847502.1    Uniprot ID   -
Organism   Massilia sp. W12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 554102..565148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8J88_RS02160 (V8J88_02160) - 554912..555982 (+) 1071 WP_338847499.1 hypothetical protein -
  V8J88_RS02165 (V8J88_02165) - 556143..557258 (-) 1116 WP_338847500.1 succinylglutamate desuccinylase/aspartoacylase family protein -
  V8J88_RS02170 (V8J88_02170) proC 557332..558150 (-) 819 WP_338849984.1 pyrroline-5-carboxylate reductase -
  V8J88_RS02175 (V8J88_02175) - 558191..558907 (-) 717 WP_338847501.1 YggS family pyridoxal phosphate-dependent enzyme -
  V8J88_RS02180 (V8J88_02180) pilT 559102..560148 (+) 1047 WP_338847502.1 type IV pilus twitching motility protein PilT Machinery gene
  V8J88_RS02185 (V8J88_02185) pilU 560166..561302 (+) 1137 WP_338847503.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V8J88_RS02190 (V8J88_02190) - 561302..561787 (+) 486 WP_338847504.1 glutathione peroxidase -
  V8J88_RS02195 (V8J88_02195) ybaL 562205..563896 (+) 1692 WP_338847505.1 YbaL family putative K(+) efflux transporter -
  V8J88_RS02200 (V8J88_02200) - 563977..564246 (-) 270 WP_338847506.1 hypothetical protein -
  V8J88_RS02205 (V8J88_02205) - 564315..564557 (-) 243 WP_338847507.1 hypothetical protein -
  V8J88_RS02210 (V8J88_02210) - 564561..565052 (-) 492 WP_338847508.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38049.91 Da        Isoelectric Point: 6.8884

>NTDB_id=948529 V8J88_RS02180 WP_338847502.1 559102..560148(+) (pilT) [Massilia sp. W12]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMVYDIMNDAQRKTYEEMLEIDFSFAIPGLAR
FRVNAFNQDRGAAAVLRTIPSKILSLEDLKCPKIFAELALKPRGLVLVTGPTGSGKSTTLAAMINHLNENEYAHILTIED
PIEFVHDSKKCLINQREVGPHTLSFAAALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMLGTPAIRNLIREAKVAQMYSAIQTGSNVGMQTLDAN
LADLVKRNIISNSTARFAAKSPENFPGA

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=948529 V8J88_RS02180 WP_338847502.1 559102..560148(+) (pilT) [Massilia sp. W12]
ATGGACATTTCCGAACTGTTAGCTTTCTCTGTCAAGAACAAAGCTTCCGACTTACACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAACCTGCCCCCGCTTGAGCACAAAGACGTGCATGGCATGGTGTATGACA
TCATGAACGACGCCCAGCGCAAAACCTATGAGGAAATGCTGGAAATCGACTTTTCTTTTGCGATTCCCGGCCTGGCGCGT
TTCCGTGTCAATGCTTTTAACCAGGATCGCGGCGCGGCCGCCGTGCTGCGTACCATTCCTTCCAAGATCCTGTCCCTGGA
AGATTTGAAATGCCCGAAAATTTTCGCCGAGCTGGCTTTGAAGCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAATCGACCACGCTGGCGGCCATGATCAACCATTTGAATGAAAATGAATACGCGCACATTCTGACCATCGAAGAC
CCGATTGAATTTGTGCACGACTCGAAAAAATGTCTGATCAACCAGCGCGAAGTCGGCCCGCACACTCTGTCGTTCGCCGC
CGCCCTGCGCTCCGCGCTGCGTGAAGATCCGGATGCGATTCTGGTGGGCGAGTTGCGTGATCTGGAAACCATCCGCCTGG
CCATGACCGCAGCTGAAACCGGTCACTTGGTGTTCGGCACCCTGCACACCTCATCCGCTGCGAAAACGATTGACCGTATC
ATCGACGTCTTCCCCGGCGAAGAAAAAGAAATGATTCGCGCCATGTTGTCGGAATCGCTGCAGGCGGTGATTTCGCAAAC
CCTGCTCAAAACCAAGGACGGTTCGGGCCGTGTGGCGGCGCATGAAATCATGTTGGGCACCCCGGCAATCCGCAATCTGA
TCCGCGAAGCCAAAGTGGCGCAGATGTATTCCGCGATTCAAACCGGCTCGAATGTCGGCATGCAAACCCTGGATGCCAAT
CTGGCCGATCTGGTCAAGCGCAATATCATTTCCAATTCGACAGCGCGCTTTGCCGCCAAATCGCCGGAAAACTTCCCCGG
CGCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.391

99.138

0.767
  pilT Acinetobacter baumannii D1279779

77.391

99.138

0.767
  pilT Acinetobacter baumannii strain A118

77.391

99.138

0.767
  pilT Pseudomonas aeruginosa PAK

75.652

99.138

0.75
  pilT Legionella pneumophila strain ERS1305867

76.106

97.414

0.741
  pilT Legionella pneumophila strain Lp02

76.106

97.414

0.741
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.138

0.739
  pilT Acinetobacter baylyi ADP1

74.203

99.138

0.736
  pilT Neisseria meningitidis 8013

68.406

99.138

0.678
  pilT Neisseria gonorrhoeae MS11

68.116

99.138

0.675
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.091

94.828

0.655
  pilT Vibrio cholerae strain A1552

69.091

94.828

0.655
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.138

0.52
  pilU Pseudomonas stutzeri DSM 10701

41.449

99.138

0.411
  pilU Vibrio cholerae strain A1552

41.449

99.138

0.411
  pilU Acinetobacter baylyi ADP1

41.329

99.425

0.411