Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V8J88_RS02185 Genome accession   NZ_CP147776
Coordinates   560166..561302 (+) Length   378 a.a.
NCBI ID   WP_338847503.1    Uniprot ID   -
Organism   Massilia sp. W12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 555166..566302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8J88_RS02165 (V8J88_02165) - 556143..557258 (-) 1116 WP_338847500.1 succinylglutamate desuccinylase/aspartoacylase family protein -
  V8J88_RS02170 (V8J88_02170) proC 557332..558150 (-) 819 WP_338849984.1 pyrroline-5-carboxylate reductase -
  V8J88_RS02175 (V8J88_02175) - 558191..558907 (-) 717 WP_338847501.1 YggS family pyridoxal phosphate-dependent enzyme -
  V8J88_RS02180 (V8J88_02180) pilT 559102..560148 (+) 1047 WP_338847502.1 type IV pilus twitching motility protein PilT Machinery gene
  V8J88_RS02185 (V8J88_02185) pilU 560166..561302 (+) 1137 WP_338847503.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V8J88_RS02190 (V8J88_02190) - 561302..561787 (+) 486 WP_338847504.1 glutathione peroxidase -
  V8J88_RS02195 (V8J88_02195) ybaL 562205..563896 (+) 1692 WP_338847505.1 YbaL family putative K(+) efflux transporter -
  V8J88_RS02200 (V8J88_02200) - 563977..564246 (-) 270 WP_338847506.1 hypothetical protein -
  V8J88_RS02205 (V8J88_02205) - 564315..564557 (-) 243 WP_338847507.1 hypothetical protein -
  V8J88_RS02210 (V8J88_02210) - 564561..565052 (-) 492 WP_338847508.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42007.49 Da        Isoelectric Point: 6.9600

>NTDB_id=948530 V8J88_RS02185 WP_338847503.1 560166..561302(+) (pilU) [Massilia sp. W12]
MERDQATRFMFDLLRLMVSKNGSDLFLTSGFPPAIKIDGKMTPVSNQALTATHTTELARAIMNDKQAATFELSKECNFAI
SPGDLGRFRVSAFVQLGCVGMVVRTITTTIPKIEDLGLPPVLRDVVMSKRGLVIMVGATGSGKSTTLAAMLGYRNENSYG
HIITIEDPVEYVHPHKNCIVTQREVGVDTDSWEAALKNSLRQAPDVIQIGEIRDRETMDHAIAFAETGHLCLATLHANSS
NQALDRIINFFPEERRQQLLMDLSLNLRAMISQRLIPRRDTKGRCAAIEIMLNSPFISDLIFKGAVHEIKEVMKKSRELG
MQTFDQSLFDLFEADMITYEDALRNADSLNDLRLAIKLKGKAAKQRDLSAGTEHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=948530 V8J88_RS02185 WP_338847503.1 560166..561302(+) (pilU) [Massilia sp. W12]
ATGGAACGCGATCAAGCCACCCGCTTTATGTTCGACCTGCTGCGCCTGATGGTCAGCAAGAATGGTTCGGACTTATTTTT
AACCTCCGGCTTCCCGCCGGCCATCAAGATCGACGGCAAAATGACGCCGGTCTCCAATCAGGCCCTGACGGCGACCCACA
CCACCGAGTTGGCGCGCGCGATCATGAACGACAAACAGGCCGCCACCTTCGAGCTGAGCAAGGAATGCAACTTCGCCATC
AGCCCGGGCGATCTGGGGCGCTTCCGCGTTTCGGCCTTTGTGCAGCTGGGTTGCGTTGGCATGGTGGTGCGCACCATCAC
CACCACCATCCCGAAAATCGAAGACCTCGGTTTGCCGCCGGTATTGCGCGATGTGGTGATGAGCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACCGGCTCCGGTAAATCCACCACGCTGGCGGCGATGCTGGGCTATCGCAATGAAAACAGCTATGGC
CACATCATCACGATTGAAGATCCGGTGGAATATGTGCACCCGCATAAAAACTGCATCGTCACGCAGCGCGAAGTCGGGGT
CGATACCGATTCCTGGGAAGCAGCGCTGAAAAACTCGCTGCGGCAAGCGCCGGACGTGATCCAGATCGGCGAAATCCGCG
ACCGCGAGACCATGGACCATGCGATCGCCTTCGCCGAAACCGGCCACCTGTGTTTGGCCACCCTGCACGCGAACAGCTCA
AACCAGGCGCTTGACCGCATCATCAACTTCTTCCCGGAAGAGCGGCGCCAGCAATTATTAATGGACTTGTCGCTCAATCT
GCGCGCCATGATTTCGCAGCGCCTGATTCCGCGCCGCGACACCAAGGGACGTTGCGCGGCGATTGAAATCATGCTGAATT
CACCTTTCATTTCCGATTTGATTTTCAAGGGTGCGGTGCATGAAATCAAAGAAGTCATGAAAAAATCACGCGAACTGGGC
ATGCAAACCTTTGACCAGTCGCTGTTTGATCTGTTTGAAGCTGACATGATCACTTACGAAGACGCCCTGCGCAACGCCGA
TTCTCTGAACGACTTGCGTCTGGCGATCAAACTCAAGGGCAAGGCCGCCAAACAGCGCGACTTGTCCGCCGGCACTGAAC
ATTTGGGGATTGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.162

91.534

0.587
  pilU Acinetobacter baylyi ADP1

57.703

94.444

0.545
  pilU Vibrio cholerae strain A1552

55.977

90.741

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.672

88.624

0.405
  pilT Pseudomonas stutzeri DSM 10701

42.687

88.624

0.378
  pilT Pseudomonas aeruginosa PAK

42.388

88.624

0.376
  pilT Acinetobacter baumannii D1279779

42.9

87.566

0.376
  pilT Acinetobacter baumannii strain A118

42.9

87.566

0.376
  pilT Acinetobacter nosocomialis M2

42.9

87.566

0.376
  pilT Acinetobacter baylyi ADP1

42.9

87.566

0.376
  pilT Legionella pneumophila strain ERS1305867

41.39

87.566

0.362
  pilT Legionella pneumophila strain Lp02

41.39

87.566

0.362